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A I Agbata

Publications and source records attributed to A I Agbata.

4 recordsLinked to original sources
PubMed1980

The E-rosette augmenting factor (E-RAF): aspects of clinical application.

It has recently been demonstrated that antigen- or mitogen-stimulated normal lymphocytes release a soluble E-rosette augmenting factor (E-RAF) which enhances E-rosette formation. Using this enhanced E-rosette formation as an immunologic marker, we studied lymphocytes from twelve normal volunteers, ten patients with chronic mucocutaneous candidiasis and one male patient with severe combined immune deficiency (SCID). These studies confirmed the observation that lymphocytes from normal donors respond to mitogen or specific antigen stimulation by release of E-RAF and increased E-rosette formation. Lymphocytes from the SCID patient were unable to release the soluble factor following mitogenic stimulation and increased E-rosette formation did not occur. Furthermore, our data indicated that short-term incubation (18 hr) of normal lymphocytes in mitogen (PHA or Con A)-derived supernatant fluids containing the soluble factor may offer a reliable and highly reproducible assay technique for total E-rosettes.

Antigens↗
PubMed1979

Release of E-rosette augmenting factor (E-RAF) after stimulation of human leukocytes with mitogens or antigens.

Stimulation of human peripheral blood leukocytes (HPBL) with mitogens such as phytohemagglutinin or concanavalin A increases the total number of lymphocytes that form rosettes with sheep erythrocytes. A similar effect is seen when HPBL from skin test-positive, but not skin test-negative, donors are stimulated by a specific antigen. It was also found that the culture fluids from mitogen-stimulated lymphocytes contained a substance that significantly increased the percentage of E-rosette forming cells (85 to 95%) over that of control culture fluids (40%). A similar phenomenon was also observed with supernatant fluids derived from antigenic stimulation of cells from skin test-positive donors, but not from skin test-negative subjects. The factor that produces this effect has been designated E-rosette augmenting factor (E-RAF). It is nondialyzable; it appears in the supernatants of antigen or mitogen-stimulated cells within 12 hr; and its production is not blocked by mitomycin C. It is produced by cells that do not adhere to glass wool columns and by mitogen-stimulated thymocytes. Sephadex G-100 chromatography showed that mitogen-induced E-RAF eluted from the column in advance of antigen-induced E-RAF. E-RAF has many properties that are characteristic of lymphokines.

Antigens↗