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Biomedical subjects

A Hinton

Publications and source records attributed to A Hinton.

At least 19 recordsLinked to original sources

Nasal deformity and interpersonal problems.

Several studies in facial plastic surgery have suggested that changes in body image that initiate a positive psychological cycle play an important role in a successful outcome. The change in the patient's self-perception leads to a sense of well-being, rather than there being a direct impact from the changed features. One area in which this change in self-perception may be manifest is in the development and maintenance of interpersonal relationships. Due to the impact of negative body image preoperatively, we hypothesized that patients with nasal deformity may have problems with their interpersonal relationships. This theory was tested using the IIP-32: a short version of the inventory of interpersonal problems. A prospective trial was undertaken of 27 rhinoplasty patients with nasal deformity and 16 septoplasty patients without nasal deformity, the latter group used as a control. The results show that the null hypothesis that the two groups are from the same population on all dimensions of the IIP-32 cannot be rejected (total mean score 0.753). From this data, it seems that patients with nasal deformity have no more difficulties in their interpersonal relationships and, as such, this particular issue cannot be used in support of rhinoplasty surgery.

Adult↗

Assessing psychological morbidity in patients with nasal deformity using the CORE questionnaire.

The aesthetic facial surgeon's evaluation of the patient seeking cosmetic surgery must be thorough. Those with emotional disturbances and overt psychiatric disorders are considered to be poor candidates for elective cosmetic surgery. In patients with nasal deformity, the prevalence of subclinical psychological morbidity and the role of cosmetic surgery in these circumstances are uncertain. This study investigates the use of the CORE questionnaire in assessing the psychological status of patients awaiting rhinoplasty or septorhinoplasty and compares this with the psychological status of patients awaiting septoplasty. A prospective trial of 27 rhinoplasty patients and 16 septoplasty patients revealed that in terms of life functioning, patients with nasal deformity have significantly higher psychological morbidity compared with patients without nasal deformity. However, there was no significant difference in global psychological status. In addition, female patients with nasal deformity are more distressed in terms of general wellbeing than male patients with nasal deformity and there is a tendency for higher global psychological impairment in women. These results may have implications in terms of health care rationing in patients with nasal deformity and preoperative psychological screening.

Adult↗

Carbohydrate-based cocktails that decrease the population of Salmonella and Campylobacter in the crop of broiler chickens subjected to feed withdrawal.

The efficacy of various carbohydrate-based cocktails in reducing the number of enteropathogens in the crops of broilers subjected to feed withdrawal was examined. Market-aged broilers that had been orally challenged with Salmonella typhimurium were provided the cocktails during a 12-h feed withdrawal. After feed withdrawal, the broilers were processed, and their crops were aseptically removed and weighed. Crops were then blended in distilled water, and the pH of the suspensions was measured electronically. Populations of S. typhimurium, Campylobacter, and lactic acid bacteria in the crop suspensions were enumerated. Findings indicated that significantly fewer S. typhimurium and Campylobacter were recovered from the crops of broilers that had been provided cocktails supplemented with sucrose than from the (Key words: crop, carbohydrate, feed crops of broilers provided cocktails supplemented with equal concentrations (wt/vol) of glucose. Furthermore, significantly fewer S. typhimurium were recovered from the crops of broilers provided cocktails supplemented with 2 to 10% sucrose than from the crops of broilers provided water or cocktails that were not supplemented with carbohydrates. The pH of the crop contents of broilers provided carbohydrate cocktails were lower than the pH of the crops of broilers provided water or cocktails that were not supplemented with carbohydrates. Consumption of the cocktails did not produce significant changes in the crop weights. Findings indicate that altering the composition of carbohydrate-based cocktails provided to broilers during feed withdrawal may affect the efficacy of cocktails in reducing the number of enteropathogens recovered from the crops of broilers.

Animal Feed↗

Topical bupivacaine in paediatric day-case tonsillectomy: a prospective randomized controlled trial.

