Biomedical subjects

A K Deisingh

Publications and source records attributed to A K Deisingh.

3 recordsLinked to original sources

PubMed2006

Aptamer-based biosensors: biomedical applications.

This chapter considers the use of aptamer-based biosensors (generally termed 'aptasensors') in various biomedical applications. A comparison of antibodies and aptamers is made with respect to their use in the development of biosensors. A brief introduction to biosensor design and theory is provided to illustrate the principles of the field. Various transduction approaches, viz. optical, fluorescence, acoustic wave and electrochemical, are discussed. Specific biomedical applications described include RNA folding, high-throughput screening of drugs, use as receptors for measuring biological concentrations, detection of platelet-derived growth factor, protein binding and detection of HIV-1 Tat protein.

Animals↗

PubMed2004

Strategies for the detection of Escherichia coli O157:H7 in foods.

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Antigens, Bacterial↗

PubMed2001

Sequences of E. coli O157:H7 detected by a PCR-acoustic wave sensor combination.

In this paper, we report on the amplification by polymerase chain reaction (PCR) of a 509 base sequence unique to E. coli O157:H7. Immobilization of a probe for the bacterium on an acoustic wave sensor by the biotin-neutravidin interaction was employed to detect the on-line hybridization of the sequence with the sample obtained from PCR. The limit of detection was found to be 10(-8) M, which suggests that the method may be useful for detecting the organism in food, water and clinical samples. Finally, this approach lays the groundwork for incorporating the method into an integrated system for rapid PCR-based DNA analysis.

Acoustics↗