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Biomedical subjects

A Jan

Publications and source records attributed to A Jan.

At least 19 recordsLinked to original sources
PubMed2004

Microarray analysis of brassinosteroids- and gibberellin-regulated gene expression in rice seedlings.

In order to understand the molecular mechanisms by which two types of phytohormones, brassinosteroids (BRs) and gibberellins (GAs), regulate plant growth and development, a cDNA microarray was made containing 4000 clones randomly selected from a rice cDNA library constructed from RNA isolated from seedlings treated with GA(3) and brassinolide (BL). The array was then probed to identify differences in expression using labelled cDNAs prepared from treated and control seedlings. Nine and 29 unique cDNA clones were up-regulated, while 32 and 42 unique cDNA clones were down-regulated by BL and GA(3), respectively. The predicted products of these BL- and GA-regulated genes fall into such functional categories as signal transduction, transcription, metabolism, cellular organization, and defense or anti-stress responses. Northern analysis of the selected BL- and GA(3)-regulated genes not only confirmed their BL or GA(3) dose dependent expression, but also revealed significant differences in the degree of expression in different organs and in response to other phytohormones. These results demonstrate that BR and GA influence growth and development by coordinately regulating the expression of specific groups of genes.

Brassinosteroids↗
PubMed1997

Evaluation of dowdo (wheat-milk gruel) in children with acute diarrhoea.

Appropriate feeding practices have an important impact on diarrhoeal disease management in developing countries. We evaluated the efficacy of feeding dowdo, a wheat-milk gruel, traditionally used as a weaning food in the Northern Areas of Pakistan. Dowdo was compared with khitchri, a rice-lentil mixture, in acute diarrhoea through a randomized trial. Seventy- six children between 6 and 36 months of age, with acute watery diarrhoea of less than seven days were recruited. After rehydration with standard World Health Organization (WHO) glucose-based oral rehydration solution or intravenous Ringers lactate, patients were randomly assigned to either diet group. Dowdo and Khitchri were found to be equally effective in terms of stool frequency and output, duration of diarrhoea, weight gain and duration of hospitalization. The results indicate that feeding dowdo was as effective as khitchri in children with acute diarrhoea. Additionally, acceptability of dowdo was better than Khitchri. It is recommended that dowdo be used for nutritional management of diarrhoeal disease in children in the Northern Areas of Pakistan.

Acute Disease↗
PubMed1990

[Isolation of human immunodeficiency virus from Hungarian AIDS and ARC patients].

Human immunodeficiency virus infected persons are usually identified by indirect methods, detecting viral-specific antibodies. Helping the early diagnosis of HIV infection there is a need to detect virus or viral specific antigens directly. Virus isolation have been attempted from separated lymphocytes of HIV infected five homosexual male patients and one transfusion recipient. Three patients had AIDS, three belonged to the ARC group at the time of the examination. In 3 out of 6 lymphocyte cultures cocultivated with normal donor lymphocytes, virus antigens and virus replications had been detected within ten days of culture. The amount of HIV antigen p24 ranged between 0.5----2.0 ng/ml during the first two weeks of cocultivation. Permanent human lymphoid and monocyte/macrophage cell lines have been infected by the viruses isolated from the primer lymphocyte cocultures. Productive infection could be initiated in Jurkat tat-III and U937 cells, while infection of Jurkat and HUT 78 cell lines was transient. Results indicate, that HIVs could be isolated from infected Hungarian patients with various stages of AIDS and one isolate--termed HIV-1FB918--actively replicates in human permanent cell lines.

AIDS-Related Complex↗
PubMed1990

A technique for one stage repair of complete palatal cleft.

A technique for repairing in one stage, wide complete palatal cleft of congenital origin with obvious shortening of the soft palate, is described. Mucoperiosteal flaps were obtained from vomer bone and palate while muco-muscular flap from posterior pharyngeal wall by means of a simple technique. A layer closure was used to fill in the palatal defect. This technique was used successfully in 24 out of 25 cases, with a mean follow-up of 2 years. When fully healed, the palate attained a normal shape and function.

Child↗
PubMed1990

A new look at the nasal adhesions.

Formation of nasal adhesions is a troublesome complication following nasal surgery. They are more commonly seen after combined surgical procedures on the nose, but may also appear after a single procedure. We present and discuss this problem following correction of the nasal septum through submucosal resection (S.M.R.).

Adolescent↗