Biomedical subjects
Publications and source records attributed to A K Lokapur.
After activation of carboxyl groups with carbodiimide, heparin binds to collagen by a stable covalent linkage. Covalent binding of heparin to collagen results in enhanced stability of the preparation. Also, heparin bound collagen retains its anticoagulative activity.
Carboxyl groups of heparin were activated by carbodiimide (EDC) to allow stable covalent binding of heparin to various arterial reconstructions. Ammonium hydroxide treated Human Umbilical Veins Grafts (HUVG) outperform carotid arteries of goat, porcine and canine origin in both heparin loading and stability. In-vitro studies reveal that heparin bound HUVG retains its anticoagulative activity, showing promise towards the development of a nonthrombogenic small-caliber vascular prosthesis.