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A Liss

Publications and source records attributed to A Liss.

27 records · Page 2Linked to original sources
PubMed1974

Intracellular replication of mycoplasmavirus MVL51.

The intracellular replication of MVL51, a group L1 mycoplasmavirus, was investigated. The single-stranded parental DNA was found to enter the cell and become converted to double-stranded DNA. This replicated to yield additional double-stranded DNA molecules. The parental viral DNA was found to leave the replication complex and become associated with large molecular weight DNA not involved with viral replication. Progeny viral DNA formed from the double-stranded DNA and an intracellular accumulation of virus chromosome size DNA was observed. The interpretation of this data and a suggested model for the viral replication are discussed and compared to viral DNA replication models for other single-stranded DNA viruses.

Acholeplasma laidlawii↗
PubMed1972

Transfection mediated by Mycoplasmatales viral DNA.

DNA isolated from Mycoplasmatales viruses MVL51 and MVGs51 was infectious when mixed with Acholeplasma laidlawii BN1-Na1(R) cells. Infectivity was destroyed by deoxyribonuclease but not by ribonuclease, Pronase, or specific antiserum to the virus. Host mycoplasma cells were only competent for transfection during late-log growth phase. The rates of the establishment of DNase insensitivity of viral DNA transfectants were similar to those of bacteriophage systems. The dose-response curve for transfection suggested that an average of six molecules of DNA must interact with a cell in order to produce one infectious center. Mycoplasmatales virus DNA exhibited a low efficiency of infection; one infectious center required 4 x 10(5) virus equivalents of DNA.

Bacteriophages↗
PubMed1971

Isolation of Mycoplasmatales viruses and characterization of MVL1, MVL52, and MVG51.

Eleven Mycoplasmatales viruses are now known. Burst size, burst time, sensitivity to ultraviolet and the host range of three viruses that were studied are different. However, all three are naked, rod-shaped particles of similar size. Plaque morphology and the isolation of immune cells suggest that both virulent and nonvirulent infections are possible.

Bacteriophages↗
PubMedDate not supplied

Spiroplasmal viruses: group 1 characteristics.

Virus-like particles of three morphologic groups have been detected by electron microscopy in 90% of spiroplasma strains. In this study, virus-like particles of group 1 (SpV1)--unenveloped rods 230-280 nm by 10-15 nm--were found in 575 of spiroplasmas. SpV1 occurred spontaneously in large numbers in cultures of strains B (corn stunt), G1 (tulip tree flower), BC3 (honeybee), and KC3 (honeybee), respectively; the viruses were isolated and assayed as plaque-forming units, with several of the same strains used as indicators. No virus plaqued on its strain of origin, and all except one (SpV1/BC3) plaqued best on strain BC3. Host range and yields of progeny virus differed. All isolates were identical in morphology, size, and buoyant densities; contained DNA; formed turbid 1.5 to 5.0-mm plaques; produced nonlytic infections and similar one-step growth curves; were inactivated by a single antiserum; had similar kinetics of adsorption and heat inactivation; and demonstrated similar patterns of resistance and sensitivity to a variety of physical and chemical agents, as well as an inability to infect resistant host mutants. SPV1 isolates from diverse host strains apparently share many physicochemical and biological properties, but are not identical in all biological respects.

Adsorption↗