Biomedical subjects

A J Simmers

Publications and source records attributed to A J Simmers.

At least 19 recordsLinked to original sources

PubMed2001

The influence of tinted lenses upon ocular accommodation.

We determined the effect upon accommodative responses of tinted lenses prescribed for the relief of visual discomfort in a group of five long term lens wearers. Static and dynamic responses were measured under four viewing conditions (1) prescribed tinted lens (2) neutral density filter (3) tinted lens of complementary colour and (4) no absorptive lens. While similarity and normality of the mean stimulus-response functions between the four viewing conditions were evident, the low frequency component of the accommodation microfluctuations was significantly greater while viewing the target in the 'no lens' viewing condition. These increases in the low frequency components (LFC) of the accommodation may be a subtle indicator of visual stress in these patients. Colour specificity is not supported by this finding.

Accommodation, Ocular↗

PubMed2001

Snakes and ladders: the role of temporal modulation in visual contour integration.

We investigated temporal aspects of the cortical mechanisms supporting visual contour integration by measuring observers' efficiency at detecting fragmented contours, composed of Gabor micropatterns, embedded in a field of distractor elements. Gabors consisted of a static Gaussian enveloping a sinusoidal carrier which was temporally modulated by motion or counter-phase flicker. The elements forming the path could be oriented either parallel ('snakes') or perpendicular to the contour orientation ('ladders'). Sensitivity to contour structure (estimated by measuring the maximum tolerable element orientation jitter supporting contour detection) was increased when the elements were drifting or flickering. Snakes were more detectable than ladders under all conditions. The increase in sensitivity conferred by drifting carriers was present even when the elements in the same stimulus were drifting at a range of speeds spanning almost three octaves. These results lend further support to the notion that the contour integration system receives separate transient and sustained input.

Discrimination, Psychological↗

PubMed2001

Spatiotemporal visual function in tinted lens wearers.

PURPOSE: Tinted lenses have been widely publicized as a successful new treatment for reading disorders and visual stress in children. The present study was designed to investigate a variety of visual deficits reported by children who experience high levels of visual stress and perceptual distortions when reading (Meares-Irlen syndrome; MIS) and to assess the improvements in visual comfort they report when tinted lenses are worn. METHODS: Twenty children (13.1 +/- 0.9 years of age) were recruited who had successfully worn tinted lenses for at least 6 months and were compared with an age-matched control group (12.6 +/- 2.2 years of age) of 21 children who were not lens wearers. A range of psychophysical tasks was adapted to identify specific anomalous visual perceptions. Spatiotemporal contrast sensitivity and contrast increment thresholds were used to investigate subjective reports of dazzle and hypercontrast, and a minimum motion perception (D(min)) and a motion-coherence task were used to assess subjective reports of visual instability and motion. RESULTS: In all viewing conditions (with versus without lens), no selective functional visual loss was demonstrated with any of the tasks used. Psychometric functions also revealed no significant difference between subject groups (control versus MIS). CONCLUSIONS: Under thorough psychophysical investigation, these results revealed no significant difference in visual function between subject group, and this finding is consistent with the absence of any effect of the tinted lenses in the group with MIS.

Adolescent↗

PubMed2001

Deficit of visual contour integration in dyslexia.

PURPOSE: The visual processing of text occurs spontaneously in most readers. Dyslexic persons, however, often report both somatic symptoms and perceptual distortions when trying to read. It is possible that the perceptual distortions experienced by those with dyslexia reflect a disturbance in the basic mechanisms supporting perceptual organization at the early stages of visual processing. Integration of information over extended areas of visual space can be measured psychophysically in a task that requires the detection of a path defined by aligned, spatially narrow-band elements on a dense field of otherwise similar elements that are randomly oriented and positioned. In the present study a contour integration task was used to investigate such perceptual organization in dyslexia. METHODS: The detection of contours or paths composed of Gabor micropatterns was performed within a field of randomly oriented distracter elements in a 2-alternate forced choice (AFC) task. The stimuli were manipulated by randomly varying both the density of the background noise elements and the number of elements that defined a path of constant length. RESULTS: In all observers, sensitivity to the paths increased with the number of target elements comprising the path, and subjects in both groups exhibited similar trends in relative density of the stimuli. However, in all conditions, dyslexic observers were two to three times less sensitive to path stimuli than the control group. CONCLUSIONS: In the present study the authors have described a visual deficit in a global integration task in dyslexia. The pattern of deficits reported suggest that abnormal cooperative associations may be present in dyslexia that are indicative of poor perceptual integration.