Post-operative pain and delayed oral intake are common reasons for failure of discharge in day-case tonsillectomy. A double blind prospective randomized study was devised to investigate the effectiveness of topical bupivacaine in reducing post-operative pain in paediatric day-case tonsillectomy. Ninety-nine patients aged between three and 16 years were recruited into the study. One group received bupivacaine soaked swabs tightly packed in their tonsillar fossae while the control group received saline-soaked swabs. The bupivacaine group was found to drink (p < 0.001) and eat (p = 0.006) earlier than the control group. The pain scores at one (p < 0.001), three (p < 0.001) and six (p < 0.001) hours post-operatively were also found to be lower in the bupivacaine group than the control group. We conclude that topical bupivacaine has a role in facilitating recovery in day-case tonsillectomy in children.

Adolescent↗

Unheated water in the first tank of a three-tank broiler scalder.

Scalding with unheated water in the first tank of a simulated three-tank scalder was tested to determine whether carcass bacteria, efficiency of feather removal, and cooked breast meat tenderness are affected as compared with carcasses scalded at the same temperature (57 C) in all tanks. This experiment was performed on 3 d using 6-wk-old broilers. On each day, eight birds per treatment were processed. During the first 40-s scalding period, one carcass was placed in approximately 24 C water. The other carcass was placed simultaneously in a scalder unit containing approximately 2,050 L of water at 57 C. Carcasses were then held out of the water for 15 s, after which both were placed for 40 s in opposite ends of the scalder containing water at 57 C. After the second scalding period, both carcasses were again removed from the water for 15 s, followed by another 40 s in the 57 C water. Total scald time was 2 min for each treatment. After picking, carcasses were rinsed with 200 mL of sterile 0.1% peptone water for 1 min. Aerobic bacteria and Escherichia coli were enumerated and incidence of salmonella was determined by standard methods. After rinsing, carcasses were eviscerated by hand and chilled for 30 min in ice slush. All carcasses were scored for the presence of feathers, and the appearance and condition of the skin were noted. Four hours postmortem, breast fillets were removed from carcasses and chilled overnight at 2 C. The next morning, breast fillets were cooked to an internal endpoint temperature of 75 to 80 C. Warner-Bratzler shear values were measured to determine tenderness. No differences were found in numbers of aerobic bacteria and E. coli, incidence of salmonellae, tenderness of cooked breast meat, or number of feathers left on carcasses.

Animals↗

Reduction of Salmonella in the crop of broiler chickens subjected to feed withdrawal.

Broilers were challenged with 10(9) Salmonella typhimurium and then were provided a glucose-based cocktail supplemented with 0 to 15% glucose during feed withdrawal in battery cages or in pens on litter. After feed withdrawal, broilers were processed, and their crops were aseptically removed and weighed. Crops were then stomached in distilled water, and the pH of the suspension was measured electronically. Salmonella typhimurium, Enterobacteriaceae, and lactic acid bacteria in the crop suspensions were enumerated on the appropriate bacteriological medium. Findings indicated that fewer S. typhimurium and other Enterobacteriaceae were recovered from the crops of broilers provided the cocktail supplemented with 7.5% glucose than from the crops of broilers provided either water or cocktails supplemented with lower or higher concentrations of glucose. Inhibition of the growth of S. typhimurium and other Enterobacteriaceae in the crops of broilers provided the cocktail supplemented with 7.5% glucose was generally associated with increased growth of lactic acid bacteria and decreased crop pH. Providing the cocktail to broilers before shipping to processing plants may reduce the number of food-borne pathogens that poultry carry into processing plants.

Animals↗

Physical, chemical, and microbiological changes in the crop of broiler chickens subjected to incremental feed withdrawal.

