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A J Amortegui

Publications and source records attributed to A J Amortegui.

At least 37 records · Page 2Linked to original sources
PubMed1986

Changes in the frequency of genital herpes recurrences as a function of time.

To obtain objective information regarding changes in the frequency of recurrent genital Herpes simplex infections, the data from two consecutive pregnancies in 22 women with culture-proved genital Herpes simplex infections were reviewed. The pregnancies studied were separated by a mean of 2.0 years. When only culture-proved recurrences were considered, nine women had fewer recurrences in their second pregnancy than in their first, four had more recurrences in their second than in their first, and nine had the same number of recurrences in both pregnancies. The mean interval between culture-proved recurrences was 58.5 +/- 36.1 (SD) days in first pregnancies and 51.7 +/- 28.6 days in second pregnancies. Mean duration of viral shedding during 14 recurrences in first pregnancies was 4.6 +/- 3.4 days, and 3.2 +/- 2.2 days in 14 recurrences in second pregnancies (differences not significant by Mann-Whitney). Cervical Herpes simplex shedding in asymptomatic women occurred in four of 200 (2.0%) of first pregnancy cultures and zero of 167 second pregnancy cultures (NS). During culture-positive recurrent vulvar infections, 18 of 55 (32.7%) cervical cultures in first pregnancies were positive compared with four of 50 (8%) cervical cultures in second pregnancies (P less than .025). Route of delivery was very similar in the first and second pregnancies with vaginal delivery in 63.6% of first pregnancies and 72.7% of second pregnancies. Overall there was no appreciable difference in the frequency or severity of recurrent genital Herpes simplex infections over time, but more data are needed.

Cervix Uteri↗
PubMed1985

Enzyme immunoassay for detection of Chlamydia trachomatis from the cervix.

An enzyme immunoassay for the detection of Chlamydia trachomatis antigens was compared with isolation of the organism in cycloheximide-treated McCoy cells using duplicate cervical swab specimens from 514 women. Chlamydia trachomatis was isolated after subculturing from 45 of 514 (8.8%) specimens, while the enzyme immunoassay, Chlamydiazyme detected chlamydial antigens in 37 of 45 (82.2%) of those women. Chlamydiazyme was as sensitive as primary isolation for detecting total infections. The specificity of Chlamydiazyme was 98.1%. The predictive values of a positive and negative Chlamydiazyme test compared with isolation after subculturing was 80.4 and 98.3%, respectively. Overall, 497 of 514 (96.7%) samples were identified correctly by Chlamydiazyme. Thus, Chlamydiazyme is a simple and rapid test that eliminates many of the problems encountered by isolation procedures.

Adolescent↗
PubMed1985

A nonculture test for identification of Chlamydia trachomatis.

A clinical trial compared Chlamydiazyme (Abbott Laboratories, North Chicago, Illinois), an enzyme immunoassay being developed for the detection of Chlamydia trachomatis antigens, with isolation of the organism in cycloheximide-treated McCoy cells. Duplicate cervical swab specimens were obtained from 209 women undergoing abortion. C. trachomatis was isolated after subculturing from 18 of them (8.6%). Chlamydial antigens were found with Chlamydiazyme in 13 (72.2%) of the 18. The same number was detected with primary isolation. The specificity of Chlamydiazyme was 98.4%. Overall, 201 of 209 samples (96.2%) were identified correctly with Chlamydiazyme as compared to isolation after subculturing. Therefore, Chlamydiazyme could be used instead of primary isolation in this population.

Adolescent↗
PubMed1984

The effect of chemical intravaginal contraceptives and Betadine on Ureaplasma urealyticum.

The purpose of this study was to find a barrier contraceptive agent capable of controlling infections and sexual transmission of Ureaplasma urealyticum from the female genital tract, especially to help reduce nongonococcal urethritis in males caused by this organism. Therefore, the in vitro antimicrobial activity of six intravaginal contraceptives and BetadineR against the eight serotypes of the organism was investigated. The results indicate that some of these contraceptives produce partial inhibition of the Ureaplasma at low dilutions, while BetadineR produces a ureaplasmacidal effect up to dilutions of 1:64. These effects appear not to be due primarily to the pH of these agents. Thus, some of these agents may have a potential role in controlling transmission of Ureaplasma urealyticum.