Adult↗

PubMed2000

Visual function thresholds in children.

PURPOSE: The aim of the present study was to establish a range of values for individual visual function measurements in Primary 1 schoolchildren. METHODS: 93 visually normal children with a mean age of 5.4 +/- 0.3 years were recruited with parental consent. Measures of visual acuity obtained included 1) High Contrast (CAT) 2) Low Contrast (LC) 3) Single Letter (SLA) and 4) Repeat Letter Acuity (RRL). These measurements were randomised and obtained during the same visit. Hyperacuity thresholds were obtained at a separate visit, using both Vernier and oscillatory motion displacement stimuli (OMDT). Re-test data for all tests was collected from a random sample of 30 children after a period of at least two weeks. RESULTS: For all measures the distribution of scores was found to be normal. All visual acuity scores are in log units. Mean ( +/- SD) values for each test were as follows; CAT = 0.11 +/- 0.07; LC = 0.38 +/- 0.08; RRL = 0.04 +/- 0.07; SLA = -0.02 +/- 0.09; Vernier threshold = 69 +/- 21 arcsec; OMDT = 60 +/- 9 arc sec. The confidence limits of the test-retest discrepancies were as follows; CAT = +/- 0.05; LC = +/- 0.07; RRL = +/- 0.04; SLA = +/- 0.04; Vernier = +/- 17 arcsec; OMDT = +/- 6 arcsec. The confidence limits of the interocular discrepancies were as follows; CAT = +/- 0.08; LC = +/- 0.08; RRL = +/- 0.08; SLA = +/- 0.11; Vernier = +/- 14 arcsec; OMDT = +/- 11 arcsec. CONCLUSIONS: To fully describe an individual's visual capacity it is important that new tests of visual function are developed based on emerging knowledge of visual physiology. In this study confidence limits can be constructed for each visual function measure, test-retest and interocular discrepancies using the normal distributions found above. This will provide the clinician with further age-matched values and contribute to our understanding of functional visual development.

Child, Preschool↗

PubMed1999

The effect of abnormal fixational eye movements upon visual acuity in congenital nystagmus.

PURPOSE: The purpose of this study was to investigate the role that abnormal eye movements play in the degradation of visual acuity. METHODS: Visual acuity was measured monocularly in 10 normal subjects (26.7+/-4.3 years) and 5 subjects with congenital nystagmus (34.9+/-8.8 years), using Regan Repeat Letter charts (RRL) and a logMAR based test (LogMAR Crowded Acuity Test (CAT)) while eye movements were continuously recorded using a commercially available infrared limbal eye tracker (Type 54, Optoelectronic Developments, UK). The eye tracker was controlled via a virtual oscilloscope (Viewdac, Keighly Instruments, UK) on an IBM PC clone (Opus Technology 486). RESULTS: The mean visual acuity obtained with RRL was significantly higher than that obtained by CAT in the subjects with congenital nystagmus. A significant correlation was found between the root mean square value of the nystagmus waveform and the angular extent of CAT. Linear regression analysis revealed a correlation between the duration of the foveation periods and the linear acuity of the subjects with congential nystagmus. The nystagmus waveforms also demonstrated increased amounts of high frequency components (HFC: >3.0 Hz) when compared to the normal eyes. CONCLUSIONS: The results of this study reveal 1) RRL charts provide a measure of a subjects resolution limit which is less dependent on eye movements; 2) the duration of foveation periods has a significant effect on visual acuity measurements obtained using a linear test chart format; 3) the predominance of high frequency components in the congenital nystagmus waveforms lead to short foveation periods adding to the degree of fixation instability.

