PubMed Health⌕ Search

Biomedical subjects

A J Driesel

Publications and source records attributed to A J Driesel.

31 records · Page 2Linked to original sources
PubMed1985

[Possible common partial antigens in human Ig allotype structure and ubiquitous bacteria, studied with the example of Escherichia coli].

As can be learned from the literature, bovine serum may contain antibodies directed against human immunoglobulin allotypes. This gave rise to the question of what the origin of those antibodies is. We tested bacteria (E. coli) by means of the haemagglutination inhibition assay, which is used to type either Gm or Km factors. Anti-G1m(2) and anti-G3m(10)-specific antibodies were inhibited by the bacteria in a clear-cut manner, as was anti-Km(1), albeit less significantly. In contrast, the bacteria tested almost totally failed to inhibit anti-G3m(21) serum. The results lead to the assumption that E. coli may carry both Gm- and Km-like antigenic structures, which are presumably the antigenic material leading to immunization of cattle. Furthermore, new attention is drawn to a mechanism for immunization which is discussed regarding the genesis of either AB0 isoagglutinins in man or other "naturally occurring" antibodies.

Antibodies↗
PubMed1985

DNA polymorphism of the major histocompatibility class I genes and their association with serologically defined haplotypes.

DNA of unrelated persons as well as members of families that were totally or partially homozygous or completely heterozygous on the loci of the major histocompatibility class I genes has been isolated from peripheral blood lymphocytes and blot hybridized with the class I pseudogene pHLA 12.4 probe. The autoradiographic DNA patterns were discussed and compared with well-defined serological features. Positive associations with serologically typed alleles had been demonstrated for HLA-A1,11; -A2; -A3; -B7; -B14; -B35; -Bw41; and -Cw5.

Alleles↗
PubMed1984

Hereditary persistence of fetal haemoglobin (HPFH) in conjunction with a chromosomal translocation involving the haemoglobin beta locus.

An HPFH syndrome was found in a woman and her daughter who also carry a 'balanced' cyclic translocation of chromosome segments involving four chromosomes, with one break point located in the region of the Hb beta locus. This HPFH is characterized by 5% and 8% Hb F in peripheral blood, uneven distribution of Hb F in the red cells, and a G gamma/G gamma + A gamma ratio of 0.4. The mapping of the non alpha gene cluster shows no detectable deletion in the entire gamma-delta-beta-globin gene region.

Adult↗
PubMed1982

PGM1a subtyping in Western Germany (Düsseldorf region).

Phosphoglucomutase (PGM1a) subtypes were determined by isoelectric focusing on samples of 496 unrelated individuals. Ten phenotypes were observed as gene products of four alleles at the PGM1 locus, with the following gene frequencies: PGM1a1 = 0.631, PGM1a2 = 0.194, PGM1a3 = 0.126, and PGM1a4 = 0.049. A rare phenotype PGM1(8-a2) was observed.

Chromosome Mapping↗
PubMed1980

Spinach chloroplast mRNA for a 32 000 dalton polypeptide: size and localization on the physical map of the chloroplast DNA.

The RNA from chloroplasts of young spinach plants contains mRNAs which are translated in a cell-free protein synthesizing system from lysed rabbit reticulocytes. Using [35S]methionine to label the products of translation, the main peptides have apparent molecular weights of 55,000, 45,000, 40,000, 32,000, 20,000 and 17,000. The sizes of the mRNAs as estimated by sucrose gradient centrifugation under non-denaturing conditions are between 12 and 23 S. A prominent product synthesized with RNA isolated from young spinach plants has an approximate molecular weight of 32,000. The mRNA for this 32,000 dalton protein sediments at 14 S on sucrose gradient. The position of the DNA sequence coding for this mRNA on the restriction site map of spinach chloroplast DNA was determined by hybridization and hybrid-arrested translation. A preliminary transcription map shows that in RNA from spinach chloroplasts which is larger than 10 S different RNA species are present which hybridize to nearly all fragments of the larger of the two single-copy regions of the chloroplast DNA.

Chloroplasts↗
PubMed1979

Fractionation and identification of spinach chloroplast transfer RNAs and mapping of their genes on the restriction map of chloroplast DNA.

Spinach chloroplast 4S RNAs has been separated by two-dimensional polyacrylamide gel electrophoresis into about 35 species. After extraction from the gel, 27 of these RNA species were identified by aminoacylation as tRNAs specific for 16 amino acids. Individual tRNAs were labeled in vitro with 125I and hybridized to DNA fragments obtained by digestion of spinach chloroplast DNA with KpnI, PstI, SalI and XmaI restriction endonucleases. A minimum of 21 genes corresponding to tRNAs for 14 different amino acids have been localized on the restriction endonuclease cleavage site map of the DNA molecule. Of these, 15 genes corresponding to tRNAs for 12 amino acids are located in the larger of the two single-copy regions which separate the two inverted copies of the repeat region. Each copy of this repeat region contains a set of genes for the ribosomal RNAs and a gene for tRNA2Ile in the "spacer" sequence between the 16S and 23S ribosomal RNAs. The genes for tRNA1Ile, tRNA2Leu and tRNA3Leu also map in the repeat region, but outside the ribosomal DNA unit. At present, two more chloroplast tRNAs (for Pro and Lys) have been identified, but not mapped, while 4 unidentified 4S RNAs have been mapped in the large single-copy region of the DNA molecule. Evidence is presented that isoaccepting tRNA species can be transcripts from different loci.

Amino Acyl-tRNA Synthetases↗