Contraceptive Agents, Female↗
PubMed1984

Rapid detection of herpes simplex virus using a combination of human fibroblast cell cultures and peroxidase-antiperoxidase staining.

A method for rapid detection of Herpes simplex virus (HSV), using a combination of human fibroblast cell cultures with peroxidase-antiperoxidase (HF-PAP) staining after 18-24 hours of incubation was developed. The sensitivity of the HF-PAP, compared with viral isolation using primary rabbit kidney and human fibroblast cells, was 88.4% with 20.7% of clinical Herpes isolates detected by HF-PAP before any signs of cytopathic effect (CPE) of HSV were present. The specificity of the new procedure was 98.8%, and overall 739 of 761 specimens (97.1%) were identified correctly by HF-PAP. This system can be used to confirm preliminary or questionable CPE. All of the specimens that exhibited possible CPE at 18-24 hours and from which the virus eventually was isolated were also positive by HF-PAP. However, this method was less sensitive for specimens with smaller amounts of virus. Thus, this method provides a rapid and improved, although not foolproof, means of HSV detection.

Cells, Cultured↗
PubMed1984

A cluster of neonatal herpes simplex infections without mucocutaneous manifestations.

Three cases of neonatal disseminated herpes simplex virus (HSV) infection are reported. They were all due to different strains of HSV-1, according to restriction endonuclease studies, and they represent the first cluster of neonatal-HSV infection at Magee-Womens Hospital. The neonatal symptoms occurred early, suggesting intrauterine infection. None of the babies had mucocutaneous lesions, and the mothers were asymptomatic and had no history of previous genital HSV infection.

Cross Infection↗
PubMed1981

Intramuscular angiomatosis of pelvic wall complicating pregnancy. Clinical, histological, and ultrastructural observations.

This is the first report of intramuscular angiomatosis involving the right bulbocavernous muscle in a pregnant woman, who underwent a cesarean section because of the risk of dystocia. The tumor was formed by well- and poorly differentiated vessels infiltrating the skeletal muscle fibers, which showed various degrees of degeneration. On electron microscopy, the neoplastic vessels were formed by large endothelial cells rich in organelles, limiting a luminal space and surrounded by a multilayered basal lamina. Pericytes were usually present around the endothelial layers. The neoplastic vascular structures seemed to grow by a process of gemmation. Our observations confirm the notion that this kind of benign neoplasm may pursue a rapidly growing course and presents a disturbing histological appearance which requires proper interpretation and precise diagnosis.

Adult↗
PubMed1979

Diffuse vaginal adenosis associated with imperforate hymen.

A case of imperforate hymen associated with adenosis of the proximal surface of the obstructing membrane and of the vaginal wall is reported. The histogenesis of vaginal adenosis coexisting with congenital obstructive lesions is discussed, and the importance of careful follow-up of the patients with this type of anomaly is emphasized.

Adolescent↗
PubMed1977

Short rib-polydactyly syndrome type 3.

Three infants died perinatally of asphyxia due to thoracic narrowing. Their uniform radiographic findings are different from previously described and recognized chondrodysplasias and possibly represent a distinct syndrome. Three identical or very similar cases are found in the literature.

Bone Diseases, Developmental↗
PubMed1976

Postnatal effects of chemically induced intrauterine growth retardation on some hematological values in the rat.

The effects of artificially induced intrauterine growth retardation on hematological values of the rat are manifest only temporarily in the red blood cell number, the amount of hemoglobin, and the 2,3-DPG. These three elements reached normal levels after the fifth day of postnatal life. We believe that this temporary effect may be the consequence of the short-term effect of hydroxyurea (the drug used to produce intrauterine growth retardation) upon DNA synthesis in the hematopoietic system. The animals compensate for the low red cell number and low hemoglobin by raising the levels of 2,3-DPG brought about by the temporary anoxia.

Animals↗