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A Lepoint

Publications and source records attributed to A Lepoint.

33 records · Page 2Linked to original sources
PubMed1977

Selection of Ehrlich tumor cells in different phases of the cell cycle by ficoll gradient centrifugation.

Mouse Ehrlich ascites tumor cells were centrifuged at low speed on a linear Ficoll gradient. Cells from different fractions of the gradient were collected separately and analysed by cytological and cytochemical methods (DNA content, mean volume and number of cells). Nearly pure populations of cells in G1 or S were selected. A heterogeneous population containing 70% G2 and mitotic cells was also isolated. No ultrastructural alterations were detected in the cells after centrifugation. Selected G1 cells were cultured in vitro, their kinetic parameters were measured and compared with those of the original population. No difference was observed as far as the duration of the cycle of these cells is concerned.

Animals↗
PubMed1975

Mode of action of amphotericin B on chick embryo fibroblasts and on mouse Ehrlich tumour cells: a cytological and cytochemical analysis.

Chick embryo fibroblasts cultivated in vitro and Ehrlich ascites tumor cells (in vivo or in vitro) have been treated with amphotericin B. Cell multiplication is strongly inhibited. Large clear zones appear in the fibroblast nucleoi (phase-contrast and electron-microscope observations). Many treated fibroblasts and tumour cells have a high DNA content (pre-mitotic or polyploid level; measurements by cytophotometry). However, the RNA content (cytophotometry) and the total protein content (cytophotometry and micro-interferometry) are relatively low in the tumour cells. As shown by autoradiography, DNA synthesis is active but RNA synthesis and, in some cases, protein synthesis are inhibited. Due to this unbalanced growth, the cells cannot divide.

Amphotericin B↗