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A Hapel

Publications and source records attributed to A Hapel.

5 recordsLinked to original sources
PubMed1994

Effects of stem cell factor (kit-ligand) and interleukin-3 on the growth and serine proteinase expression of rat bone-marrow-derived or serosal mast cells.

The effects of rat stem-cell factor (SCF) and interleukin-3 (IL-3), alone or in combination, on the in vitro growth and serine proteinase expression of rat serosal/connective-tissue mast cells (CTMC) or bone marrow-derived mast cells (BMMC) were examined. Rat SCF stimulated the growth of both CTMC and BMMC. IL-3 stimulated BMMC growth to a lesser extent than did SCF, whereas CTMC numbers did not increase in IL-3. However, SCF and IL-3 had synergistic effects on the growth of both BMMC and CTMC. SCF favoured the maintenance of rat mast cell proteinase-I (RMCP-I) in CTMC, but did not induce detectable production of RMCP-I in BMMC. In contrast, when IL-3 or lymph node-conditioned medium (LNCM) was added to SCF, a subpopulation of CTMC expressed and stored the soluble proteinase RMCP-II. In BMMC, the RMCP-II content of cells maintained in SCF was significantly less than that of cells maintained in IL-3 or LNCM. RMCP-II also appeared in the supernatants of BMMC, especially when BMMC numbers were increasing rapidly in SCF with or without IL-3 or LNCM. Thus, SCF and IL-3 can regulate the growth of rat BMMC and CTMC, as well as influence their production and release of proteinases.

Animals↗
PubMed1983

Interleukin 3-dependent hematopoietic progenitor cell lines.

Several biological phenotypes of growth factor-dependent cell lines have been described in recent years, including those with T lymphocyte, neutrophil granulocyte, basophil/mast cell, B lymphocyte, and multipotential stem cell properties. The growth factors for each cell lineage are a subject of intense study. Continuous mouse bone marrow cultures infected with RNA type C viruses (retroviruses) produce nonadherent hematopoietic cells over a longer duration than control cultures. Marrow cultures derived from strains with spontaneously induced ecotropic endogenous retrovirus demonstrate a greater longevity than those from strains with no replicating virus. Cultures infected with murine leukemia virus also generate a greater number, compared with controls, of cloned permanent suspension cell lines dependent for growth on a 41,000-dalton glycoprotein (interleukin 3 [IL 3]). Some are multipotential with capacity for differentiation to erythroid, neutrophil, eosinophil, and basophil/mast cell types. Other cloned IL 3-dependent cell lines are committed to a single pathway. Studies with Friend spleen focus-forming virus indicate that the first effect in the marrow culture is mediated through a subset of adherent hematopoietic stem cells. Bone marrow culture-derived IL 3-dependent cell lines provide a model with which to study the role of viral genes in the control of differentiation and self-renewal capacity of hematopoietic stem cells.

Animals↗
PubMed1976

The responses of the popliteal lymph node of the sheep to Ross River and Kunjin viruses.

The responses of popliteal lymph nodes of sheep to Ross River virus (RRV) or Kunjin virus (KV) have been studied by monitoring the cell populations, virus titre, antibody titre and plaque-forming cell (PFC) content of the efferent lymph. In a typical primary response to subcutaneous inoculation in the lower hind limb of either RRV or KV, the flow rate of lymph increased slightly. Increased cell concentrations in the lymph following inoculation caused the hourly cell outputs to rise 5.3- to 10.1-fold above pre-challenge levels. High cell outputs were sustained at greater than 3-fold above pre-challenge figures for up to 10 days. The increased number of large, blastic lymphocytes ranged from 4.0- to 14.9-fold; the increase in these cells usually followed the rise in total number of lymphocytes by 24 to 48 h and remained at greater than 3-fold higher levels for up to 12 days. Ross River virus was recovered in the efferent lymph for at least 36 h after inoculation. Sudden disappearance of the virus from the lymph was correlated with the onset of significant haemagglutination-inhibition antibody titres by 3 to 4 days after infection. In the efferent lymph, PFC were detected 5 to 7 days after primary or secondary inoculation of KV or RRV when lymphocytes were incubated with specific haemagglutinin-coated goose erythrocytes and guinea-pig complement. Antibody levels in the lymph were persistent while the occurrence of PFC was transient.

Animals↗