Biomedical subjects

A M Kilbourn

Publications and source records attributed to A M Kilbourn.

3 recordsLinked to original sources

PubMed2001

Serum antigen 85 levels in adjunct testing for active mycobacterial infections in orangutans.

Diagnosis of active mycobacterial disease in orangutans (Pongo pygmaeus) has been impeded by high levels of non-specific intradermal skin test reactivity to mycobacterial antigens. This may be due in part to cross reactivity between antigens, tuberculin concentrations used or other species-specific factors. Antigen 85 (Ag85) complex proteins are major secretory products of actively growing mycobacteria, and measurement of serum Ag85 could provide a method for determining active mycobacterial infections that was not dependent on host immunity. Serum Ag85 was measured by dot-immunobinding assay using monoclonal anti-Ag85, purified Ag85 standard and enhanced chemiluminescence technology in coded serum samples from 14 captive orangutans from a zoo in Colorado, 15 semi-captive orangutans in Malaysia, and 19 free-ranging wild orangutans in Malaysia. Orangutans from Colorado (USA) were culture negative for Mycobacterium tuberculosis and M. avium, although all had laboratory suspicion or evidence of mycobacterial infection; median serum Ag85 was 10 microU/ml (range, <0.25-630 microU/ml). Of the semi-captive orangutans, six were skin test reactive and two were culture positive for M. avium on necropsy. Median serum Ag85 for this group was 1,880 microU/ml (0.75-7,000 microU/ml), significantly higher than that of Colorado zoo or free-ranging Malaysian orangutans. Median serum Ag85 in the latter group was 125 microU/ml (range, 0.75-2,500 microU/ml). These data suggest that suggest that additional studies using more specific reagents and more samples from animals of known status are appropriate.

Animals↗

PubMed2000

Conservation medicine.

The Field Veterinary Program (FVP) of the Wildlife Conservation Society (WCS) was created in 1989 to combat the wildlife disease and health problems that increasingly complicate the process of wildlife conservation. The FVP provides veterinary services for the more than 300 WCS conservation projects located in more than 50 countries around the world. Most of these projects are in tropical regions and many have a wildlife/domestic livestock component. Wildlife health care provided by the FVP staff includes (1) identifying critical health factors; (2) monitoring health status; (3) crisis intervention; (4) developing and applying new technologies; (5) animal handling and welfare concerns; and (6) training. Additionally, the staff of the FVP give expert advice to many governmental and non-governmental agencies that are involved in setting policies directly related to wildlife health and conservation issues. In this paper, two FVP projects are presented as examples of studies that have increased our understanding of the role wildlife diseases may play in the health of livestock and human populations, as well as the role humans and livestock may play in the health of wildlife populations. Examples of the collaborative work between the FVP staff and scientists from many disciplines (e.g., acarologists, mycobacterium experts, ecologists, and biologists) are also presented.

Animals↗

PubMedDate not supplied

Sylvatic transmission of arboviruses among Bornean orangutans.

Wild populations of nonhuman primates live in regions of sylvatic arbovirus transmission. To assess the status of arbovirus transmission in Bornean forests and the susceptibility of wild orangutans to arboviral infection, blood samples of wild orangutans, semi-captive orangutans, and humans were examined. Samples were tested by plaque reduction neutralization test for antibodies to viruses representing three families (Flaviviridae, Alphaviridae, and Bunyaviridae), including dengue-2, Japanese encephalitis, Zika, Langat, Tembusu, Sindbis, Chikungunya, and Batai viruses. Both wild and semi-captive orangutan groups as well as local human populations showed serologic evidence of arbovirus infection. The presence of neutralizing antibodies among wild orangutans strongly suggests the existence of sylvatic cycles for dengue, Japanese encephalitis, and sindbis viruses in North Borneo. The present study demonstrates that orangutans are susceptible to arboviralinfections in the wild, although the impact of arboviral infections on this endangered ape remain unknown.

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