Biomedical subjects

A Kot

Publications and source records attributed to A Kot.

15 recordsLinked to original sources

PubMed2001

[Effect of intensive prenatal and diabetic care on newborn of diabetic mothers--14 years of observation].

UNLABELLED: Modern management of pregnancies complicated by maternal diabetes mellitus has in view delivery at term an healthy child with correct body mass and in good metabolic condition. THE AIM: Of our work was estimation of influence of intensive prenatal and diabetical cares on frequency of occurrence of failures and complications at newborns of mothers with pregestational diabetes mellitus delivered in period from 1987 to 2000. Study include 228 of newborns of diabetic mothers of class B-RF according to White, born in our hospital between 25 and 40 weeks of pregnancy. In aim of estimation of percentage of perinatal mortality, congenital malformations, prematurity, hyper- and hypotrophy in examined population of new-born children of mothers with PGDM, material was subdivided into 3 groups in 4 and 5-years intervals: group I (n = 96) 1987-1990, group II (n = 72) 1991-1995, group III (n = 60) 1996-2000r. RESULTS: Systematical decrease of percentage of perinatal mortality in examined groups from 7.3% to 5.0% was ascertained. There was a significant association between decreasing of number of congenital malformations in investigated new-born population from 12.5% to 5.0% and 2,5-times increasing of percentage of women with PGDM managed by combined team of diabetologist and obstetricians before conception. We observed an essential decreasing of percentage of prematurity in investigated material from 51.6% to 20.0%. Percentage of births of new-born children with macrosomy on space analysed of years was comparable. (13.7%, 14.1% and 15.0%) in spite of increasing of percentage of childbirths at term from to 80%. We did not ascertain increasing of percentage of incidents of IUGR. The percentage of IUGR in investigated groups carried out 5.3%, 5.6% and 5.0% in spite 2-times increasing of percentage of births of children of mothers with diabetes in class R and RF according to the White classification. CONCLUSIONS: Well controlled diabetes, intensive management of diabetic women by combined team of diabetologists and obstetricians on space 14 of years of activity of our Department due to decreasing of percentage of: perinatal mortality from 7.3% to 5.0%, congenital malformations from 12.5% to 5.0% and premature deliveries from 51.6% to 20.0% in population of new-born children of mothers with PGDM.

Adult↗

PubMed2001

[Determination of content of zinc and cooper in table and therapeutic mineral water].

Mineral table water and therapeutic water are good sources of macro- and microelement. Increase of consumption and variety of kinds of mineral water was the reason for undertaking this research. Contents of zinc and copper were determined in 13 kinds of mineral table water and in 12 kinds of therapeutic water by Atomic Absorption Spectrometry method (AAS) in Pye-Unicam SP0192 apparatus, after earlier concentration. Content of copper in mineral table water was from 0.4 microgram/l in "Krynica Zdrój" water to 11 micrograms/l in "Mazowszanka" water and in therapeutic water and from 1.7 micrograms/l to 22.6 micrograms/l. Content of zinc in mineral table water was from 7 micrograms/l to 640 micrograms/l and in therapeutic water was from 24 micrograms/l to 88 micrograms/l.

Copper↗

PubMed1998

Substrate-dependent cadmium toxicity affecting energy-linked K+/86Rb transport in Staphylococcus aureus.

Bacteria accumulate high amounts of potassium in the cytoplasm. For studying transport of K+ (with 86Rb as a marker) in bacteria (Staphylococcus aureus 17810S), the cells were depleted of the internal K+ pool by a DNP treatment. Kinetics and energetics of 86Rb transport was assayed with glucose as an exogenous energy source. It was shown that 86Rb uptake proceeded via a low affinity K+ transport system with an apparent K(m) of 2.3 mmol/L Rb+. Studies with the lipophilic cation TPP+ (tetraphenylphosphonium), the protonophore CCCP (carbonyl cyanide 3-chlorophenylhydrazone) and inhibitors (HQNO--2-heptyl-4-hydroxyquinoline N-oxide; iodoacetate) indicated that 86Rb transport was driven by delta psi (membrane potential) generated via the respiratory chain. The effect of Cd2+ on 86Rb transport was assayed with two energy donors--glucose and L-lactate. It was found that Cd2+ strongly inhibited delta psi-dependent 86Rb transport energized by cadmium-sensitive glucose oxidation, but was not toxic when cadmium-insensitive L-lactate was used as an energy source. The mechanism of these differential, substrate-dependent effects of Cd2+ on 86Rb transport is discussed.

