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Biomedical subjects

A Lageman

Publications and source records attributed to A Lageman.

At least 19 recordsLinked to original sources
PubMed1992

Giant cell granuloma of the lung.

In a 50-year-old man without bronchopulmonary symptoms a round mass lesion close to the hilum of the right lung was detected in a routine chest x-ray and confirmed by computed tomography. Histological examination of two biopsy specimens did not result in a definitive diagnosis. Therefore thoracotomy with enucleation of the focus was performed. The histological picture of the lesion is characterized by connective tissue proliferation, multinucleated giant cells, ossification, localised hemorrhage, deposits of hemosiderin and foci of foam cells. The findings are interpreted as a giant cell granuloma of the lung.

Granuloma, Giant Cell↗
PubMed1988

[Immunohistochemical labeling of immunoglobulin antigens in semithin sections of epoxy resin-embedded tissue using the gold-silver technic].

The silver nitrate variant of the immunohistochemical gold-silver technique is applied to label L-chains within immunoglobulin containing cells in semithin sections of epoxy resin embedded human tonsils. Different possibilities of tissue fixation and of reactivation of antigens are tested. The best results are obtained by paraformaldehyde-sublimate fixation of tissue and potassium iodide processing of sections. This method allows a good preservation of both tissue and antigens and a sensitive labelling of the immunoglobulin antigens by means of the gold-silver technique.

Antigens↗
PubMed1987

[Affinity-histochemical labeling methods using colloidal gold. Overview of developments in the past 15 years].

A survey is given of the development of affinity-histochemical labelling methods using colloidal gold for electron and light microscopy. Historical aspects and trends are discussed and principle methodologic variations are illustrated. A comprehensive compilation of previously published data provides a concise overview of technical variations and applications of the colloidal gold method.

Animals↗
PubMed1985

[Gold-silver technic--a new immunohistochemical method for detecting cell membrane antigens].

The gold-silver technique is presented as a new method for immunohistochemical labelling of cell membrane antigens. At first, membrane antigens of mouse lymphocytes, human leukaemic cells, and experimental brain tumour cells are labelled by immunoglobulin- or anti-immunoglobulin-gold complexes. Secondarily, gold colloid particles are made visible in the light microscope by means of subsequent precipitation of metallic silver around the colloid particles according to the principle of photographic development. The gold-silver reaction can be used after direct or indirect gold-labelling of cell membrane antigens. The technique is suitable for both vital and prefixed cells. Labelling of membrane antigens of vital and prefixed cells, however, results in a different pattern of the gold-silver grains. The gold-silver method is easy and quickly to perform, sensitive and not expensive.

Animals↗
PubMed1981

A variant of the Wiedemann-Beckwith syndrome.

The following combination of findings were established by histological examination of biopsy or autopsy material in an 11-day-old male baby: congenital mesoblastic nephroma, nodular renal blastema, dysgenetic pancreatic cyst, "cytomegaly" of the adrenal cortex, hyperplasia and hypertrophy of the islets of Langerhans, hypoplasia of the thymus and the lymphatic tissue. Since some of these changes constitute the characteristic histological findings in the Wiedemann-Beckwith syndrome and the remainder can be readily reconciled with it, the assignment of this case to this syndrome is discussed. Although the three cardinal symptoms are lacking, the combination of findings are interpreted as a variant of the wiedemann-Beckwith syndrome.

Adrenal Glands↗
PubMed1980

[Preparation and application of hybrid antibodies (author's transl)].

Use of hybrid antibodies with one specificity for IgG and the other for an electron microscopical marker is particularly suited for exact localizing of cell antigens. The preparation of hybrid antibodies of anti-marker and anti-IgG immune sera is described. Different ways of this preparation are discussed, whereby a method of production of hybrid antibodies by use of a combined marker- and IgG-immunoadsorbent is suggested. Application of hybrid antibodies with one specificity for IgG and the other for either ferritin or a plant virus (Erysimum latent virus) is demonstrated on visual labeling of cell membrane antigens. The hybrid antibody technique is preferably assumed to allow a multiple labeling of distinct antigens, scanning electron microscopical examinations and quantitative investigations in the field of immuno electron microscopy.

Animals↗
PubMed1978

[Preparation of hybrid antibodies with mouse IgG and ferritin specifities for the immune electron microscopic detection of cell membrane antigens].

Use of hybrid antibodies with one specifity for mouse-IgG and the other one for ferritin is particularly suitable for immune electron microscopical detection of cell surface antigens. Preparation of antibodies of such kind is described, whereby the method introduced by HMMERLING et al. is varied within some steps of preparation. These variations are discussed here. Activity of produced hybrid antibodies is demonstrated by labeling the THY 1.1. antigen on the cell surface of the thymocytes of the mouse. The advantages of utilizing the hybrid antibodies in comparison with known immune electron microscopical techniques are an excellent location of the antigens, the possibility of using distinct particles for labeling, the application of a multiple labeling, and the fact that investigations by both the transmission and the scanning electron microscope can be carried out by means of the same preparation of hybrid antibodies.

Animals↗
PubMed1977

[A case of central core disease. Electronmicroscopic studies (author's transl)].

A biopsy taken from the right m. gastrocnemius of a sporadic case of central core disease has been investigated by electronmicroscopy. Both longitudinal and cross sections show cores, which are partially in the centre and partially near the sarcolemma of the muscle fibres. These cores are more or less sharply delimited from the intact areas of the muscle fibres. A zone showing a severe destruction of the regularly fibrillar structure is observed in the centre of each core. This destruction zone is surrounded by a transition zone, in which a partial lesion of the fibrillar structure is found whereas the sarcomeric segmentation is preserved. The width of the interfibrillar sarcoplasm is reduced, frequently, however, mitochondria containing cristae of normal structure are observed. The cores are foundlimited by target fibres. Pathogenetically, the damages of z-lines are assumed to be dominant in the formation of the cores.

Child, Preschool↗
PubMed1977

[Electron microscopical findings in the sural nerve of twins with metachromatic leukodystrophy. An attempt to classify the findings according to distinctive phases of the disease (author's transl)].

Sural nerve biopsies taken from a pair of twins with metachromatic leukodystrophy were investigated by electron microscopy. The morphological findings showed distinct alterations. In the first twin the particularity was a large number of granules containing sulfatides. In the second twin, however, the main finding was a damage of myelin sheaths evidently caused by an excessive and morphologically as well as chemically defective myelin formation. According to the grading given by Ulrich the first case corresponds to the stadium one, in which a storage of sulfatides dominates, whereas case two showing a severe damage of myelin sheaths is characteristic of the second stadium of metachromatic leukodystrophy.

Biopsy↗