Adult↗

PubMed1999

Improvement of visual function in an adult amblyope.

PURPOSE: In this case report, the efficacy of occlusion therapy was investigated in a strabismic amblyope above the currently accepted age for treatment. Success was assessed not simply by a change in visual acuity, but by examining a number of parameters which relate to both sensory and motor aspects of visual function. METHODS: As well as routine orthoptic and optometric evaluation, additional tests were administered as follows: high and low contrast LogMAR Crowded Acuity, repeat letter acuity, and hyperacuity measurements. RESULTS: A functional loss in each of the tests used was demonstrated, and occlusion therapy appeared to improve all aspects of the amblyopia, with a significant difference in pre- and post-therapy results. CONCLUSIONS: The results of this study suggest that: (1) occlusion therapy can produce substantial improvements in visual function in adult amblyopia; (2) many aspects of visual function can improve beyond the traditional critical periods for development in amblyopia; and (3) with good patient compliance and cooperation, age should not be the critical factor in the initiation of treatment for amblyopia.

Adult↗

PubMed1999

Functional visual loss in amblyopia and the effect of occlusion therapy.

PURPOSE: The aim of this study was to define the nature of functional visual loss in amblyopia and to identify those subjects whose amblyopia is chiefly due to one or more of the following deficits: abnormal contour interaction, abnormal eye movements, abnormal contrast perception, or positional uncertainty. METHODS: Fifty amblyopic children with a mean age of 5.6+/-1.3 years were referred from diverse sources. In addition to routine orthoptic and optometric evaluation the principal visual deficits in the amblyopic eye of each subject were identified using the following measures of visual acuity: high contrast linear, single optotype, repeat letter and low contrast linear, plus Vernier and displacement thresholds. These measures were repeated as the children underwent a prescribed occlusion therapy regime, after parental consent. RESULTS: All amblyopic subjects demonstrated a functional loss in each of the tests used, and occlusion therapy appeared to improve all aspects of the amblyopia. High contrast visual acuity was not always the primary deficit in visual function, and when amblyopic subjects were divided according to their primary visual loss, this visual function was found to show the greatest improvement with treatment. CONCLUSIONS: These results suggest that to successfully identify the primary visual deficit and monitor the success of occlusion therapy it is necessary to assess other aspects of visual function in amblyopia.

Amblyopia↗

PubMed1999

Contour interaction for high and low contrast optotypes in normal and amblyopic observers.

We investigate the influence of stimulus contrast upon contour interaction in normal and amblyopic subjects. Using a computer generated acuity task, flanked and unflanked acuities were measured psychometrically at both high contrast (80%) and low contrast (6%), in a group of 19 normal and 11 amblyopic subjects. The crowding ratio for high contrast letters was found to be significantly higher than that for low contrast letters. The extent of the crowding zone was measured at high and low contrast by varying the separation of the optotype and flanking bars. The crowding zone measurement was repeated for the high contrast optotypes using dioptric blur. The position of the flanking contours was found to have a significant effect on letter resolution at high contrast but no significant effect was demonstrable at low contrast. With the addition of dioptric blur the effect of contour interaction became negligible at high contrast. These findings support the hypothesis that the crowding effect is: (1) similar in normal and amblyopic eyes when tested at threshold; (2) is contrast dependent appearing only for high contrast optotypes.

Adult↗

PubMed1997

Grating detection and orientation discrimination in amblyopia.

PURPOSE: We examined whether the misperceptions associated with amblyopic visual perception can be revealed under natural viewing conditions by comparing the ability to detect the presence of a grating with the ability to identify the grating orientation. METHODS: Grating detection and orientation discrimination performance (horizontal versus vertical) were determined, using stimuli that consisted of sinusoidal gratings of fixed contrast (75%) but with variable spatial frequency. A total of four amblyopic subjects (two strabismic and two non-strabismic) and four age-matched normals participated in the experiment. RESULTS: Psychometric functions for grating detection and orientation identification were found to be closely matched in the normal subjects and in all four amblyopic subjects, indicating that orientation could be correctly identified at detection threshold. CONCLUSIONS: The absence of orientation uncertainty in the psychophysical data for the amblyopic observers is not consistent with the several previous reports of spatial aliasing in the central field of amblyopes. Our results suggest that non veridical visual perception in central amblyopic vision can not be revealed under natural viewing conditions by comparing the ability to detect the presence of a grating with the ability to identify its orientation. Possible reasons for the failure of this technique to reveal spatial aliasing in amblyopes are discussed.

