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A Jaques

Publications and source records attributed to A Jaques.

5 recordsLinked to original sources
PubMed1993

Use of hydrazine to release in intact and unreduced form both N- and O-linked oligosaccharides from glycoproteins.

The use of hydrazine to release unreduced N- and O-linked oligosaccharides from glycoproteins has been investigated using several "standard" glycoproteins of previously defined glycosylation. It is shown that hydrazinolysis can be used to release intact N- and O-linked oligosaccharides in an unreduced form. The release of O-linked oligosaccharides occurs with a lower temperature dependence than the release of N-linked oligosaccharides, and the kinetic parameters governing release of oligosaccharides from these standard glycoproteins have been determined. These parameters allow a definition of reaction conditions under which anhydrous hydrazinolysis can be used to selectively release O-linked oligosaccharides (60 degrees C, 5 h) or release both N- and O-linked oligosaccharides (95 degrees C, 4 h) in high yield (> 85%) from all glycoproteins investigated (n = 11). Under these reaction conditions, the recovered N- and O-linked oligosaccharides are structurally intact (as judged by 600-MHz 1H-NMR, laser-desorption mass spectrometry, HPAEC-PAD, gel filtration, and glycosidase digestion), with the possible exception of certain N- and O-acyl substituents of sialic acid. This use of mild hydrazinolysis therefore allows both the simultaneous and sequential chemical release from glycoproteins of O- and N-linked oligosaccharides in their intact unreduced form.

Animals↗
PubMed1985

Morphogenesis of the elastic fiber: an immunoelectronmicroscopy investigation.

In this study a rabbit antiserum against human aortic elastin, which showed a high degree of species specificity in ELISA tests, was used to examine elastin fiber formation in the human fetal aorta between the ages of 14 and 23 weeks. Elastin was first detected by the antibody in the matrix of the 14-week-old specimen in association with the microfibrillar component. At this stage of development, the sections did not reveal structures morphologically identifiable as elastin. By the 17th week, discrete loci of elastin deposition were observed together with well-defined elastin fibrils. Only by the 23rd week did the aorta show the characteristic layering of elastic fibrils separating the myoblasts of the tunica media. In the latter specimen, the newly synthesized uncrosslinked elastin appeared to be unevenly distributed on the surface of elastin fibrils where it formed continuous strips of variable width arranged mostly in the form of spirals. This observation is discussed with respect to the proposals that the morphogenesis of elastic tissue is a dynamic process involving a close interrelationship between elastic fibrils and elastogenic cells and the morphogenetic movement of elastogenic cells plays an important role not only in the growth of elastic fibrils but also in the ultrastructural organization of the tissue.

Actin Cytoskeleton↗
PubMedDate not supplied

Immunolocalization of a 35K structural glycoprotein to elastin-associated microfibrils.

In this study, a rabbit antiserum directed against a 35K glycoprotein (35K-GP), extracted from connective tissue, was used to examine the localisation of this antigen within foetal bovine ligamentum nuchae at stages of development preceding (4th month) and coinciding with (7th month) active elastin biosynthesis. In these tissues the antibody, detected by a colloidal gold conjugate technique, localised specifically to 11-nm fibrils, identified as the microfibrillar component, present both in the form of independent bundles and in association with elastin in the developing elastic fibres. No other connective tissue component was recognised by the antibodies which had been purified by affinity chromatography. The ability of the antibodies to bind to the microfibrils appeared to be dependent on embedding in LR White resin, as colloidal gold binding was greatly reduced in tissue embedded in epoxy resin. These results are discussed with respect to the role that 35K-GP may play in the morphogenesis of the elastic fibre.

Animals↗