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Biomedical subjects

A Harto

Publications and source records attributed to A Harto.

At least 19 recordsLinked to original sources
PubMed2002

SHP-1 expression in peripheral T cells from patients with Sezary syndrome and in the T cell line HUT-78: implications in JAK3-mediated signaling.

SHP-1 is a key tyrosine phosphatase that acts as a negative regulator of signal transduction in lymphocytes, which has been found down-regulated in several T cell lines derived from human T cell malignancies. The standardization of a sensitive ELISA for the quantification of SHP-1 protein in peripheral T and B lymphocytes has enabled us to quantify the SHP-1 content of freshly isolated T cells from patients with Sezary syndrome and in the Sezary T cell line HUT-78. In all cases, a dramatic decrease in the content of this protein, when compared with the content in healthy volunteer controls, was observed. These results were corroborated when the expression of SHP-1 mRNA was analyzed. In order to study whether there was any correlation between SHP-1 protein expression and tyrosine phosphorylated state of JAK3, the state of phosphorylation of JAK3 was studied in the T cell line HUT-78, and found to be highly phosphorylated. These results suggest that SHP-1 might be involved in maintaining the IL-2R/JAK3 signaling pathway under control and point towards a role of SHP-1 in the pathogenesis of the disease.

Enzyme Induction↗
PubMed2001

An improved data acquisition method for electrical impedance tomography.

Isaacson, Cheney and Seager have demonstrated that simultaneously applying trigonometric patterns of current to a circular electrode array optimizes the sensitivity of EIT to inner structure. We have found that it is less desirable to measure voltage at an electrode that also applies a current due to variable contact impedance. In order to preserve the optimum sensitivity while minimizing the effect of electrode artefacts, we have devised an approach where we sequentially apply a current between each individual electrode and a separate, fixed ground while measuring voltages at all other electrodes for each consecutive current impulse. By adding weighted sums of both the applied currents and corresponding measured voltages from individual passes, we can synthesize trigonometric patterns of any spatial frequency. Since only one of the electrodes in any given acquired data set is used as a source, this approach significantly dilutes the effect of contact impedance on the resulting voltage measurements. We present simulated data showing the equivalency between the synthesized and actual trigonometric excitation patterns. In addition, we report experimental data, both in vitro and in vivo, that show improved results using this data acquisition technique.

Adult↗
PubMed1997

Progressive ascending telangiectasia treated with the 585 nm flashlamp-pumped pulsed dye laser.

BACKGROUND AND OBJECTIVE: Progressive ascending telangiectasia (PAT) is a distinct entity with telangiectatic superficial vessels on lower extremities as its main clinical feature. A relationship with occult infections and response to antibiotic and antifungal drugs have been described, although not all cases can be successfully managed with these therapies. Our objective was to treat a woman with PAT that had failed to respond to systemic antibiotic and antifungal drugs. STUDY DESIGN/PATIENTS AND METHODS: A 44-year-old woman with PAT was treated with the flashlamp-pumped pulsed dye laser at 585 nm, with fluences varying from 7 to 7.25 J/cm2. RESULTS: A successful outcome was obtained with this treatment approach, with no relevant adverse effects except for mild pigmentary changes. CONCLUSIONS: Although ectatic vessels on lower extremities are often resistant to dye laser therapy, superficial thin capillaries like those featuring PAT can be eliminated with the pulsed dye laser at 585 nm. Transient pigmentary changes occur on treated areas but they are expected to disappear in 6 to 12 months after treatment. Laser treatment should be considered in PAT despite the extension and location of the lesions.

Adult↗
PubMed1997

Concurrent cytomegalovirus, M. tuberculosis and M. avium-intracellulare cutaneous infection in an HIV patient.

We report a 25-year-old HIV-positive man with a past medical history of disseminated cytomegalovirus (CMV) infection, who developed cutaneous lesions during a disseminated mycobacterium infection. The histological changes of CMV and acid-fast bacilli were seen on histopathology of the lesions. Cultures were positive for M. tuberculosis and M. avium-intracellulare (MAI). CMV is frequently isolated from HIV patients, but skin involvement is rare. The association of CMV and mycobacteria can occur in cutaneous lesions of AIDS patients, but concurrent cutaneous involvement of CMV, M. tuberculosis, and MAI is unusual. These findings emphasize the polymorphous presentation of infectious disorders in AIDS patients and the need for multiple biopsies and for special stains in such patients.

AIDS-Related Opportunistic Infections↗
PubMed1995

Infrequent expression of protein p53 in epidermotropic variants of cutaneous T-cell lymphoma.