Trials were conducted to determine the effect of feed withdrawal on the weight, pH, native bacterial flora, and Salmonella typhimurium persistence in crops of broilers. Six-week-old broilers were provided medicated or unmedicated feed and were subjected to feed withdrawal for 0 to 24 h in transportation crates or on litter. After feed withdrawal, broilers were stunned, bled, scalded, and picked. Crops were aseptically removed and weighed. Crops were then blended in 20 mL distilled water, and pH values of the suspensions were measured. Aerobic bacteria, Enterobacteriaceae, S. typhimurium, and lactic acid bacteria in the suspension were enumerated on appropriate bacteriological media. Feed withdrawal produced significant decreases in the crop weights. Crop pH increased by 1.0 unit within 6 h of feed withdrawal. Enterobacteriaceae and S. typhimurium populations generally decreased during the first 12 h of feed withdrawal and remained unchanged or increased during the final 12 h of the 24-h feed withdrawal period. The number of lactic acid bacteria recovered from the crop usually decreased as the duration of feed withdrawal increased. Changes in crop weights, pH, native microflora, or the recovery of S. typhimurium from the crop were similar in broilers subjected to feed withdrawal on litter or in crates and in broilers provided medicated or unmedicated feed before initiating feed withdrawal. Findings indicate that a decrease in the number of lactic acid producing bacteria in the crop and an increase in crop pH may be related to the reduction of anti-Enterobacteriaceae activity in the crop during extended feed withdrawal.

Animals↗

Influence of flooring type during transport and holding on bacteria recovery from broiler carcass rinses before and after defeathering.

Four trials were conducted to determine whether conventional solid or elevated wire mesh flooring, during transport and holding of broilers prior to slaughter, influenced the number of bacteria recovered from feathered and defeathered carcasses. After 4 h off feed, 7-wk-old broilers were placed at commercial density into a modified commercial transport dump-coop on either fiberglass sheeting or 2.54x2.54 cm wire mesh flooring that allowed feces to fall through. Broilers were transported for 1 h and then held for 13 h under a covered shed before processing. Broilers were killed by electrocution, and the vents were plugged to prevent escape of feces. External carcass rinses were obtained twice (from the same carcass) from eight broilers per flooring treatment per trial, before scalding and defeathering and again after defeathering and removal of the head and feet. Greater numbers of total aerobes, coliforms, and Escherichia coli were recovered from feathered carcasses than from defeathered carcasses. Campylobacter count was also less for defeathered than feathered carcasses from the solid flooring treatment but did not significantly decrease following defeathering of carcasses from the wire flooring. The incidence of Campylobacter-positive carcasses was reduced following defeathering for both flooring treatments, but the percentage of Salmonellae-positive carcasses remained constant. Coliform (log10 6.20 vs. 5.63 cfu/mL of rinse) and E. coli (log10 5.93 vs. 5.36) counts in the feathered rinses were significantly higher for the solid flooring compared with wire flooring, respectively. After defeathering, the number of coliforms (log10 3.12) and E. coli (log10 2.91) recovered did not differ between flooring treatments. Aerobic plate count (log10 7.06 and 4.02), Campylobacter count (log10 2.49 and 1.80), and the incidence of Campylobacter-positive (44 and 11%) and Salmonellae-positive (52 and 50%) carcasses for feathered and defeathered rinses, respectively, did not differ between flooring treatments. These results indicate that although broilers transported and held on solid flooring had noticeably dirtier breast feathers and higher coliform and E. coli counts prior to scalding and defeathering, bacteria recovery from external carcass rinses did not differ between the solid and wire flooring treatments after defeathering.

Animals↗

Physical, chemical, and microbiological changes in the ceca of broiler chickens subjected to incremental feed withdrawal.

Trials were conducted to determine the effect of feed withdrawal on the weight, pH, native bacterial flora, and the persistence of Salmonella typhimurium in the ceca of market-age broilers. Broilers were provided medicated or unmedicated feed and then were subjected to feed withdrawal for 0 to 24 h in transportation crates or on litter. After feed withdrawal, broilers were stunned, bled, scalded, and picked. One cecum from each bird was aseptically removed and weighed. The cecum was then blended in 20 mL of distilled water, and the pH of the blended suspension was measured. The number of total aerobes, Enterobacteriaceae, S. typhimurium, and lactic acid bacteria in the suspension were enumerated on the appropriate bacteriological media. Results indicated that up to 24 h of feed withdrawal produced no significant change in cecal weight and that cecal pH varied by up to 0.3 units during feed withdrawal. There were significant increases in the population of Enterobacteriaceae during feed withdrawal in Trials 2 and 3, and there was a significant increase in the population of cecal aerobes in Trial 3. Feed withdrawal produced significant decreases in the population of lactic acid bacteria in all trials, but no significant change in the population of S. typhimurium occurred during feed withdrawal. There were no significant differences in cecal weight, pH, native bacteria populations, or S. typhimurium populations between broilers that were subjected to feed withdrawal on litter or in crates. Findings indicate that feed withdrawal does not always effectively evacuate the contents of the ceca and that the ceca of broilers subjected to feed withdrawal can remain a source of foodborne bacterial pathogens.

