Novel flow injection methods for drug-receptor interaction studies, based on probing cell metabolism.
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Animals↗
Biomedical subjects
A Ivaska
Publications and source records attributed to A Ivaska.
Bioligand interaction assay by flow injection absorptiometry.
A novel technique for the study of bioligand interactions based on combining flow injection on renewable surfaces with UV-visible absorptiometry is introduced. The concept is proven by monitoring the binding of various proteins to protein G and the binding of various insulin analogs to a monoclonal anti-insulin antibody, thus providing a comparison with the performance of surface plasmon resonance (SPR)-based techniques. The advantages of the bioligand interaction flow injection absorptiometry approach are speed, low cost, no need for regeneration of solid substrate, and spectral resolution not available with SPR sensing. It is believed that this technique will have an impact on the entire biosensor field since it allows simultaneous monitoring of labeled as well as nonlabeled species in real time over a wide spectral range with high sensitivity.
Adsorption↗
An electrochemical and multispectroscopic study of corrosion of Ag-Pd-Cu-Au alloys.
Corrosion of a multi-phase Ag-Pd-Cu-Au-based commercial dental casting alloy and a Cu-Pd-rich and Ag-rich single-phase alloy was studied by open-circuit potential measurements, atomic absorption spectrometry, and electron spectroscopy. The alloys were immersed in an artificial saliva solution for 24 hr while the open-circuit potentials of the alloys were measured. The potentials were found to stabilize at certain levels after a steep rise during the first hours of the experiment. Cu was found to dissolve considerably from the Cu-Pd-rich alloy, with simultaneous enrichment of Pd in the surface layer of the alloy. Ag dissolved slightly from the Ag-rich alloy, but both Cu and Ag were found to dissolve from the multi-phase alloy. Neither Pd nor Au dissolved from any of the alloys studied.
Chemical Phenomena↗