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Biomedical subjects

A Lakshmy

Publications and source records attributed to A Lakshmy.

13 recordsLinked to original sources
PubMed1999

Screening for Chlamydial infection in women attending STD clinic by polymerase chain reaction, ELISA and cell cytology.

Thirty five female patients with endocervicitis attending STD clinic were studied for the presence of Chlamydial infection by Polymerase Chain Reaction (PCR), Enzyme Immunoassay (EIA) and Cell Cytology. PCR was found to be positive in 54.2% of patients, Enzyme-Linked Immunosorbent Assay (ELISA) in 25.7% of patients, but cell cytology revealed the presence of inclusion bodies only in 3% of the cases, thereby showing that polymerase chain reaction is a better method for detection of Chlamydia trachomatis than EIA and cell cytology.

Adult↗
PubMed1998

An outbreak of post-surgical wound infections due to Mycobacterium abscessus.

An outbreak of post-operative wound infections due to Mycobacterium abscessus is described. During a 5-month period 45 post-surgical patients developed wound infection, manifested by wound breakdown, cellulitis, and discharge and progressing slowly to suppuration and sinus formation. The majority (43/45) had undergone out-patient operations, and 40 had had surgery in the inguinal region. The source of infection was identified as contaminated tap water. A study revealed serious deficiencies in the disinfection and sterilisation techniques employed in the operating theatre (OT), including major defects in the autoclaving machine. The outbreak was controlled after several specific measures were instituted. The patients responded well to treatment with conventional first-line anti-tuberculous drugs administered for 3-8 months. The report highlights the necessity of strict monitoring of disinfection and sterilisation techniques in surgical units and OTs.

Antitubercular Agents↗
PubMed1996

HTLV-I in HIV seropositive Indian blood donors.

One hundred HIV seropositive blood donors were screened for the presence of antibodies to HTLV-I by passive agglutination test from 1994 to 1996. Thirty two percent of the HIV-I seropositive blood donors were found to be positive for HTLV-I (Titre 1:16). Only Six percent of the HIV-seronegative donors were found to be positive for HTLV-I indicating thereby that it should be mandatory to screen all the blood samples for transfusion for HTLV-I antibodies also.

Adult↗
PubMed1993

Neisseria meningitidis: serotyping and subtyping by whole cell ELISA.

Twenty strains of Neisseria meningitidis isolated from clinically diagnosed cases of meningococcal disease were subjected to serogrouping, employing slide agglutination followed by serotyping and serosubtyping by whole cell ELISA using monoclonal typing antisera. All isolates were from sporadic cases of meningitis during a period of two years from various hospitals in Delhi. All 20 isolates were grouped as serogroup A and typed as serotype 4, except one strain which was untypable. On serosubtyping the isolates were found to belong to P1.9 (7 strains) followed by P1.1 (5), P1.9 (2), P1.16,1 (2), P1.6,10 (2), P1.10,7,1 (1) and non-subtypable (1).

Animals↗
PubMed1992

Streptococcal throat carriage in school children with special reference to seasonal incidence.

A number of studies on throat carriage of beta hemolytic streptococci (BHS) carried out during the years 1972-90 in urban and rural school children from low socioeconomic groups in the age group of 5-15 years in and around Delhi showed an overall carriage rate of BHS varying from 12.2%-64.3% depending upon the season and number of swabs taken. Group A was found to be the most predominant serological group (31.1%-62.6%). The T-typability was found to be 98.2%. The most prevalent T-patterns observed during 1972-78 study were 3/13/B3264 followed by 5/11/12/27/44. A significant difference was observed in the prevalence of T-patterns during the study of 2,034 children from 1979-83 and 3,094 children from 1984-90. When the most prevalent T-patterns were found to be 5/11/12/27/44 followed by 3/13/B3264. The study of the school children from 1972-90 showed the isolation of BHS as well as significant predominance of GAS (p < 0.001) in winter months than summer months. There was no difference in the distribution of carriage of BHS and GAS amongst rural or urban school children. Since RF/RHD are illnesses which were often encountered in school children among socially and economically disadvantaged populations stronger support for streptococcal surveillance programs should be encouraged.

Adolescent↗
PubMed1991

Comparison of enzyme linked immunosorbent assay and indirect haemagglutination test in serologic diagnosis of toxoplasmosis.

The antibody response against Toxoplasma gondii was evaluated by ELISA and indirect haemagglutination test (IHA) in 100 antenatal cases with bad obstetric history. 30 per cent of the cases were found to be positive by the IgM ELISA test and 22 per cent were positive by the IHA test. ELISA was found to be a more specific, reliable and easy test for screening of antenatal cases.

Acute Disease↗