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Biomedical subjects

A J Fairburn

Publications and source records attributed to A J Fairburn.

6 recordsLinked to original sources

Normal haematological and biochemical values for the swamp buffalo (Bubalus bubalis) in Australia.

Blood samples were collected from 24 immature male, 55 immature female and 99 mature female water buffalo kept at an experimental farm in the Northern Territory. Haematological analysis was performed on blood collected in dipotassium--ethylene diamine tetra acetic acid while biochemical analysis was performed on serum and plasma (for glucose) samples. Haematological values of mature buffalo were similar to those recorded for swamp buffalo in Malaysia. Blood cell appearances were similar to those reported for adult Indian river buffalo though values recorded for red cell components were higher. Statistical analysis revealed no significant differences between immature male and female buffalo. Red cell components, eosinophils, total plasma and serum proteins, albumin, gamma globulins, inorganic phosphate and the enzyme gamma-glutamyl transferase were significantly higher for mature female buffalo when compared to immature females. Reasons for the differences were not fully determined but the effect of age and nutritional status in combination with a variable period of domestication were considered.

Age Factors↗

Effect of various diets on faecal analysis in normal dogs.

Faecal analysis for undigested/unabsorbed food particles and protease activity was performed on multiple samples obtained from four normal dogs each fed four different diets. All four diets, irrespective of the dog, produced a consistent faecal appearance and microscopic findings for undigested/unabsorbed food particles. Cooked and raw minced meat diets produced similar results: stools, which were small, green-brown, firm and glutinous, had little starch, occasional undigested short muscle fibres and moderate amounts of soap. Stools from the dogs on Pal were large, red-brown, solid to semi-solid, and moist; they contained little starch, occasional short undigested muscle fibres and little fat. The Loyal diet produced large, light yellow, dry, friable stools which had variable but usually low starch, no muscle fibres and no fat. Faecal protease activity for the different diets was analysed statistically. Differences in faecal protease activity due to different diets were evident but complicated by differences due to individual dogs.

Animal Feed↗

Faecal analysis for maldigestion in pancreatectomised dogs.

Faecal analysis for protease activity and the presence of undigested/unabsorbed food particles was done for four consecutive days on four pancreatectomised dogs fed a commercial complete pet food (Pal). All dogs produced faeces of similar appearance. The stools were bulky, light red-brown, well formed but soft and had a slight rancid odour. Generally, microscopic findings for undigested/unabsorbed food particles were similar. Starch was usually present in large quantities and often visible macroscopically when stained with Lugol's iodine. Undigested muscle fibres ranged from none to four per low powered field. The presence of large numbers of neutral fat droplets was inconsistent in Sudan III A stained faecal smears but Sudan III B stained faecal smears usually contained at least 20 neutral fat droplets with diameters greater than 20 microns per high powered field. Faecal protease activity was consistently low and varied between 0.5 and 2.8 azoalbumin units with the azoprotein method. No protease activity could be detected using the X-ray film gelatin digestion test.

Animals↗

Postprandial changes in plasma urea and creatinine concentrations in dogs.

Changes in plasma urea and creatinine concentrations were compared in 6 dogs fed 3 meals (cooked meat, raw meat, and a soft-moist preparation) in a crossover fashion. Each meal produced changes in urea and creatinine values. Although increased urea values were seen after all meals, the effects on creatinine were varied; concentrations increased after feeding cooked meat, but decreased after consumption of raw meat or soft-moist food. Although the creatinine changes were less pronounced, the variable effect of diet complicates the interpretation of plasma creatinine concentration in evaluating renal function.

Animal Feed↗

Cervical myelography in dogs using iohexol.

Iohexol, a non-ionic water-soluble radiographic contrast medium, was used for cervical myelography in dogs (10-17 kg). The animals remained clinically normal following myelography, with no evidence of seizures. At 10 days after myelography there was a slight, but significant (p less than 0.05) increase in neutrophils in the cerebrospinal fluid; these cell numbers had returned to normal by the time of necropsy at 60 days after myelography. This investigation suggests that iohexol may be suitable for clinical myelography in the dog.

Animals↗