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Biomedical subjects

A Hadidi

Publications and source records attributed to A Hadidi.

41 records · Page 3Linked to original sources
PubMedDate not supplied

Scar skin and dapple apple viroids are seed-borne and persistent in infected apple trees.

The closely related apple scar skin viroid (ASSV) and dapple apple viroid (DAV) were identified in whole seeds from infected pome fruits by hybridization of extracted nucleic acids with a 32P-labelled ASSV cRNA probe. Viroid amounts were greater in seed coats and subcoats than in seed cotyledons and embryos. ASSV or DAV was also detected in nucleic acid extracts from infected seeds, cotyledons and embryos by reverse transcription/polymerase chain reaction with viroid-cDNA-specific primers followed by Southern blot hybridization analysis of the amplified products with an ASSV cRNA probe. These results indicate that ASSV and DAV are seed-borne. ASSV and DAV were also found in the anthers, petals, receptacles, leaves, bark and roots of infected trees. The results suggest that viroid-infected trees constitute potential sources of the viroid in field spread. ASSV and DAV infections have been observed sporadically in commercial orchards in the United States and Canada and the infected trees have been eliminated. The use of viroid-free sources of seeds, seedlings, rootstocks and budwood should greatly reduce the risk of the future spread of the viroid.

Animals↗
PubMedDate not supplied

Magnetic resonance cholangiopancreatography in patients with upper abdominal pain: a prospective study.

BACKGROUND/AIMS: To determine the role of magnetic resonance cholangiopancreatogram with conventional abdominal magnetic resonance examination in patients presenting clinically with upper abdominal pain and abnormal liver function tests and to compare the findings with endoscopic retrograde cholangiopancreatogram results. METHODOLOGY: Magnetic resonance cholangiopancreatogram and endoscopic retrograde cholangiopancreatogram were done in 77 patients. Conventional magnetic resonance examination of the liver and upper abdomen was done first followed by magnetic resonance cholangiopancreatogram using a half fourrier single shot turbo spin echo sequence. Conventional endoscopic retrograde cholangiopancreatogram was done by direct intraductal injection of radiographic contrast material through a duodenoscope under fluoroscopy control. RESULTS: Endoscopic retrograde cholangiopancreatogram failed in 7 patients (9%) and Magnetic resonance cholangiopancreatogram images were inadequate in 3 patients (4%). The findings of adequate magnetic resonance exams in 74 patients were: choledocholithiasis in 24 patients (32%), bile duct stricture in 19 patients (26%), normal biliary ducts in 29 patients (39%) and dilated biliary ducts with no definite cause in 2 patients (3%). The findings of successful endoscopic retrograde cholangiopancreatograms in 67 patients after exclusion of inadequate magnetic resonance cholangiopancreatograms were: choledocholithiasis in 25 patients (37%), bile duct stricture in 18 patients (27%), normal biliary ducts in 21 patients (31%) and dilated biliary ducts with no evident cause in 2 patients (3%) and hemobilia in 1 patient (2%). CONCLUSIONS: Magnetic resonance cholangiopancreatogram is a non-invasive technique, its accuracy is increased if it is combined with conventional abdominal magnetic resonance exam and it can replace the endoscopic retrograde cholangiopancreatogram.

Abdominal Pain↗
PubMedDate not supplied

Ischaemic stroke in Jordan: a 2-year hospital-based study of subtypes and risk factors.

A retrospective study was made of 200 consecutive patients with first-ever ischaemic stroke, admitted to Jordan University Hospital over a 2-year period. The mean age was 61.2 years (range 29-95). The most common stroke subtype was lacunar infarct (51.5%), but frequency of cardioembolic stroke was low (8.0%). Hypertension, diabetes mellitus and smoking were the most common risk factors for atherosclerotic non-cardioembolic stroke. Chronic atrial fibrillation was the most common risk factor for cardioembolic stroke. No patient had severe extracranial carotid or vertebral artery stenosis (> 50% narrowing). Lacunar strokes presented predominantly as pure motor stroke (67/103) and were mainly in the internal capsule (34/ 103). The favourable outcome (85% discharged home) may be due to the relatively young age and the predominance of lacunar infarcts.

Adult↗