2,4-Dinitrophenol↗

PubMed1998

Addressing the needs of a clinical service in the operational constraints of commissioning.

Limited resources, increased demand and current commissioning structures has put the rationalisation of health care high on the agenda. Any prioritisation process needs to be clinically led and client driven so reactive ad hoc arrangements are minimised and structures for good practice enabled. This paper argues that the current context be used as an opportunity to critically evaluate the services speech and language therapy aim to provide. By defining our aims and demonstrating the range and boundaries of our effect we can be proactive in structuring our services appropriately. Such an exercise currently underway in City and Hackney is outlined which aims to: describe an optimum structure for provision and the resources required, propose possible models within current resource and highlight the gap between the two.

Child↗

PubMed1995

[Lead, cadmium, copper and zinc content in vegetables, gooseberry fruit and soil from gardening plots of Lublin].

Lead, cadmium, copper and zinc contents in vegetables, fruits of gooseberries and in soil of Lublin gardening plots "Pionier" and "Podzamcze" both situated along heavy traffic streets, and "Pionier" additional to the close vicinity of automobile factory were determined by atomic absorption spectrophofometry. Statistical analysis of the results respected the streets or factory distances from the gardening plots, and the species of the vegetables. Levels of the determined elements in the most of the samples were lower than permitted by the Ministry of Health Regulation established in 1993. According to that Regulation a slight exceeding of zinc content in the red beetroots and in the overground parts of the leak were found. Lead content in the parsley root and in the dill was close to the tolerance limit. Both lead and cadmium of the soil of "Podzamcze" gardening plot significantly exceeded the levels considered as tolerable for unpolluted grounds, whereas the soil of "Pionier" contained lead close to limit of tolerance level.

Cadmium↗

PubMed1994

Sub- and supercritical fluid chromatography on packed columns: a versatile tool for the enantioselective separation of basic and acidic drugs.

Various chiral stationary phases were investigated in sub- and supercritical fluid chromatography for the separation, without derivatization, of basic (beta-blockers, benzodiazepines) and acidic (nonsteroidal anti-inflammatory drugs, beta-agonists) pharmaca. For all racemates, baseline separation was achieved within short analysis times. For several solutes, the high resolution obtained allowed injection of milligram amounts and semipreparative collection of the enantiomers. The parameters affecting enantioselectivity (column efficiency, influence of modifiers and basic or acidic additives, and temperature) have been studied. Enantiomerization of 3-OH-benzodiazepines could be suppressed by working at low temperatures or by using acetonitrile as a comodifier. Serial coupling of different chiral stationary phase columns resulted in a column triplet (Chiralpak AD, an amylose derivative; Chiralcel OD, a cellulose derivative; and Chirex 3022, a Brush-type with pi-donor characteristics) on which all solutes investigated could be baseline separated.

Adrenergic beta-Agonists↗

PubMed1991

[Evaluation of heavy metal contamination of potatoes from various regions of Poland].

Mercury, cadmium and lead contents were determined in 210 samples of potatoes of five cultivars, originating from four regions of Poland (Tarnobrzeg Province, Southern Region, Middle-Eastern Region, Poznań Province). Analysis was performed by the ASA method. Cadmium and lead were determined by the extraction technique, after metal complexing with APDC. Methyl isobutyl ketone was the organic phase. "Dry" mineralization of samples for cadmium and lead determinations was performed in a muffle furnace at 400 degrees C. Mercury was assayed by the cold vapour technique after "wet" mineralization with sulphuric and nitric acid in a Bethe apparatus. Mean mercury content was ca. 5.5 micrograms/kg for the samples from the uncontaminated regions; samples from the Southern Region contained on the average 12.9 micrograms/kg, i.e. twice more. Cadmium contents in potatoes from all the investigated regions were increased by an average of 0.029-0.064 mg/kg. Lead content in single samples was between 0.015-0.500 mg/kg; mean Pb contents in samples from the Southern Region, as compared with the Lublin region, were about twice higher. Hg, Cd and Pb contents were determined in the same samples of peeled potatoes and of their peels, this allowing for evaluation of the distribution of the investigated heavy metals in the tuber. It was found that lead occurs in the peels in 60-70%, and in the pulp in only 30-40%. As concerns cadmium and mercury, these metals appear in the tuber in 70-80%, and the peels in only 25-30%.

Cadmium↗

PubMed1987