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A M Flores

Publications and source records attributed to A M Flores.

8 recordsLinked to original sources
PubMed1998

E2F-3 accumulation is regulated by polypeptide stability.

E2F is a complex family of transcription factors which appears to regulate the transcription of genes required for the S phase of the mammalian cell cycle. In the present work, we have examined the mechanisms regulating E2F-3 accumulation in mouse fibroblasts. We have determined that E2F-3 DNA binding activity is restricted to the G1/S transition and S phase in both normal BALB/c-3T3 fibroblasts and in an SV40 virus-transformed BALB/c-3T3 derivative. Immunoblot analysis indicates that G0 and G1 cells have little or no E2F-3 polypeptide and that the increase in the DNA binding activity of E2F-3 at the G1/S boundary is reflected by an increase in total E2F-3 protein. In contrast to the E2F-3 polypeptide, RNAse protection assays demonstrate that the E2F-3 mRNA is clearly present in G0 and G1 cells. Finally, pulse/chase experiments indicate that the half-life of E2F-3 is approximately 40-fold greater in cells blocked in S phase relative to asynchronously growing cells. Together, these results indicate that the accumulation E2F-3 at S phase may be regulated, at least in part, at the level of protein stability.

3T3 Cells↗
PubMed1990

Cardiovascular, pulmonary, and cancer rehabilitation. 2. Cardiovascular considerations in the rehabilitation of the disabled.

This self-directed learning module addresses core concepts in the application of principles of cardiac rehabilitation for the physically disabled population. It is part of the chapter on cardiovascular, pulmonary, and cancer rehabilitation for the Self-Directed Medical Knowledge Program Study Guide for practitioners and trainees in physical medicine and rehabilitation. This section contains general and athletic conditioning programs for the disabled, rehabilitation of the disabled with coexisting cardiac disease (including modified exercise tolerance testing, cardiac demands of rehabilitation programs, and cardiac precautions), and peripheral circulatory dysfunction (arterial, venous, and lymphatic).

Bibliographies as Topic↗
PubMed1990

Cardiovascular, pulmonary, and cancer rehabilitation. 1. General principles of cardiovascular rehabilitation.

This self-directed learning module highlights advances in this topic area. It is part of the chapter on cardiovascular, pulmonary, and cancer rehabilitation for the Self-Directed Medical Knowledge Program Study Guide for practitioners and trainees in Physical Medicine and Rehabilitation. This section reviews the anatomy of the heart, physiology of exercise training, pathophysiology and clinical aspects of cardiac conditions frequently encountered in rehabilitation, cardiac evaluation techniques, physical conditioning for normal persons, and rehabilitation of the patient with heart disease. Advances that are covered in this section include indications for cardiac rehabilitation following coronary artery bypass graft compared with percutaneous transluminal coronary angioplasty and exercise testing and physical conditioning of patients on long-term beta-adrenergic blockade or with severe left ventricular dysfunction.

Bibliographies as Topic↗