Adult↗

PubMed1997

Screening for amblyopia: a comparison of paediatric letter tests.

AIMS/BACKGROUND: The measurement of visual acuity is the most widely accepted indicator of amblyopia and is thought by some to be the only effective screening test. The aim of this study was to investigate the effectiveness of the traditional single optotype Sheridan-Gardiner test (SGT) in the measurement of visual acuity and the detection of amblyopia, compared with the log based linear format Glasgow acuity cards (GAC). METHODS: In the present study visual acuity was measured monocularly in 702 primary 1 schoolchildren using both acuity tests. RESULTS: A significant difference was found in the mean (SD) visual acuity measured with GAC (0.9 (0.08) modified logMAR) and SGT (1.13 (0.09) modified logMAR), df = 632, t = -59.08, p = 0.0001. The majority of children (89.3%) achieved visual acuities better than 6/6 in either eye when using the single optotype test. If the 95% confidence limits for a significant interocular difference in acuity are used as-criteria for the detection of unilateral amblyopia, GAC were found to be the most sensitive, correctly identifying 100%, while SGT identified 55% of the children with unilateral amblyopia. CONCLUSION: The results of this study highlight several problems with both the test format and testing procedure in the present school screening system.

Amblyopia↗

PubMed1994

5HT induces NMDA receptor-mediated intrinsic oscillations in embryonic amphibian spinal neurons.

The existence and possible contribution of intrinsic membrane potential oscillations to the generation of locomotor rhythmicity was investigated in spinal cord neurons of newly hatched Rana temporaria tadpoles, by intracellular recording from immobilized animals. The bath application of 100 microM N-methyl-D-aspartate (NMDA) evoked continuous swimming-like activity in ventral motor roots and rhythmic synaptic drive to ventrally located spinal neurons, presumed to be motoneurons. In 0.5 microM tetrodotoxin-treated preparations, similar applications of NMDA depolarized neurons by ca. 20 mV, but did not lead to intrinsic oscillatory activity, although some evidence for voltage-dependent membrane bi-stability was obtained. However, bath application of the neuromodulatory amine, serotonin (5HT; 5 microM), in the presence of NMDA and TTX, reversibly induced sustained membrane potential oscillations (up to 40 mV in amplitude) that were similar in waveform to those already described in other adult vertebrate motor systems. The TTX-resistant oscillations were dependent upon the presence of magnesium ions in the bathing solution and were abolished by the NMDA receptor antagonist, D-2-amino-5-phosphonovaleric acid (APV). The results suggest that in this simple, developing vertebrate locomotor system, the activation of 5HT receptors on spinal cord neurons in turn modulates NMDA receptor activation to enable the expression of intrinsic oscillatory membrane properties which could contribute to the generation of locomotor behaviour.

Action Potentials↗

PubMed1994

Oscillatory membrane properties of spinal cord neurons that are active during fictive swimming in Rana temporaria embryos.

We have sought evidence for intrinsic oscillatory membrane properties in spinal cord neurons which participate in fictive locomotion in Rana temporaria embryos. In the presence of tetrodotoxin to synaptically isolate intracellularly recorded neurons, the bath application of N-methyl-d-aspartate (NMDA) depolarizes neurons by 20 to 30 mV. It does not, however, elicit continuous membrane potential oscillations, as have been found in homologous spinal neurons of the lamprey. However, addition of the neuromodulatory amine 5-HT to the bathing medium rapidly induces repetitive large scale (up to 40mV) oscillations in membrane potential. These oscillations depend upon the presence of magnesium ions and are abolished by the NMDA antagonist, APV. They do not occur when 5-HT is applied in the absence of NMDA. Expression of oscillatory membrane behaviour thus appears to rely upon an interaction between 5-HT and NMDA receptors. Neuroanatomical evidence shows that endogenous 5-HT-containing descending projections are present in the ventrolateral margins of the spinal cord at this stage in development and could therefore trigger these oscillations during normal motor behaviour. Preliminary pharmacological studies support the conclusion that activation of 5-HT1a receptors is involved in the induction process.

