PubMed Health⌕ Search

Biomedical subjects

A Inoue

Publications and source records attributed to A Inoue.

At least 577 records · Page 32Linked to original sources
PubMed1980

A comparative study of histology in Perthes' disease and idiopathic avascular necrosis of the femoral head in adults (IANF).

Thirty-seven femoral heads in idiopathic avascular necrosis of adults and fifty-seven biopsy specimens of the capital nucleus of Perthes' disease were studied histologically. Although the early pathological changes in these two diseases are very similar, in the intermediate and late (advanced) stages they gradually take differing courses. Although the initiating causes in both diseases are still obscure both are probably produced by repeated episodes of infarction of the femoral head.

Biopsy↗
PubMed1980

Anterior transfer of the toe flexors for equinovarus deformity of the foot.

Anterior transfer of the long toe flexors was carried out for the treatment of spastic equinovarus foot deformity in both adults and children. Adults included those with hemiplegia subsequent to a stroke, spastic hemiplegia due to cerebral palsy and spastic spinal paraplegia. Most of the children had cerebral palsy. The transfer was indicated for an equinovarus foot with persistent activity of the toe flexors, which produced curling of the toes in the swing phase of the gait or a fixed hammer toe deformity. Fifty six patients were followed up for more than four years. In all cases correction of the equinovarus deformity was achieved and maintained. With satisfactory correction stability of the ankle improved, postural abnormalities during gait decreased and bracing was not required. This study demonstrates the advantage of the long toe flexors for muscle transfer in these patients. The length of tendon available permitted easy transfer to the metatarsal. The defunctioning of the spastic muscles allowed gait improvement and function of the tibialis posterior and tibialis anterior was preserved.

Adolescent↗
PubMed1980

[Experience of peripheral osteosynthesis using single crystal alumina ceramics screws (author's transl)].

As a new kind of material for peripheral osteosynthesis we have developed the single crystal alumina ceramics screws (artificial sapphire screws) taking under consideration of the properties of alumina ceramics, and we have tested them experimentally in animals, and used them clinically too. The results of the mechanical tests and animal experiments were excellent. In the clinical follow-up studies the perfect bone unions has been recognized roentgenologically. The sapphire screws have, sofar, proved to be harmless also as anchor for bone cement. Our experience with our sapphire screws has opened the way for the clinical application of this material instead of metal screws.

Adult↗
PubMed1980

Reaction mechanism of Mn2+-ATPase of acto-H-meromyosin in 0.1 M KCl at 5 degrees C: evidence for the Lymn-Taylor mechanism.

The rate constants of a series of elementary steps in the H-meromyosin (HMM) Mn2+-ATPase [EC 3.6.1.3] and acto-HMM Mn2+-ATPase reactions were determined in 0.1 M KCl at 5 degrees C. We found that the rate-limiting step in the HMM Mn2+-ATPase reaction was the liberation of ADP from HMM.ADP, of which the rate constant was estimated to be 0.17 s-1. All the results obtained with the acto-HMM Mn2+-ATPase reaction could be quantitatively explained by a modified Lymn-Taylor mechanism (see Fig. 15). The second-order rate constant for the dissociation of acto-HMM induced by ATP was 3.0 X 10(5) M-1.s-1, and that for the Pi burst in the acto-HMM ATPase reaction was 1.7 X 10(5) M-1.s-1. The second-order rate constant for the binding of HMM.ADP with F-actin was 0.25 s-1.mg-1.ml, and the rate-limiting step in the acto-HMM Mn2+-ATPase reaction was the conversion of HMMPADP into HMM.ADP plus Pi, when the F-actin concentration was high.

Actins↗
PubMed1980

Preferential phosphorylation of basic non-histone proteins by nuclear protein kinase NII from rat liver.

Rat liver nuclear protein kinase NII, which is independent of cyclic nucleotides, phosphorylated both the acidic protein casein and the basic histone preparation, soluble in 0.25 M HCl, which was extracted from the cell nuclei. When the isolated nuclei were incubated with [32P]ATP in the presence of protein kinase NII, more than 95% of the 32P-labelled proteins was recovered in the 0.25 M HCl extract. In order to analyze the substrate proteins of protein kinase NII, a histone preparation free from the endogenous protein kinase activities was used as substrate. The results demonstrated that histones were not phosphorylated, except for a faint 32P incorporation into the H3 fraction. Instead, multiple non-histone proteins were highly phosphorylated, which were present in a minor quantity in the histone preparation. The molecular weights of the main phosphorylated proteins were 72000, 68000, 56000, 47000, 46000, 43000, 38000 and 32000. Isoelectric focusing demonstrated the basic nature of the phosphorylated proteins, and as much as 72% of 32P radioactivity was distributed in the pH region higher than 7.0. Furthermore, many proteins phosphorylated in the isolated nuclei with added protein kinase NII were also found to be basic non-histone proteins. These results indicate that protein kinase NII preferentially phosphorylates in vitro a set of nuclear basic non-histone proteins.

Animals↗
PubMed1980

Mesothelioma with lung cancer complicating asbestosis.

The patient had been employed in an asbestos factory for four years from the age of 16. Five years ago, she complained of sputum and cough, and she was treated for chronic bronchitis. From March, 1977, when she was 53 years old, hydrothorax and ascites increased, her weight decreased and she was hospitalized for cachexia. The clinical diagnosis of malignant diffuse mesothelioma was made on the presence of atypical cells in the effusions. Atypical cells showed a positive colloidal iron staining test and positive hyaluronidase digestion test. Asbestos bodies were found in the sputum. The patient died on February 1978. Postmortem confirmed asbestosia and mesothelioma which was scattered over the pleura, pericardial sac, diaphragma, peritoneum and pancreas. In addition, bronchiolo-alveolar cell type lung cancer was found localized in the lower lobe of the left lung. The electron beam diffraction disclosed the asbestos as amosite (brown asbestos).

Asbestosis↗
PubMed1979

Phosphorlyation of H1 and H5 histones by cyclic AMP-dependent protein kinase reduces DNA binding.

Phosphorylation of H1 histones by cyclic AMP-dependent protein kinase may be an important transcriptional control mechanism. We have used affinity chromatography to examine the effect of phosphorylation by this enzyme on the DNA-binding properties of calf thymus H1 histones and two highly basic H1 homologues from condensed and transcriptionally silent nuclei: duck erythrocyte H5 and Strongylocentrotus purpuratus sperm H1. Without in vitro phosphorylation, all three histones were eluted from native DNA-Sephadex G-25 columns at salt concentrations which closely resembled those required to extract these histones from nuclei or chromatin. When a small portion of radioactively phosphorylated histone was chromatographed with untreated carrier histone, the phosphorylated species was consistently eluted from the DNA column at slightly lower salt concentrations than the main histone peak. Rechromatography experiments showed that in vitro phosphorylation of H1 can shift its elution position to lower salt concentrations.

Amino Acids↗