PubMed Health⌕ Search

Biomedical subjects

A Hezzey

Publications and source records attributed to A Hezzey.

8 recordsLinked to original sources
PubMed1982

Platelet concentrates. Influence of different preparative protocols on the in vitro release reaction.

To determine what effect different preparative protocols and different platelet storage bags have on in vitro release, platelet concentrates were prepared according to two different protocols (group I: 2,200 g for 3.5 min followed by 4,900 g for 5 min; group II: 1,000 g for 9 min and 3,000 g for 20 min). The storage bags used were either a standard polyvinyl chloride (PL-146) or a polyolefin bag (PL-732). The results for platelet concentrates stored in PL-146 showed that while group I had undergone more alpha granule release at 24 and 48 h of storage than had group II, by 72 h both groups showed a similar degree of release. Results for platelets stored in PL-732, however, did not show any significant difference in release between the two protocol groups at any storage time up to 120 h. We conclude that although the preparative protocol used may induce different degrees of platelet release in vitro, the platelet storage bag also has a significant influence on the degree of in vitro damage which occurs during subsequent storage.

Alkenes↗
PubMed1981

Occurrence of the release reaction during preparation and storage of platelet concentrates.

To determine the degree of damage occurring during preparation and storage of platelet concentrates, the percent release of B-thromboglobulin (BTG) and percent leakage of the cytosolic protein lactic dehydrogenase was determined sequentially from phlebotomy to the end of storage for 72 h at 20-24 degrees C. The effect of storage temperature, pH, and radiation was also evaluated. The results showed that during preparation of platelet concentrate a large degree of release was found after resuspension of the platelet button formed after the high-speed centrifugation. During storage the percent BTG release increased from 18.1 to 40.2% (p less than 0.05). The percent release seen during storage at 4 degrees C (72 h) was 19.2%, while that seen for platelets subjected to temperature cycling at 4-37 degrees C was 24.9%. Both of these values were significantly less (p less than 0.05) than that seen for concentrates stored at room temperature. A negative correlation between pH and BTG release was found (r = -0.64). Irradiation to 10,000 rad did not induce the release reaction or lactic dehydrogenase leakage. We conclude that the degree of in vitro platelet release is dependent on the preparative manipulations, and gentler protocols for preparation and storage of platelets should be investigated.

Beta-Globulins↗
PubMed1981

Effect of blood transfusion on in vivo levels of plasma fibronectin.

Fibronectin, an opsonic glycoprotein, is known to bind fibrinogen and fibrin. Microaggregate debris contained in stored bank blood is composed of degenerating platelets, leukocytes, and fibrin strands. The debris ranges in size from 10 to 160 micrometers. This study examined the effect of transfusion of 2 units of stored red blood cells, containing varying amounts of macroaggregate debris, on in vivo levels of fibronectin. Anemic outpatients were selected at random to receive blood transfusion through either a 170 micrometer standard blood filter or one of four microaggregate filters. A sixth group received saline-washed red blood cells. Results showed that the greatest drop in posttransfusion levels of fibronectin (39 microgram/ml) were found in the group that received blood through a 170 micrometer standard filter and thus received the greatest amount of microaggregate debris (p less than 0.05; N = 50). The patients who received the least microaggregate debris, those receiving washed red cells, showed the smallest decrease in posttransfusion levels of fibronectin (9 microgram/ml); this decrease was not significant (p greater than 0.05; N = 50). Patients receiving blood through 20 to 25 micrometer microaggregate blood filters showed a fall in posttransfusion levels of fibronectin (10 to 15 microgram/ml) intermediate between that found for the two groups described above; this decrease was not significant (p greater than 0.05; N = 35). The decrease in posttransfusion levels of fibronectin found after administration of blood through a 40 micrometer microaggregate screen filter (31 microgram/ml) was significantly different from pretransfusion levels (p less than 0.05; N = 20). Data obtained from this study suggest that transfusion of the microaggregate debris contained in 2 units of stored bank blood can lower in vivo levels of fibronectin. We conclude that if maintaining high levels of fibronectin is shown to be of value in the treatment of critically ill patients, removal of microaggregate debris from any blood transfusions required by these patients would be warranted.

Adult↗
PubMedDate not supplied

Microaggregate blood filtration in patients with compromised pulmonary function.

