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Biomedical subjects

A Hönig

Publications and source records attributed to A Hönig.

7 recordsLinked to original sources
PubMed1998

[Surgical treatment of Crohn disease based on the inflammatory pattern].

286 patients with Crohn's disease were classified on the basis of the inflammatory pattern at their first operation as type 1 (Ileitis: n = 116), type 2a (segmental colitis: n = 60), and type 2b (total colitis: n = 108); 2 patients remained unclassified. At the same age at operation of 31.9 +/- 10.7 yrs symptoms were known in type 1 for 3.4 +/- 3.9 yrs, but for 7.5 +/- 5.7 yrs in type 2b. Main indication in type 1 was stenosis (56.9%), whereas in type 2b intractabilitiy (68.5%) predominated. Type 2a was intermediate concerning duration of symptoms and relationship of indications including fistulas. Standard-procedures were ileocecal resection (92.2%) in type 1, and colectomy (90.7%) in type 2b. In type 2a ileocolic resections and partial colectomies were mostly done. During the following 3.9 +/- 3.8 yrs reoperation rate due to disease progression was 13.6% in type 1, 25.5% in type 2a and 18.5% in type 2b. The cumulative risk of ileal resection at ten years due to new inflammation was significantly (p < 0.01) higher in the case of ileocolic/ileorectal anastomosis than of ileostomy (38% vs. 11%). In contrast, cumulative probability of a colorectal resection was significantly (p < 0.05) higher in type 2 (16%) when compared to type 1 (1.5%). Primary ileal loss was significantly (p < 0.01) higher in type 1 (37 +/- 23 cm) compared with type 2a (25 +/- 28 cm) and type 2b (17 +/- 21 cm). Loss of continence occurred in 0%, 3.3% and 53.7% respectively. With reoperations additional loss of ileum decreased in all types, whereas in type 2 loss of anorectal function increased. Including reoperations the rate of major complications was 9.8% and lethality was 0.8% (3/386). Resections in Crohn's disease are unavoidable due to shrinking therapeutical alternatives in the course of the disease. Owing to limited resections, loss of bowel may not exceed ileum in type 1, whereas the same resectional policy cannot avoid the total loss of the colorectum eventually in type 2. Both limited surgery and repeated resections help to maintain function as long as possible. Due to the high safety-standard the number of operations does not impair the success of the surgical concept.

Adolescent↗
PubMed1990

[The effectiveness and safety of an immunization against parvovirus and rabies in anesthetized puppies].

The efficacy and safety of a vaccination during anaesthesia was examined in 20 puppies from 8 litters of one kennel. The animals were vaccinated in a state of reflex-free anaesthesia (neuroleptanalgesia in combination with halothane, nitrous oxide and oxygen). 20 puppies from the same litters were used as controls. A parvovirus living vaccine (Canimed) and a rabies vaccine from inactivated viruses (Rabisin) were used for the vaccinations. The titers of neutralizing antibodies against rabies virus were significantly lower in the group of anaesthetized animals compared to the control group on the 10th and 20th day p.vacc. The average titers of antibodies against parvovirus of both groups, however, do not allow any statistically significant statements. It is nevertheless remarkable that 6 of the anaesthetized puppies (three different litters) showed no increase in antibodies. The results of the investigations lead to the recommendation not to vaccinate anaesthetized puppies. The risk of reducing the efficacy of vaccinations with vaccines from inactivated agents is greater than with living vaccines.

Anesthesia↗
PubMedDate not supplied

Cystosarcoma phyllodes of the breast: prognostic significance of proliferation and apoptosis associated genes.

BACKGROUND: Cystosarcoma phyllodes (CP) is a rare fibroepithelial tumor of the breast. So far no valuable prognostic factors are available for predicting the biological behaviour and often unexpected clinical outcome of this tumor. PATIENTS AND METHODS: Using immuno-histochemistry, Mib-1 evaluated proliferative activity and expression of p53, Bcl-2, c-erbB1, c-erbB2, cytokeratin and vimentin were analysed in 17 benign and 12 malignant CP. RESULTS: Cell proliferation was significantly higher in malignant tumors compared to benign ones both in the epithelial and mesenchymal parts. In the epithelial part Bcl-2 positive cells were found more frequently in benign than in malignant lesions. Positive immunoreactivity for p53 was seen in only one malignant case. The reaction was positive in the both epithelial and mesenchymal compartments. Expression of c-erbB-proteins was not found in any case. The intermediate filament proteins indicate clearly the two components of the tumor. CONCLUSIONS: We concluded that increased proliferate activity and downregulation of Bcl-2 may be associated with dedifferentiation of cells and malignant progression. C-erbB-proteins are obviously not involved in carcinogenesis of CP.

Apoptosis↗
PubMedDate not supplied

Helicobacter pylori-induced hyperproliferation: relevance for gastric cancer development in connection with mutagenic factors.

AIMS: Current data on Helicobacter pylori induced regenerative hyperproliferation of the antral gastric mucosa and significance in cancer development are still under discussion and investigation. An improved method for evaluation of the regeneratory process in antral mucosa is introduced and compared with the conventional method used for determination of proliferating cells in perpendicular sections of the gastric mucosa. METHODS: Using a combination of immunohistochemistry and PAS-staining the expression of Ki-67 (MiB1)-proliferation associated antigen was analyzed in 50 Helicobacter pylori (Hp) positive and 35 negative biopsies of the gastric antrum. PAS-staining was performed to identify the proliferative zone of the antral gastric glands. The degree of inflammation was evaluated by grades on routinely H & E-stained slides. RESULTS: Proliferative activity is significantly increased in Hp-positive cases (p = 0.00095) compared to negative ones. By using the conventionally applied proliferation index, every nucleus has to be counted and the proliferation zone is identified by at least one positive stained nucleus. The method presented here seems to be easier because the proliferative zone is clearly identified by PAS staining of neutral glycoproteins characteristic for the proliferation zone of antral glands. The density of labeled nuclei is determined and is expressed as a proliferation factor. This factor gives more distinct values, is easier to evaluate and shows a better correlation with the helicobacter status and the degree of inflammation. These results are discussed in relation to the data from the literature and with a view to other relevant factors in the course of carcinogenesis, such as growth factors, mainly EGF, p53 mutation and role of apoptosis, genetic instability and local production of oxidants. CONCLUSION: Helicobacter pylori induces an increase of regenerative proliferation activity. Under these conditions the chance of mutation is increased and time for DNA repair reduced. This could be at least a part of multiple step carcinogenesis. The newly introduced combination of staining procedures (PAS/MiBI) allows a more differentiated evaluation of the proliferation zone and its widening. This method can be more easily handled in follow-up studies than the method using perpendicular sections because in this method heavy irregularities of gland pattern induced by accompanying inflammatory processes considerably hinder evaluation.

Cell Division↗