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Biomedical subjects

A H Vermeulen

Publications and source records attributed to A H Vermeulen.

9 recordsLinked to original sources
PubMed1989

Histochemical detection of osteocalcin in normal and pathological human bone.

We investigated the immunohistochemical localization of osteocalcin in demineralized, paraffin-embedded normal and pathological human bone. Acid decalcification protocols appeared to be more suitable for osteocalcin detection than mild chelating agents. In normal lamellar bone, osteocalcin was detected in osteocytes and along the lamellar bone matrix in fine granular deposits. Under pathological conditions (osteomyelitis, neoplasia), appositional bone showed immunoreactivity in osteoblasts and osteocytes but not in the provisory woven bone matrix. Intense immunoreactivity could be seen at the cell borders of osteoclasts and the bone margins of Howship lacunae. In primary bone-forming tumors, osteocalcin immunoreactivity was detected in osteoblasts and their malignant counterparts. On the basis of these results, we conclude that optimal preservation of osteocalcin is obtained through mild acid decalcifiers. Osteocalcin is deposited in bone matrix, especially that of metabolically inactive bone. In neoplasms, osteocalcin could be a marker of osteoblastic differentiation.

Bone Neoplasms↗
PubMed1982

IUD and salpingitis: a prospective study of pathomorphological changes in the oviducts in IUD-users.

A prospective study on pathomorphological changes in the oviducts in 253 women who underwent voluntary sterilization via posterior colpotomy performed at the Catharina Ziekenhuis, Eindhoven, was performed in order to investigate the effect of the intrauterine device (IUD). Some form of salpingitis was found in 54% of the IUD-group and in only 6% of the control group (P less than 10(-6), four-fold table, chi-squared test.) Microbiological study in 51 patients did not explain the pathoanatomical changes. As the IUD-and control-groups were comparable in all investigated parameters, the inflammatory reaction must be caused by the method of contraception.

Adult↗