Although diverse types of lymphomas have been examined for immunohistochemical detection of p53 protein, little information is available with regard to p53 protein expression in CTCL. We analyzed cutaneous biopsy specimens of 22 patients with the diagnoses of mycosis fungoides or Sézary syndrome with polyclonal rabbit anti-p53 antiserum CM-1. Staining of neoplastic cells was observed only in two patients with advanced disease. Overexpression of p53 protein does not seem to be a major feature of either mycosis fungoides or Sézary syndrome.

Adult↗
PubMed1995

Plasma levels of 8-methoxypsoralen after bath-PUVA for psoriasis: relationship to disease severity.

Plasma levels of 8-methoxypsoralen (8-MOP) were determined by high-pressure liquid chromatography in 19 patients with psoriasis who were receiving bath-PUVA treatment, at different time points after the psoralen bath. The levels of 8-MOP varied between < 5 ng/ml (lower limit of detection) and 34 ng/ml, and we found a relationship between the plasma psoralen levels and the severity of the disease.

Administration, Cutaneous↗
PubMed1994

Mucocutaneous leishmaniasis and HIV.

Mucocutaneous leishmaniasis is a rare disease in Europe. Relapses after treatment are more frequent than in visceral leishmaniasis. HIV patients infected by Leishmania have frequently visceral involvement, and responses to treatment are poor. Mucocutaneous leishmaniasis in HIV-infected patients has rarely been reported. A patient with centrofacial granuloma was diagnosed as having mucocutaneous leishmaniasis; simultaneously HIV infection was detected. To our knowledge this is the first case acquired in Europe. Intravenous meglumine antimonate 20 mg/kg/day for 28 days was proven to be useful.

AIDS-Related Opportunistic Infections↗
PubMed1993

[Facial angioma and the Sturge-Weber syndrome: a study of 121 cases].

BACKGROUND: We show a study in 121 patients with port-wine stain (PWS) over the areas of trigeminal nerve sensory innervation, and we analyzed the relations between this distribution, sex and the neuro-ocular disease. METHODS: The population included 68 women between 12 months and 73 years (mean, 23 years; standard deviation, 13.7 years) and 53 men between 12 months and 50 years (mean, 18 years; standard deviation, 12 years). Chi-square test and Fisher test were performed in order to assess statistical significance of our results. RESULTS: Facial port-wine stains (PWS) were predominantly distributed over the area of innervation of the maxillary branch (V2) of the trigeminal nerve (88% of patients), either isolated or simultaneously involving ophthalmic (V1) and/or mandibular branch (V3). Of the unilateral PWS (86%), 10% were also extrafacially distributed. However, bilateral PWS (14%) extended extrafacially in 53% of the patients. Moreover, the number of patients with extrafacial PWS was increased among those with PWS over V3 (50%). Glaucoma and epilepsy were present in 23 (17%) and 17 (14%) patients, respectively. In all of them PWS affected V1. Among the 19 patients with bilateral PWS, nine of them developed glaucoma and five, epilepsy. Epilepsy was more frequent in men (26%) than in women (4%). CONCLUSIONS: Statistical analysis showed: 1) the PWS is preferentially distributed over the maxillary branch of the trigeminal nerve, 2) bilateral PWS significantly increases the frequency of cutaneous extrafacial PWS, 3) the presence of PWS over the mandibular branch of trigeminal nerve carries a higher risk of cutaneous extrafacial presence of the angioma, 4) only those patients with PWS over the ophthalmic branch may develop glaucoma and/or epilepsy, 5) extrafacial PWS is associated with a higher frequency of glaucoma, 6) men have a higher risk of developing epilepsy than women.

Adolescent↗
PubMed1992

Interferon-alpha therapy in atopic dermatitis.

Thirteen patients with a severe adult form of atopic dermatitis (AD) received 3.0 x 10(6) IU of recombinant interferon-alpha 2a (rIFN-alpha 2a) 3 times a week. A satisfactory response was obtained in 5 of them. Serum IgE levels in all 13 patients remained unchanged throughout the study. Flu-like symptoms were common, but clinical or laboratory adverse effects were otherwise slight. The moderately beneficial therapeutic effects observed in this study support a possible role for IFN-alpha in controlling immunologic deficiencies in atopic dermatitis.

Adolescent↗
PubMed1990

Vegetating iododerma with underlying systemic diseases: report of three cases.

Three patients with vegetating iododerma as a result of potassium iodide therapy are presented. The first patient had polyarteritis nodosa, the second had monoclonal gammopathy of undertermined significance, and the third had multiple myeloma. Vegetating iododerma probably represents an idiosyncratic response to iodides; patients with polyarteritis nodosa and paraproteinemias may be predisposed.

Aged↗