Biomedical subjects
Publications and source records attributed to A M Chistiakova.
A new subclass of high density lipoproteins designated as HLD2c was found in the human blood plasma of newborn by analytic ultracentrifugation in the flotation rate interval Fo1,20 8.0--12.0. HDL2c was isolated from the total HDL fraction of newborn by preparative ultracentrifugation at the density 1.071.
Explore the source record for details and available documents.
A number of biologically active substances, i. e. main protamine proteins and histones, EDTA, lysozyme, methacyl and pentoxyl was studied with respect to their effect on the levels of the minimum inhibitory concentrations of antibiotics against E. coli and Staph. aureus and transfer of resistence to chloramphenicol in E. coli on conjugation. It was shown that the above substances lowered the minimum inhibitory concentrations of the antibiotics 2--10 times when added to the culture simultaneously with the latter. The results varied depending on the strain and the resistance nature. Marked inhibition of transfer of resistance to chloramphenicol in the presence of the main proteins and EDTA was found.
Content of beta- and pre-beta-lipoprotein fractions, total cholesterol and triglycerides was distinctly lower in blood serum of infant than in maternal one. In serum of funicular blood content of free fatty acids was 2.5-fold higher than in maternal blood serum; in erythrocytes of infant content of cholesterol was found to be higher by 40%, as compared with maternal erythrocytes. In blood serum of infant content of free cholesterol amounted to 28% of the total cholesterol at the same time in maternal blood it was equal to 37%. The highest proportion of cholesterol was found in alpha-lipoproteins of infant, in mother it was observed in beta-lipoproteins. In blood of infant and mother the same types of lipoproteins were present but their quantitative ratio was different. The main lipoproteins of infant were shown to be alpha-lipoproteins. Relationship between the content of cholesterol in pre-beta-lipoproteins (correlation coefficient 0.64) was noted in blood of mother and infant.
Explore the source record for details and available documents.
On the basis of data obtained in studies on the accumulation of cholesterol in erythrocytes of newlorns, the hypothesis is advanced on the deficiency of lecithin-cholesterol-acetyl transferase (LCAT) in the newborn children. A decrease in the rate of the LCAT-reaction was shown in funic blood using the specially developed system "LP-infranatante", the constituents of which were isolated from blood plasma of both newborns and of their mothers by ultracentrifugation at a density 1.21 g/ml and 40.000 rpm. The following factors were considered as possible determinants of the decrease in the rate of cholesterol esterification in blood plasma of newborns: 1) insufficiency in LCAT, 2) alteration in the composition of the substrate lipoproteins (lipoproteins of high density).