Animal Feed↗

Coliform, Escherichia coli, and salmonellae concentrations in a multiple-tank, counterflow poultry scalder.

Scald water samples from a commercial broiler processing plant were tested for coliforms, Escherichia coli, and salmonellae to evaluate the numbers of suspended bacteria in a multiple-tank, counterflow scalder. Water samples were taken from each of three tanks on 8 different days after 6-week-old broilers had been processed for 8 h. Coliforms and E. coli were counted using Petrifilm, and the most probable number (MPN) of salmonellae was determined both in water samples and in rinses of defeathered carcasses that were removed from the processing line immediately after taking the water samples. Mean coliform concentrations in tanks 1, 2, and 3 (the last tank that carcasses pass through before being defeathered) were 3.4, 2.0, and 1.2 log10(CFU/ml), respectively. E. coli concentrations followed the same pattern with means of 3.2, 1.5, and 0.8 in tanks 1, 2, and 3, respectively, with significant differences (P < 0.02) in the concentrations of both coliforms and E. coli between the tanks. Sixteen of 24 scald-water samples were positive for salmonellae with a geometric mean of 10.9 MPN/100 ml in the positive samples. Salmonellae were isolated from seven of eight water samples from both tanks 1 and 2, but in only two of eight water samples from tank 3, the last tank that carcasses pass through. It appears that most bacteria removed from carcasses during scalding are washed off during the early part of scalding.

Animals↗

Use of oleic acid to reduce the population of the bacterial flora of poultry skin.

The effect of oleic acid on native bacterial flora of poultry skin was examined. Skin from commercial broiler carcasses was washed once or twice in solutions of 0, 2, 4, 6, 8, or 10% (wt/vol) oleic acid and rinsed in peptone water. Aerobic bacteria, Enterobacteriaceae, Campylobacter, and enterococci in the rinsates were enumerated. Significantly fewer aerobic bacteria, Enterobacteriaceae, Campylobacter, and enterococci were recovered from rinsates of skin washed in oleic acid than from control samples. Additionally, fewer bacteria were recovered from rinsates of skin washed in higher concentrations of oleic acid than from skin washed in lower concentrations of the fatty acid. In most cases, there was no significant difference in the number of bacteria recovered from rinsates of skin washed once or twice in solutions of oleic acid. Washing skin samples twice in 10% solutions of oleic acid significantly reduced the number of aerobic bacteria, Enterobacteriaceae, Campylobacter, and enterococci that remained attached to the skin. Campylobacter sp., Enterococcus faecalis, and Listeria monocytogenes isolates possessed the least resistance to the antibacterial activity of oleic acid in vitro, while Escherichia coli and Pseudomonas aeruginosa showed higher resistance. Enterobacter cloacae, Staphylococcus lentus, and Salmonella Typhimurium had the greatest resistance to the antibacterial activity of oleic acid. Findings indicate that oleic acid reduces the number of bacteria on the skin of processed broilers and that the fatty acid is bactericidal to several spoilage and pathogenic bacteria associated with poultry.

Animals↗

Research note: in vitro inhibition of Listeria monocytogenes growth by veillonellae cultures grown on tartrate media.

Veillonellae cultures were grown on agar media supplemented with tartrate and examined for inhibitory effects on the growth of Listeria monocytogenes. Veillonellae cultures grown on media supplemented with 0 or 50 mmol l-1 of tartrate did not inhibit the growth of L. monocytogenes; however, veillonellae grown on media supplemented with 100, 150 or 200 mmol l-1 of tartrate did inhibit the growth of L. monocytogenes. The inhibition of the growth of L. monocytogenes by the veillonellae was correlated with the increased production of acetate and propionate from tartrate by veillonellae and with the reduction of the pH of the media by L. monocytogenes.

