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Biomedical subjects

A Kozlowska

Publications and source records attributed to A Kozlowska.

8 recordsLinked to original sources
PubMed1992

Defined medium supporting development of cleansed Plasmodium berghei ookinetes in Anopheles stephensi.

Hamsters blood infected with Plasmodium berghei was cultured in vitro for the development of ookinetes. The ookinetes were separated from blood components, suspended in various defined media and fed to Anopheles stephensi through a membrane. The development of the oocysts and infective sporozoites was recorded. Mosquitoes infected with ookinetes suspended in L15 formulated into L15-B, L15-D (a medium specially modified for this purpose), IPL-41 or 199 media with no proteins added, developed at least as many oocysts as the control mosquitoes fed ookinetes suspended in blood. Ookinetes suspended in the L15-B medium yielded more oocysts than after feeding ookinetes suspended in L15-B with 5% casein. Sporozoites from mosquitoes maintained on blood, L15-B, L15-D, or L15-B with 5% casein were shown to be infective to hamsters. Mosquitoes fed ookinetes suspended in sucrose solutions showed very few oocysts, but the yield was increased when a blood meal was given 2-4 days after the infective meal. Some of the oocysts which had developed from the ookinetes suspended in artificial media were found to have degenerated. The described system could be potentially useful for a study of the interaction between the vector physiology and the parasite. The possible use of the system to learn which media should be developed in the future for in vitro cultivation of oocysts is discussed.

Animals↗
PubMed1991

The effect of defined media, additive nutrients and metabolites on the development of the sporogony cycle of Plasmodium berghei in Anopheles stephensi.

Anopheles stephensi mosquitoes were infected with Plasmodium berghei by feeding on parasitaemic hamsters. After the infective blood meal they were separated into groups that were maintained on sugar solutions containing different additives. The numbers of oocysts developing in the various groups were then compared. When either casein, haemoglobin or foetal bovine serum was added to the sugar, the yield of oocysts was 1.6-2.1 times higher than that in controls fed only on sugar solutions. When either medium 199, Leibovitz's formulated L15 (L15-B) or 'B' compounds alone were added, the yield of oocysts was 2.3-2.9 times higher than that in the controls. The addition of p-aminobenzoic acid, hypoxanthine, methiolnine, or fractions of 'B' had no significant effect on the number of oocysts.

Animals↗
PubMed1978

Contact sensitivity to trivalent chromium compounds.

The investigations confirmed the known fact that for skin tests larger amounts of trivalent chromium compounds are necessary than those of potassium dichromate. It was also observed that some patients react to relatively higher Cr VI and lower Cr III concentrations and others vice versa. Chromium nitrate and chloride release and that of potassium dichromate was determined and converted to the chromium content in the concentrations used for skin tests. It was found that Cr III compounds cannot be used for skin tests in a 0,5% concentration if only because they are released in undeterminable amounts. Release of Cr III and potassium dichromate from bases containing the same amount of substance when converted to chromium demonstrated that the different release of Cr III is not an additional cause of the fact that much higher concentrations of Cr III compounds have to be used for patch tests than those of Cr VI compounds.

Chromates↗
PubMed1976

Sensitivity to some rubber additives.

The frequency of positive patch tests to 13 rubber accelerators in Poland was recorded. Of particular interest was the relatively large group of patients positive to several, mostly chemically unrelated, accelerators. The mechanism of this multiple sensitivity is discussed. Positive tests to N-isopropyl-N-phenyl-p-phenylenediamine were observed in more than half of the patients reacting to several aromatic amines. Anamnesis indicated that in the case of cross-reactions to many aromatic amines, none of the patients positive to N-isopropyl-N-phenyl-p-phenylenediamine had complained of a reaction to rubber products.

Age Factors↗
PubMed1975

[Clinical study of resorbable surgical sutures. A suggestion].

There has been attempted in the Institute of Surgery, Wrocław Medical School, in 1975 the clinica study with the purpose of comparative evaluation of two kinds of tissue-resorbable surgical sutures, namely catgut and polyglycolic acid threads. To standardize the basic methods of clinical examination to be followed by teams of experts, basing on literature data and own experience, a proposition was elaborated including indications and methods of choosing the groups of resorbable and non-resorbable threads, aseptic demands, and intraoperative, postoperative and laboratory examinations.

Biological Products↗
PubMed1975

[Laboratory and clinical testing of modern adhesive tapes and dressing materials].

Twelve kinds of adhesive tapes and dressing materials produced by Beiersdorf were evaluated using laboratory, experimental and clinical tests. There were performed examinations of physical, physico-mechanical and bacteriostatic properties. Tests for supradermal and intradermal reactivity were performed in experimental animals. Moreover, different clinical tests of these materials were made.

Animals↗