Biomedical subjects

A Le Gô

Publications and source records attributed to A Le Gô.

13 recordsLinked to original sources

PubMed1978

[Lymphocyte subpopulations in adjuvant arthritis of rats. Effects of corticoids and gamma irradiation].

Experimental model of human chronic inflammatory arthritis, adjuvant arthritis may be induced only in several strains of inbred Rats: it is well developed in LEW and practically absent in WAG. After adjuvant injection, the PHA-stimulable lymphocytes subpopulation quite disappears from the blood, if polyarthritis is well developed. These cells are probably capted in the tissues implicated in immunological conflict. On the contrary, the ConA-stimulable subpopulation is enhanced in both strains after adjuvant injection, earlier and more intense in WAG than in LEW and that phenomenon is probably linked to a stimulation of suppressor T lymphocytes. Treatment with prednisone or gamma rays inhibits partially and delays the appearance of arthritis in LEW, acting essentially on ConA-stimulable subpopulation.

Adjuvants, Immunologic↗

PubMed1976

Immunofluorescence of synovial membrane multifactorial analysis of the results.

Synovial membrane taken by needle biopsy from the knee joint of 61 patients with various rheumatic diseases were studied using immunofluorescence methods. Staining techniques and their controls were detailed. Classical statistical tests and principal components multifactorial analysis of the data emphasized some differences between the pathological groups. Connective tissue diseases seemed to be characterized by plasma cells fluorescence and mixed immunoglobulins and complement deposits. These were mostly localized to extracellular spaces in sero-positive rheumatoid arthritis and to blood vessels in sero-negative rheumatoid arthritis and systemic lupus erythematosus. On the contrary, isolated immunoglobulins without complement were mostly found in the other inflammatory arthritis, while negative results were obtained in non inflammatory arthropathy. Immunoglobulin classes did not seem to have any diagnostic value. On the contrary, rheumatoid factor was specific for rheumatoid arthritis, whatever the serological pattern was, and it was particularly frequent in patients suffering from rheumatoid arthritis associated with a Sj5AOGREN SYNDROME. A strict relationship between classical histological findings and immunofluorescence results was not always found; so, immunological methods can be aquivocal.

Arthritis↗

PubMed1975

Hyperbasophilic immunoblasts in circulating blood in chronic inflammatory rheumatic and collagen diseases.

The number of large circulating hyperbasophilic mononuclear cells - referred to as hyperbasophilic immunoblasts (HBI)- is often increased in collagen disease and rheumatoid arthritis (RA) and grossly reflects the degree of disease activity. In contrast, in psoriatic arthropathy the percentage of (HBI) is within the normal range. HBI are mainly involved in immune reactions and may provide a valuable routine test for the assessment of the latter in disease states and for the predicition of relapse in chronic collagen diseases. Immunofluorescent techniques applied to samples from active autoimmune diseases have shown that a number of HBI are Ig-producing B-blasts. Moreover, in a few cases these intracytoplasmic immunoglobulins exhibited a rheumatoid factor-like activity, a finding which promises to yield additional information on the immunopathogenesis of RA.

Antibody-Producing Cells↗

PubMed1975

Soluble complexes and antiglobulin factors detection by immunofluorescence and immunoadsorption in rheumatoid sera.

In this report is was demonstrated that in rheumatoid factors (RF) positive sera, 19 S IgM rheumatoid factor can form soluble complexes with different proteins (IgG, albumin) of sera. In these complexes the antiglobulin activity of IgM is not inhibited. When immunofluorescence and immunoadsorption procedures are used for the detection of antiglobulin activities of rheumatoid sera, the proteins which are bound to IgM rheumatoid factor, even if they are devoid of any antiglobulin character, may be revealed simultaneously with IgM. Moreover in some cases the detection of IgM may be hindered, while the linked proteins remain detectable. In these conditions, these complexes in RF positive sera may give false negative results for IgM rheumatoid factor, and may give rise to artefactual appearance of IgG and other proteins (albumin antiglobulin-like activities. This paper points out that before investigating IgG and IgA antiglobulin activities, IgM rheumatoid factor should be previously eliminated, for example by immunoadsorption.

Antigen-Antibody Complex↗

PubMed1974