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Biomedical subjects

A I Zhankov

Publications and source records attributed to A I Zhankov.

10 recordsLinked to original sources
PubMed2003

Characterization of a siberian virus isolated from a patient with progressive chronic tick-borne encephalitis.

A strain of Tick-borne encephalitis virus designated Zausaev (Za) was isolated in Siberia from a patient who died of a progressive (2-year) form of tick-borne encephalitis 10 years after being bitten by a tick. The complete genomic sequence of this virus was determined, and an attempt was made to correlate the sequence with the biological characteristics of the virus. Phylogenetic analysis demonstrated that this virus belongs to the Siberian subtype of Tick-borne encephalitis virus. Comparison of Za virus with two related viruses, a Far Eastern isolate, Sofjin, and a Siberian isolate, Vasilchenko, revealed differences among the three viruses in pathogenicity for Syrian hamsters, cytopathogenicity for PS cells, plaque morphology, and the electrophoretic profiles of virus-specific nonstructural proteins. Comparative amino acid alignments revealed 10 individual amino acid substitutions in the Za virus polyprotein sequence that were different from those of other tick-borne flaviviruses. Notably, the dimeric form of the Za virus NS1 protein migrated in polyacrylamide gels as a heterogeneous group of molecules with a significantly higher electrophoretic mobility than those of the Sofjin and Vasilchenko viruses. Two amino acid substitutions, T(277)-->V and E(279)-->G, within the NS1 dimerization domain are probably responsible for the altered oligomerization of Za virus NS1. These studies suggest that the patient from whom Za virus was isolated died due to increased pathogenicity of the latent virus following spontaneous mutagenesis.

3' Untranslated Regions↗
PubMedDate not supplied

[Comparative study of the oligopeptide maps of virus-specific proteins of the viruses of the tick-borne encephalitis complex].

A comparative analysis of tryptic hydrolysates of virus-specific proteins NV5, V3 and NV3 of the tick-borne encephalitis complex viruses showed proteins NV5 to have considerable regions of similar amino acid sequences, V3 proteins to have significantly differing primary structures, and NV3 proteins to have different amino acid sequences.

Amino Acid Sequence↗
PubMedDate not supplied

[Differences in the electrophoretic mobility of the low-molecular virus-specific proteins of viruses of the tick-borne encephalitis complex].

Analysis of low-molecular virus-specific proteins of all the known members of the tick-borne encephalitis (TBE) complex viruses: TBE (2 strains), Langat, louping-ill, Negishi, Kyasanur Forest disease, Omsk hemorrhagic fever (2 strains), and Powassan, was performed. The cells infected with the above viruses were found to contain low-molecular virus-specific NVX (with the exception of Powassan, Omsk hemorrhagic fever, and Kyasanur Forest disease viruses), NV21/2, NV2, NV11/2, and NV1 proteins. These proteins (except NV1) differ in the electrophoretic mobility in the viruses under study and in TBE strains No. 256 and Sophyin. Thus, electrophoresis may demonstrate differences in the characteristics of proteins of different TBE complex viruses and strains of one virus.

Electrophoresis, Polyacrylamide Gel↗
PubMedDate not supplied

[Comparative analysis of the electrophoretic mobility of the high-molecular virus-specific proteins of flaviviruses].

A comparative study of high molecular virus-specific proteins in cells infected with flaviviruses from 5 serological subgroups was carried out. Proteins NV5, NV4, and V3 were found to have similar electrophoretic mobility in viruses of the tick-borne encephalitis (TBE) complex with the exception of Powassan virus protein. Proteins NV5 and NV4 of mosquito-borne flaviviruses differ in electrophoretic mobility both from the corresponding proteins of the TBE complex viruses and from each other. Protein NV4 1/2 is formed only in cells infected with TBE complex viruses. Varying electrophoretic mobility of protein NV3 in different flaviviruses attests to its virus-specific nature.

Animals↗
PubMedDate not supplied

[Heterogeneity of virus-specific flavivirus proteins].

The polyacrylamide gel analysis of large intracellular virus-specific proteins NV5, NV4, and the intracellular form of structural protein V3 established differences in the electrophoretic mobility of each of these proteins formed in cells infected with tick-borne encephalitis, Powassan, Langat, and West Nile viruses. It is assumed that these differences in the electrophoretic mobility of NV5, NV4 proteins, and the intracellular form of V3 protein reflect the differences in the primary structure of each of these proteins in the viruses examined.

Electrophoresis, Polyacrylamide Gel↗
PubMedDate not supplied

[Electron microscopic study of swine embryonic kidney cells infected with the tick-borne encephalitis virus].

Electron microscopic studies of morphological lesions in pig embryo kidney cells (PEK) infected with tick-borne encephalitis (TBE) virus as well as morphology and features of TBE morphogenesis after treatment with actinomycin D, cycloheximide and hypertonic NaC1 concentrations in the medium were carried out. Most marked morphological lesions were observed in the cells after combined effect of high NaC1 concentrations in the medium and inhibitors of protein synthesis. After all kinds of treatment, smooth-contour membrane structures were observed in TBE-infected cells. Their number increased considerably with increasing ionic strength of the medium and subsequent return to normal accompanied by treatment with actinomycin D and cycloheximide. No "budding" particles were found in any case and after any treatment. Features of TBE virus morphogenesis are discussed.

Animals↗
PubMedDate not supplied

[Effect of actinomycin D, cycloheximide and hypertonic concentrations of NaCl on the reproduction of the tick-borne encephalitis virus].

Synthesis of virus-specific polypeptides occurs in pig embryo kidney cells infected with tick-borne encephalitis virus and treated with actinomycin D, cycloheximide, and hypertonic concentrations of NaCl in various combinations. Despite the fact that virus-specific matrix RNAs function under such conditions, production of infectious virus particles is inhibited considerably. Virus yield is greatly decreased by treatment with actinomycin D alone and by combined effect of actinomycin D and cycloheximide. Sodium chloride and combined treatment of the infected cells with cycloheximide and NaCl do not affect the intact virus production. The mechanism of the inhibiting effect of actinomycin D on production of infectious tick-borne encephalitis virus is assumed to consist in the impairment of the synthesis of virus-specific ribonucleic acid and/or in marked reduction of its infectivity. Actinomycin D may possibly inhibit somehow the morphogenesis of intact virions of tick-borne encephalitis virus.

Animals↗
PubMedDate not supplied

[Characteristics of a low-molecular nonvirion ("soluble") antigen from the tick-borne encephalitis virus].

Pig embryo kidney and BHK-21 cells infected with tick-borne encephalitis virus synthesize a nonvirion antigen differing in its immunological properties from the virion antigens. The antigen has a high thermostability. According to the results of ultrafiltration and gel filtration, its molecular weight is approximately 70-100 kilodaltons. Electrophoretic analysis in polyacrylamide gel showed the molecular weight of a polypeptide isolated from the precipitation band formed by this low molecular antigen to differ from molecular weights of virion and high molecular virus-specific nonvirion proteins and to be about 20 kilodaltons. This polypeptide is assumed to be a subunit of the analyzed nonvirion low molecular antigen.

Antigens, Viral↗