Visualization of intracellular calcium during necrotaxis.
Granulocytes are known to exhibit positive chemotaxis towards erythrocytes destroyed by laser irradiation (necrotaxis). A computerized method for visualizing intracellular calcium concentration in granulocytes during necrotaxis has been developed. Successive bursts of fluorescence were observed: 1 degree) about 10 s after the creation of the necrotactic source; 2 degrees) when the granulocyte touched the target.
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