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A M Drapier

Publications and source records attributed to A M Drapier.

24 records · Page 2Linked to original sources
PubMed1986

Paucity of phosphorylcholine-specific clones in B cells expressing the VHT15 gene product.

The aim of this work was to study the cellular basis of the phenomenon of clonal dominance. To this end we analyzed two collections of BALB/c and C.B20 hybridomas that we selected on the basis of the expression of the VHT15 gene product independently from their antigen specificity. Our study demonstrates that none of the 28 BALB/c and only 2 of the 29 C.B20 hybridomas obtained have variable regions that bind PC. We conclude therefore that the domination of the immune response to PC by particular variable regions cannot be due to the establishment of clonal dominance prior to immunization.

Animals↗
PubMed1986

Differential expression of sets of highly homologous variable region gene products in selected and preimmune repertoires of inbred mouse strains.

Using mAb that selectively recognize the various allelic forms of the VHT15 and Vk21D-E genes' products, we analyzed the influence of VH and Vk polymorphism on the probability of expression of these gene segments. Our data show that the frequency to which the VHT15 gene product becomes available in the preimmune repertoire is strongly influenced by the polymorphism of the relevant structural gene, suggesting therefore that VH genes cannot be randomly used in the various strains. Contrary to this, the frequency of Vk21D-E+ clones is similar in all mouse strains tested, and in all cases is higher than the frequency of VHT15 clones. This observation strongly suggests that Vk genes can be randomly expressed, and/or that their number is lower than that of their VH counterpart. Finally, analysis of the specificity associated to the expression of the VHT15 segment revealed that VH polymorphism strongly influences not only the probability of expression of each V gene, but also the specificity of the antibodies on which these VH genes are used.

Animals↗