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Biomedical subjects

A M Collins

Publications and source records attributed to A M Collins.

46 records · Page 3Linked to original sources
PubMed1985

Fetal lung hypoplasia associated with maternal smoking: a morphometric analysis.

Our recently reported animal (rat) model of maternal cigarette smoke exposure during pregnancy is characterized by fetal growth retardation and lung hypoplasia. We have further defined the fetal lungs using quantitative histologic techniques. Compared to controls, lung volume at term is reduced in the experimental animals (0.28 versus 0.33 ml, p less than 0.005). Saccules (fetal "alveoli") are reduced in number (3 X 10(6) versus 5.5 X 10(6), p less than 0.005) and increased in size (average saccular volume: 35 X 10(-9) versus 21 X 10(-9) ml, p less than 0.025). These changes in size and number are consequences of reduced formation of saccule partitions (septal crests) in the experimental lungs (volume density: 0.013 versus 0.018, p less than 0.025). Internal surface area is decreased in the hypoplastic lungs (161 versus 198 cm2, p less than 0.001). The total length of parenchymal elastic tissue is diminished (224 versus 354 M, p less than 0.05). In short the hypoplastic lungs contain fewer, larger saccules and the surface potentially available for gas exchange is reduced. These results show that maternal smoking in rats adversely modifies fetal lung growth. If these observations are applicable to humans, then reduced lung growth in children of smoking mothers may begin antenatally.

Animals↗
PubMed1980

The effect of nitrogen dioxide on tracheal uptake and transport of horseradish peroxidase in the guinea pig.

The effect of 5 and 15 ppm of nitrogen dioxide (NO2) on airway transepithelial permeability to horseradish peroxidase (HRP) (molecular weight, approximately 40,000 daltons) was studied in the guinea pig. Age- and sex-matched guinea pigs (n = 34) were exposed to NO2 for 2 and for 14 days. In the control guinea pigs, 0.05% of the intratracheally instilled dosage HRP was present in the plasma within 10 min, as detected by radioimmunoassay, and increased thereafter at amean rate of 0.0016%/min. After exposure to NO2, there was a significant increase in the rate of plasma HRP accumulation (p < 0.05) with the maximal increase detected in the group exposed to 15 ppm of NO2 for 2 days. The morphologic correlates of increased permeability to HRP appear to be (a) an increase in pinocytotic activity within the hyperplastic globlet cells, and (b) transjunctional transport by leaky tight junctions that was noted only in animals exposed to 15 ppm for 14 days (p < 0.01). Our data suggests that pinocytotic vesicular transport of proteins in the secretory cells of the airway may be an important mechanism for the movement of exogenous nonlipid soluble macromolecules across the epithelial barrier.

Animals↗
PubMedDate not supplied

Quantitative trait loci influencing honeybee alarm pheromone levels.

Quantitative trait loci (QTL) mapping procedures were used to identify loci that influence the levels of alarm pheromones found in the stinging apparatus of worker honeybees. An F1 queen was produced from a cross between a queen of European origin and a drone descended from an African subspecies. Haploid drones from the hybrid queen were individually backcrossed to European queens to produce 172 colonies. Samples of stings were taken from backcross workers of these colonies. Alarm pheromone levels were determined by gas chromatography. RAPD markers were scored from the haploid drone fathers of these colonies. The multiple-QTL model (MQM) of MapQTL was used to identify QTLs that influence the levels of four alarm pheromone components. Seven independent, potential QTLs were identified with LOD scores greater than two, and one at LOD 1.88. We identified one QTL for n-decyl acetate, three for n-octanol, four for isopentyl acetate, and one for hexyl acetate. One region of linkage group XI shows a strong influence on body size and the levels of three alarm pheromone components. This locus explained 40% of the variance for the amount of n-decyl acetate (LOD 6.57). In general, the QTLs influencing alarm pheromone levels were independent of previously identified loci that influenced the stinging behavior of these colonies. The only exception was a potential locus influencing levels of n-octanol, which was inversely correlated with stinging behavior.

1-Octanol↗
PubMedDate not supplied

Partial cytochrome b sequences for six Hymenoptera of the eastern United States.

Mitochondrial DNA (mtDNA) haplotypes have been commonly used to determine honeybee subspecies relationships. To see if these markers would also be useful for comparisons of other Hymenoptera, we collected workers of six local species: Vespa crabro, the European hornet; Bombus impatiens, a bumblebee; Vespula germanica, the German yellow jacket; Polistes fuscatus, a paper wasp; Halictus ligatus, an alkali bee; and an unspecified Megachile, a leafcutting bee. MtDNA was isolated and digested with six endonucleases (AvaI, BglII, EcoRI, HindIII, HinfI, XbaI). The digested DNA was electrophoresed and visualized on agarose gels with comparison to a standard fragment marker and similarly treated honeybee mtDNA. The fragments obtained were also purified and sequenced. Phylogenetic relationships between six wasp and bee species, Apis mellifera, and several other similar aculeate Hymenoptera were determined. Newly defined DNA sequences were posted to GenBank (AF281169-AF281174).

Animals↗
PubMedDate not supplied

Perceived stress and stress projected into the spontaneous storytelling of two groups of fourth grade children.

The purpose of this descriptive correlation study was to describe stress levels in fourth grade children from two geographic locations: an inner city recreation club and a suburban parochial school. The use of spontaneous storytelling and a life events questionnaire provided information about the stress being experienced by the children. This article presents story themes and stressful life events of interest to adult caregivers helping children cope with stressful experiences.

Adaptation, Psychological↗
PubMedDate not supplied

Use of a family caregiving model to articulate the role of the public health nurse in infant mental health promotion.

Public health nurses (PHNs) have dedicated their efforts to promoting the health and welfare of mothers, infants, and children, receiving little recognition for the mental health promotion benefits they provide to these populations. The purpose of this article is to describe the contributions of PHNs to the promotion of mental health in infants and their families. The Family Caregiving Model (Zerwekh, 1991) is used to identify the strategies used by PHNs to promote infant mental health within vulnerable families. Using a case study format, the authors describe these strategies and the competencies and skills needed by PHNs to assess for cues in infants and their parents that may indicate the potential for unsuccessful attachments.

Adult↗