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A I Nesterov

Publications and source records attributed to A I Nesterov.

63 records · Page 4Linked to original sources
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[Numerical analysis of the protein electrophoregrams of obligate methanotrophic bacteria].

The protein spectra for 70 strains of obligate methanotrophic bacteria were studied using electrophoresis in polyacrylamide gel with sodium dodecyl sulphate. The protein spectra of Methylomonas methanica 12 and Methylosinus trichosporium 44 did not change in the course of the culture growth. Basing on the data obtained, the similarity coefficients were calculated for these strains. The numerical analysis of the similarity coefficients was done, and a dendrogram presenting the phylogenetic relationships between these bacterial strains, species, genera and groups was constructed. The coefficients of similarity between the strains of one species varied within the range of 78 to 100%. The similarity coefficients of the species and genera varied from 36 to 38%, respectively. The data suggest the existence of 5 independent genera of obligate methanotrophic bacteria: Methylomonas, Methylobacter, Methylococcus, Methylosinus and Methylocystis.

Electrophoresis, Polyacrylamide Gel↗
PubMedDate not supplied

[Comparative study of the growth parameters of methanotropic bacteria].

Growth parameters and their interrelationship were determined for two mixed and seven pure cultures of methanotrophic bacteria. The specific growth rate, the rate of methane assimilation, and the economic coefficient changed significantly in the course of cultivation of the methanotrophic cultures in the periodic regime, the changes being of the extremem character. Therefore, mean values of the growth parameters of these cultures determined under identical conditions were compared. These parameters were found to be dependent on the taxonomy of the cultures and on the conditions of their growth. No considerable differences had been found in the growth parameters between mixed and pure methanotrophous cultures. Portions of the carbon of methane expended for synthesis of the biomass, carbon dioxide, and exometabolites was different among methanotrophic cultures belonging to different genera.

Bacteria↗