To determine the effect of microaggregate blood filtration on patients with compromised pulmonary function, we divided 50 patients having elective coronary bypass surgery into two groups. One group received all blood transfusions via a 170-micron standard filter (SF). The other group received blood through a 20-micron microaggregate blood filter (MF). Patients were monitored pre- and postoperatively for changes in arterial blood gases and cardiopulmonary function. Mean transfusion volume was seven units (SF) and six units (MF). Preoperatively, patients in both groups showed abnormal lung function with elevated intrapulmonary shunting (Qs/Qt) and alveolar-arterial gradients (A-aO2). Postoperatively, no significant differences (p greater than 0.05) were found between the groups for any of the tests of cardiopulmonary function evaluated. Posttransfusion, none of the patients in either group showed clinical signs of respiratory distress. We conclude that even for patients with some degree of pretransfusion pulmonary dysfunction, use of a microaggregate blood filter for six- to seven- unit transfusions does not provide significant clinical benefit.

Blood Gas Analysis↗
PubMedDate not supplied

Effect of microaggregate blood filtration on granulocyte concentrates in vitro.

To determine the effect of transfusing granulocyte concentrates through microaggregate blood filters, granulocytes prepared with a cell processor were passed through screen and depth microaggregate filters. Pre- and postfiltration evaluations were made of total granulocyte count, levels of muramidase, granulocyte viability, motility, phagocytosis, bactericidal activity, and hydrogen peroxide-forming capacity. Compared to prefiltration levels, a significant (p less than 0.05) decrease in postfiltration granulocyte counts was seen for all the depth filters studied but not for the standard 170 microns (control) or the 40 microns screen filter. For the various tests of granulocyte function evaluated prefiltration, no significant postfiltration differences (p greater than 0.05) were seen for any of the filters studied. Screen microaggregate filters retained only 1 to 3 percent of granulocytes contained in the concentrates, and thus appear satisfactory for use in clinical transfusions. The large percentage of neutrophils retained by the depth filters (20-62%), however, precludes their use for transfusion of granulocyte concentrates.

Blood↗
PubMedDate not supplied

Stability of red cell antigens and plasma coagulation factors stored in new formulation plastic blood containers.

The stability of red blood cell antigens and plasma coagulation factors stored in new plastic blood packs containing either citrate-phosphate-dextrose (CPD) or citrate-phosphate-dextrose-adenine (CPDA-2) was studied. The containers were made of either polyolefin (PL 732TM) plastic without a plasticizer, or polyvinyl chloride with a non-diethylhexyl phthalate (non-DEHP) plasticizer (PL 1240). All studies were done in parallel using standard polyvinyl chloride (PL 146) bags containing DEPH plasticizer as controls. Red blood cell antigen scoring was performed for the A, B, c, D, K, Fya, Lea, Jka, M, and P antigens using cells obtained from both the blood container and tubing segments. Units of fresh frozen plasma and cryoprecipitate were prepared, and age and donor-matched sets of each of these blood components were stored in both test and control plastic containers. The red blood cell antigens showed no decrease in reactivity over 21 (CPD) or 35 (CPDA-2) days of storage in either test plastic compared with controls. For fresh frozen plasma (factors V, VIII, IX) and cryoprecipitate (factor VIII) the factor activity found for the test plastics ranged from 94 to 115 percent of that recorded for the age and donor-matched control plastic. The concentration of fibronectin found in cryoprecipitate stored in the test plastics averaged 90 to 99 percent of the activity found in PL 146 plastic. We conclude that the red blood cell antigens and coagulation factors tested remain stable in the plastic-anticoagulant combinations studied.

Anticoagulants↗
PubMedDate not supplied

Stability of red cell antigens during prolonged storage in citrate-phosphate-dextrose and a new preservative solution.

Red cells anticoagulated with citrate-phosphate-dextrose (CPD) or a commercial preservative solution containing adenine, sodium chloride, and mannitol (ADSOL) and stored in heat-sealed segments of plastic tubing were tested for antigen stability after refrigeration at 4 degrees C for up to 56 days. Reactivity of the A, B, c, D, K, Lea, Fya, M, and P1 antigens was maintained adequately during the storage period in both solutions. Heat-sealed segments containing red cells suspended in either CPD or ADSOL preservation solutions for up to 56 days appear acceptable for use in compatibility testing.

Adenine↗