Bacteriological Techniques↗

The incidence of bacteria in middle ear effusions.

Otitis media with effusion (OME) is a common condition in paediatric ENT practice. Whilst surgical management is in many cases the mainstay of treatment for resistant OME, the use of antibiotics has been advocated by some authorities. Over one quarter of middle ear effusions analysed in this study contained potentially pathogenic bacteria. Antibiotics may be of value in the treatment of OME in these children.

Anti-Bacterial Agents↗

Inhibition of Salmonella typhimurium by the products of tartrate metabolism by a Veillonella species.

The inhibition of the growth of Salmonella typhimurium by a Veillonella species grown on media supplemented with tartrate was examined. Growth of Salmonella typhimurium was not inhibited by the concentrations of products metabolized by Veillonella cultures on media supplemented with 0 or 50 mmol l-1 of tartrate, but was inhibited on media supplemented with 100 or 150 mmol l-1 of tartrate. Inhibition of Salm. typhimurium was correlated with the increased production of acetate and propionate from tartrate by the Veillonella species.

Acetates↗

Sensitivity of Escherichia coli O157:H7 strain 932 to selected anticoccidial drugs in broiler chicks.

The ability of selected anticoccidial drugs to inhibit the colonization of day-old male broiler chicks (Cornish Rocks) by Escherichia coli O157:H7, strain 932 was examined. Chicks were challenged with 1.8 x 10(9) E. coli O157:H7 on Day 1, and fed diets supplemented with three selected anticoccidial drugs; monensin, nicarbazin, or robenidine. The cecal and colon fecal contents of the chicks were removed on Day 7, 14, and 21 postinoculation and examined for the concentration of E. coli O157: H7 per gram of contents. Monensin effectively reduced cecal and colon colonization of the chicks by E. coli O157:H7. By Day 7, there were significant reductions in the bacterial population of the cecal contents of chicks receiving the monensin-medicated feed, and by Day 21 no E. coli O157:H7 was recovered from the cecal and colon contents. The bacterial counts in the colon contents of the nicarbazin- and robenidine-medicated and unmedicated chicks were significantly higher than the monensin-treated chicks. Bacterial populations in the colon contents were high only when there were high bacterial concentrations in the cecal contents.

Animals↗

Hypoparathyroidism counteracts risk factors for osteoporosis.

Although increased bone density has been reported in patients with hypoparathyroidism, it is not known whether hypoparathyroidism can overcome the influence of risk factors for osteoporosis and whether the increased bone density is uniform throughout the entire skeleton or greater in certain regions depending on the bone composition and location. In the current study, bone density was measured in patients with postsurgical hypoparathyroidism and risk factors for osteoporosis. Bone mineral density was determined in eight patients with postsurgical hypoparathyroidism, one with idiopathic hypoparathyroidism, and two with pseudohypoparathyroidism in eight different areas of the skeleton using well established methods: single photon absorptiometry of the radius, dual energy x-ray absorptiometry of the spine, hip, and the whole skeleton, and quantitative computed tomography of the spine. Risk factors for osteoporosis were documented in each subject. The data showed that despite the presence of 1-4 risk factors for osteoporosis, patients with postsurgical hypoparathyroidism had bone mineral density above the normal mean in most locations. The locations with the highest increment were the Ward's triangle and the trochanter area of the proximal femur (dual energy x-ray absorptiometry) (Ward's: Z score + 1.59 +/- 0.57, P < 0.03; trochanter 1.31 +/- 0.42, P < 0.02). The elevation of bone density was not observed in one patient with idiopathic hypoparathyroidism, and variable results were observed in two patients with pseudohypoparathyroidism. Based on these findings, in postsurgical hypoparathyroidism, the bone mineral density is above the normal mean despite the presence of risk factors for osteoporosis, and both cortical and trabecular bone are affected.

Adolescent↗

Otorhinolaryngology.

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Computer-Assisted Instruction↗