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Biomedical subjects

A K Baltrashevich

Publications and source records attributed to A K Baltrashevich.

At least 19 recordsLinked to original sources
PubMed1989

[A study of the features of E. coli bacteroid infection in experiments on mice].

Experiments on the intraperitoneal infection of F1 (CBA X C57BL/6) mice with bacteroids of the group Fragilis in combination with E. coli have revealed the presence of a synergic effect leading to more pronounced (greater frequency of abscess formation) and even qualitatively new (necrotic lesions of the liver) pathological changes in comparison with those observed in monoinfections. Bacteroides fragilis strain 1/1087 gamma has been shown to have greater virulence than that of Bacteroides vulgaris strain 18; their virulence was determined from the capacity of microorganisms for survival in the abdominal cavity of mice, as well as from their capacity for inducing the formation of necrotic lesions and abscesses.

Animals↗
PubMed1985

[Rapid methods in culturing nonsporulating anaerobes and determining their antibiotic sensitivity by using disks].

A review of methods for determination of sensitivity of nonsporulating anaerobic bacteria to antibacterial drugs with paper disks is presented and the prospects for using the method of cultivation in solid media in Petri dishes under a polymer film are discussed. It is shown that cultivation at a temperature of 42 degrees C for obtaining earlier results instead of the temperature of 37 degrees C usually used for cultivation has no advantages. With an account of the result variability depending on the inoculation dose and other factors it is necessary to determine the boundary values of the growth inhibition zones for resistant, moderate sensitive and sensitive strains with respect to every antibiotic.

Anti-Bacterial Agents↗
PubMed1984

[Determination of the ecological niche of Bacteroides in the gastrointestinal tract--a study of a model of sterile intestines in germ-free animals].

The interrelations of the population of microorganisms belonging to the genus Bacteroides with the structures of the mucous membrane of the gastrointestinal tract of germ-free guinea pigs and mini-pigs have been studied. A considerable part of the population of these anaerobic microorganisms is associated in some way with the intestinal mucosa; at least, quite a number of these organisms inhabit mucins covering the mucosa. The determination of this ecological niche occupied by bacteroids in the intestinal microbiocenosis suggests that these organisms have considerable influence on the physiological functions and pathological states of the digestive tract.

Acetobacter↗
PubMed1983

[Determination of the enzymatic traits of anaerobic gram-negative nonsporulating bacteria].

The method requiring the use of a semi-liquid medium with the reduced content of protein hydrolysates and with bromo-thymol blue added as indicator and permitting the determination of sugar fermentation by bacteroids and fusobacteria is presented. The determination is made by a change in the color of the indicator and the intensity of microbial growth (opacity) after the incubation of the inoculated medium under free access of air. A study has been made for comparing the above method with the electrometric method for the determination of fermentative features in anaerobic microorganisms.

Anaerobiosis↗
PubMed1982

[Method of determining the antibiotic sensitivity of anaerobic microorganisms using standard disks].

Three variants of the procedure for determination of antibiotic sensitivity in anaerobic microorganisms with the use of standard paper discs were developed. According to the first variant the solid nutrient medium is melted at 46 degrees C and mixed with the culture of the microbe being tested. The mixture is added to the cover of a Petri dish. When the medium becomes solid, antibiotic sensitivity discs are placed onto the agar surface. After that one more layer of the medium is added. The medium is allowed to solidify and some more medium is poured near the cover edge. Immediately after that the Petri dish is placed with its flat surface onto the agar layer in its cover. According to the first and second variants the mixture of the medium and culture is added to a Petri dish and immediately a transparent gas-proof polymer film of the dish size is placed onto the agar surface. Previously antibiotic paper discs or solutions are fixed on the films. THe incubation temperature for all three variants is 37 degrees C. The procedure allows one to observe the culture growth and to obtain the results earlier than in case the culture is incubated in an aerostate. The procedure is simple and saves labor and time.

Anaerobiosis↗
PubMed1982

[Comparative study of different substrates as the nutrient base of a culture medium for Bacteroides and Fusobacterium].

The results of the comparative study of different substrates prepared from foodstuffs and inedible raw materials and intended for use as the nutrient base of a medium for cultivating the organisms of the genera Bacteroides and Fusobacterium have demonstrated that commonly used nutrient bases prepared from meat, fish, casein and inedible animal blood (blood substitutes) are suitable for this purpose. The dried nutrient base prepared from the mixture of outdated blood substitutes (hydrolysin and casein hydrolysate) (see text for Russian letters) has been found to ensure the optimum growth of Bacteroides and Fusobacterium.

Animals↗
PubMed1982

[Frequency of detection and the species composition of anaerobic gram-negative nonsporulating bacteria in suppurative pathology in emergency surgery].

The improved technique of cultivation allowed to detect obligatorily anaerobic microorganisms in 58 out of 94 samples from patients with purulent inflammatory processes. The results of the studies showed that anaerobic microorganisms were most frequently isolated from the exudate of the abdominal cavity and the purulent discharge of wounds in peritonitis patients. Gram-negative nonsporulating bacilli made up 25.3% of all the isolated cultures. The identification of these microorganisms allowed to determine that in the exudate of the abdominal cavity, the discharge of surgical and posttraumatic wounds, and of the pleural cavity the representatives of the family Bacteroidaceae mainly occurred. Most frequently B. thetaiotaomicron and B. fragilis were isolated.

Abdomen↗
PubMed1978

[Obtaining and rearing germ-free miniature piglets for biomedical research].

The method of obtaining and rearing germfree miniature piglets for medical-biological research is described. The gnotobiotes were reared in rigid organic glass isolation cells up to 3 months. Milk diets supplemented with minerals and vitamins were used for nutrition. The gnotobiotes gained weight well during the whole observation period.

Animal Husbandry↗