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Biomedical subjects

A L Kheĭnaru

Publications and source records attributed to A L Kheĭnaru.

7 recordsLinked to original sources
PubMed1991

[Localization of camphor degradative plasmids on the chromosome of Pseudomonas putida strains PaW].

Camphor degradative plasmids (CAM, pRK1) are preferentially situated on chromosomes of Pseudomonas putida strains PaW. After having been transferred into Cam+ strains, the TOL plasmid pWWO dissociates into the cryptic plasmid pWWO-8 and chromosome-borne transposon Tn4651. The opposite situation, i.e. reconstruction of the TOL plasmid pWWO from the cryptic plasmid pWWO-8 and chromosome-borne catabolic operons of the pWWO plasmid has been described. Cam- derivatives of the CAM plasmid were obtained in vivo which contain the TOL plasmid transposons Tn4651 or Tn4652 as obligatory structural elements. These plasmids as well as pWWO-8 determine conjugational mobilization of chromosome-located cam operons followed by their integration into the chromosome of recipient.

Camphor↗
PubMed1990

[New plasmids of herbicide 2,4-dichlorophenoxyacetic acid biodegradation].

Three herbicide 2,4-D metabolizing bacterial strains were isolated from three independent soil samples of Estonia. The strains, although belonging to various species, contain 2,4-D degradative plasmids with identical restriction patterns. pEST4001 is a 78 kb conjugative plasmid. All Pseudomonas putida PaW340 2,4-D+ transconjugants obtained a 70 kb plasmid pEST4011 - a deletion derivative of the pEST4001. The restriction patterns of the plasmids mentioned above are considerably different from those of the other 2,4-D plasmids pJP4 and pRC10 reported previously.

2,4-Dichlorophenoxyacetic Acid↗
PubMed1975

[A change in the activity of the beta-phenylpropionic acid conversion enzyme in Escherichia coli under the influence of transmissive plasmids of Salmonella heidelberg].

The activity of the enzyme of the conversion of beta-phenylpropionic acid was studied in the strains of Escherichia coli serotypes 055:K59:H2 and O111:K58:H2 used as recipients via transmission of R- and Col-plasmids by conjugation in vitro. The activity of this enzyme was determined by modified method of Ben Hamida [3]. The wild type strain of Salmonella heidelberg carrying ColIb plasmid and preliminary obtained R1-19 plasmid from E. coli J 5-3 was used as a donor. The activity of the enzyme of conversion of beta-phenylpropionic acid in recombinants carrying R1-19ColIb plasmids was 3-5 times lower as compared with the original recipient. The colour reaction drived under the growth of original bacterial strains in the nutrient broth agar contained beta-phenylpropionic acid at the final concentration of 20 mg% was completely inhibited with that recombinants. The activity of this enzyme in recombinants carrying only R1-19 or ColIb plasmid remains unchanged.

Conjugation, Genetic↗