Animals↗

PubMed1994

Electrical coupling and intrinsic neuronal oscillations in Rana temporaria spinal cord.

In the presence of TTX and NMDA, spinal cord neurons in Rana temporaria embryos generate membrane potential oscillations, but only when 5-HT is added to the perfusate. These oscillations are voltage-dependent due to magnesium block of the NMDA receptor ionophore and can vary in amplitude between 0 and 40 mV with imposed membrane polarization. In contrast, the intrinsic frequency of the oscillations is unaffected by changes in membrane potential. This could result from electrical coupling amongst homonymous motoneurons. Here we present initial evidence for such connections and discuss their implications for the segmental control of rhythmic motor behaviour.

Animals↗

PubMed1994

Presynaptic inhibition of primary afferent transmitter release by 5-hydroxytryptamine at a mechanosensory synapse in the vertebrate spinal cord.

The effects of the neuromodulatory monoamine 5-HT (serotonin) on a cutaneous mechanosensory (Rohon-Beard, R-B neuron) pathway in the spinal cord of postembryonic Xenopus laevis tadpoles have been examined. In paralyzed animals, exogenous 5-HT at 1-10 microM reversibly inhibits (within 1-2 min) the activation of fictive swimming in response to electrical stimulation of R-B free nerve endings in the skin. At threshold stimulus intensities for swimming under control conditions, intracellularly recorded EPSPs in contralateral motoneurons are completely abolished by 5-HT without any obvious change in neuronal conductance or membrane potential. However, increasing the stimulus voltage can activate swimming with enhanced motor burst discharge on each cycle (Sillar et al., 1992). This suggested that 5-HT inhibits the swim-initiating pathway rather than the motor rhythm-generating circuitry itself. Extracellular recordings from the central projections of R-B neurons indicated that the amine does not impair the generation of mechanoafferent impulses or their propagation into the spinal cord. However, 5-HT application blocks impulse activity in dorsolaterally positioned sensory interneurons (DLis) that are contacted by R-B neurons, suggesting that 5-HT acts at R-B to DLi synapses in the dorsal cord. By recording with microelectrodes from DLis, we find that skin stimulus-evoked EPSPs at this first-order synapse in the swim-initiating pathway are reversibly suppressed by 5-HT. No obvious change in DLi membrane potential or conductance could be detected during the inhibition, suggesting a presynaptic site of action for 5-HT. To investigate this suggestion further, the effects of 5-HT on the spontaneous release of R-B sensory transmitter (excitatory amino acid, EAA) were examined, again by recording postsynaptically from DLis. In quiescent preparations, DLis receive spontaneous glycinergic, GABAergic, and EAA receptor-mediated PSPs. The inhibitory potentials are abolished by strychnine and curare, respectively. The excitatory potentials that remain are not blocked by application of the calcium channel blocker cadmium chloride at 1 mM, but are suppressed by the EAA receptor antagonist kynurenic acid. They therefore resemble the TTX-resistant EPSPs described previously in Xenopus DLis (Sillar and Roberts, 1991), which are presumed to arise from the spontaneous liberation of EAA transmitter from R-B terminals. Bath application of 5-HT dramatically reduces the rate of occurrence of these spontaneous EPSPs consistent with a presynaptic locus for the inhibitory effects of 5-HT.(ABSTRACT TRUNCATED AT 400 WORDS)

Afferent Pathways↗

PubMed1992

Modulation of swimming rhythmicity by 5-hydroxytryptamine during post-embryonic development in Xenopus laevis.

During the first 24 h of post-embryonic development in Xenopus laevis, a rapid change in the neural activity underlying swimming occurs in which the duration of ventral root discharge on each cycle increases from a single compound impulse to discrete bursts of activity. Moreover, this change in motor output progresses rostrocaudally, suggesting that it could result from the influence of a descending neural pathway upon the spinal rhythm-generating circuitry during early post-embryonic development. To begin to examine whether serotonergic neurons of brainstem raphe nuclei might have a role in this swimming development, we have studied the effects of 5-hydroxytryptamine (5HT) on fictive swimming in embryonic and larval animals. As previously demonstrated for other vertebrate locomotor rhythms, we find that bath-applied 5HT enhances the duration of motor activity on each cycle of larval fictive swimming. In addition, our results show that the sensitivity of the swimming rhythm to exogenous 5HT follows a strict rostrocaudal gradient. In young embryos (stages 32-36) 5HT does not affect the duration of ventral root impulses per cycle; by the time of hatching (stage 37/38), rostral but not caudal discharge is enhanced, and by stage 42 (24 h post-hatching) 5HT can increase motor burst durations along most of the length of the animal. These reversible changes induced by bath-applied 5HT closely resemble the normal rostrocaudal development of burst discharge during swimming in animals some 12 h older.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1992

The post-embryonic development of cell properties and synaptic drive underlying locomotor rhythm generation in Xenopus larvae.

In the first 24 h of post-embryonic development, the motor rhythm underlying swimming in Xenopus laevis tadpoles changes from brief (ca. 7 ms) ventral root discharge in each cycle to bursts of activity lasting around 20 ms (Sillar et al. 1991). Because individual motoneurons in the spinal cord of newly hatched embryos normally fire only a single impulse per cycle, two possible changes underly the transition to motor bursts seen in larval ventral roots; desynchronization of neurons in a given ventral root which continue to fire once per cycle, or the developmental acquisition of a multiple spike capability in individual motoneurons. Here we have recorded intracellularly from ventrally positioned spinal neurons, presumed to be myotomal motoneurons, in stage 37/38 embryos and 24 h later in development in stage 42 larvae. We find that (i) larval neurons are able to fire more than one impulse per cycle of fictive swimming activity; (ii) unlike in the embryo, they generally will fire multiple impulses in response to injected depolarizing current; (iii) the synaptic drive to motoneurons during swimming increases dramatically in complexity, although it still consists of alternating phases of synaptic excitation and chloride-dependent inhibition, superimposed upon tonic synaptic depolarization. The results therefore suggest a developmental change in the membrane properties of rhythmically active neurons as a major factor in the post-embryonic development of swimming in Xenopus larvae. This change appears to occur in premotor rhythm generating interneurons as well as in the motoneurons themselves and may satisfy a demand for behavioural flexibility that allows larvae to survive in a complex and changing environment.

Animals↗

PubMed1991

The development of swimming rhythmicity in post-embryonic Xenopus laevis.

The post-embryonic development of 'fictive' swimming in immobilized Xenopus laevis tadpoles has been examined during the first day of larval life. In Xenopus embryos (stage 37-38; Nieuwkoop & Faber 1956), the rhythmic ventral root activity underlying swimming occurs as single brief (ca. 7 ms) compound impulses on each cycle. However, by stage 42 (about 24 h after hatching), ventral root discharge consists of bursts lasting around 20 ms per cycle. In addition to increased burst duration in each cycle of larval swimming, the range of cycle periods within an episode increases, although mean period values (ca. 70-80 ms) remain similar to those of the younger animal. Consequently, motoneurons at developmental stage 42 are active during swimming for a greater percentage (ca. 25%) of cycle time than at stage 37-38 (ca. 10%). Developmental stage 40 (ca. 12 h post-hatching) is an intermediate stage in rhythm development. Ventral root discharge varies from bursts of 10-20 ms at the start of an episode to embryonic (ca. 7 ms) spikes at the end of an episode. Furthermore, discharge varies from bursts of activity in rostral segments of stage 40 larvae to 7 ms spikes more caudally, as in embryos. The data thus suggest that Xenopus swimming rhythmicity develops relatively rapidly, along a rostrocaudal gradient, and may involve acquisition of multiple spiking in spinal neurons.

Animals↗