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A M Pimenov

Publications and source records attributed to A M Pimenov.

27 records · Page 2Linked to original sources
PubMed1986

Simultaneous separation of ribonucleotides, nucleosides and nitrogen bases by ion-pair reversed-phase high-performance liquid chromatography on columns with radial compression.

A method is described for the simultaneous analysis of twelve major nucleotides, certain biochemically essential nucleosides and nitrogen bases. The separation was achieved using an ion-pair reversed-phase high-performance liquid chromatographic (HPLC) system with a radially compressed NovaPak C18 cartridge and tetrabutylammonium phosphate (TBA) as a mobile phase modifier under acetonitrile gradient conditions. The effects of TBA and acetonitrile on the retention of major nucleotides were studied. The retention of nucleotides increased significantly with an increase in the TBA concentration from 0 to 2 mM and remained constant up to 5 mM TBA. The logarithm of the capacity factor decreased in proportion to the concentration of acetonitrile in the mobile phase. The separation of nitrogen bases, nucleosides and some of their deoxy forms was performed on an isocratic ion-pair reversed-phase HPLC system with pentanesulphonic and heptanesulphonic acid using a mu Bondapak C18 column. The described chromatographic procedures were applied to the separation of various biological extracts.

Animals↗
PubMed1986

[Destruction of muscle tissue and purine compound metabolism in hereditary muscular dystrophy].

The distribution of purine compounds in the skeletal muscles of the anterior and posterior limbs of 129/Re mice with hereditary muscular dystrophy (HMD) was investigated in a comparative study. The results revealed unidirectional metabolic disorders in both groups of muscles which was manifested in a quantitative redistribution of phosphorus-containing purine components and a decrease in the pool of adenylates in muscle tissue. Elevated concentrations of purine metabolites (inosine, hypoxanthine, xanthine, and uric acid) indicated an augmented metabolism of purines in this abnormality. Impairment of metabolic transformations of purines in the muscle tissue in HMD may be one of the factors responsible for the development of the pathological process in the muscle in the given disease.

Adenine Nucleotides↗
PubMedDate not supplied

[Features of purine compound metabolism in hepatoma 22, mouse liver and erythrocytes during tumor development].

During development of transmitted solid hepatoma 22 pools of purine metabolites were studied as well as the rate of 3H-hypoxanthine and inosine incorporation was measured in acid soluble fraction of liver tissue and erythrocytes of mice C3HA as well as of the tumor tissue. Specific alterations in metabolism of adenine and guanine nucleotides and of their derivatives were detected at various steps of the hepatoma development in the tissues studied. Specific interaction of the hepatoma cells with host tissues appears to be realized via the pool of purine nucleotides.

Adenine Nucleotides↗
PubMedDate not supplied

[Optimization of separation of purine and pyrimidine compounds using ion-pair reversed-phase high performance chromatography (HPLC)].

Utilization of ion-air reagents in a reversed-phase chromatographic system allows solving a number of problems related to the separation of purine and pyrimidine derivatives. Simultaneous analysis of nucleotides, nucleosides and their bases was carried out by acetonitrile gradient elution using tetrabutyl ammonium phosphate as a counterion in the mobile phase. Besides, optimal conditions were selected for isocratic separation of adenine nucleotides and their metabolites. Furthermore, isocratic separation of certain purines and pyrimidines was achieved by modifying the stationary C18-phase with pentane- and heptane sulphonic acids.

Chromatography, High Pressure Liquid↗
PubMedDate not supplied

[Determination of purine and pyrimidine derivatives in acid-soluble fractions of organs and tissues using high-performance reverse-phase ion-pair chromatography].

Simultaneous separation of the main ribonucleotides, nucleosides and bases was carried out using tetrabutyl ammonium phosphate (TBA) hetaerons with a reversed-phase (C18) packing material. Chromatographic properties of purine and pyrimidine metabolites in radially compressed NovaPak C18 system was studied at various concentrations of acetonitrile and TBA in the mobile phase. The optimal conditions for gradient separation of nucleotides, nucleosides and bases were evaluated. The procedure was used for analysis of blood from healthy persons and patients with acute myocardial infarction.

Animals↗
PubMedDate not supplied

[Metabolic pool of purine and pyrimidine derivatives in the palatine tonsil of patients with chronic tonsillitis].

Tonsils from 10 patients (18-40 years old), with chronic tonsillitis were studied by means of histological methods as well as by high pressure liquid chromatography. In 5 patients with decompensated forms of the disease, accompanied by heart impairments during 1-3 years, only slight alterations in epithelial and follicular parts of tonsils were observed, while pronounced destruction of tonsil cells was detected in other 5 patients suffering from repeated anginas. The following purine and pyrimidine derivatives were identified in faucial tonsil tissue of all the patients with chronic tonsillitis studied: adenine, guanine, adenosine, guanosine, inosine and uridine. Distinct increase in content of these derivatives as well as occurrence of xanthine and uric acid (non-identified in the first group of patients with chronic tonsillitis) was noted in the patients with maximal destruction of faucial tonsil cells.

Adolescent↗
PubMedDate not supplied

[Radiation-protective effectiveness of lycopene].

Radioprotective action of natural carotenoid Lycopene in mice during exposure to lethal dose of 6.5 Gy has been shown. Lycopene was found to be two-fold more effective radioprotector than beta-carotene at the same conditions. This correlates with the difference in their antioxidant activity. Lycopene also exhibits antimutagenic effect resulting in increased survival of Drosophila melanogaster chrysalis under exposure to 40 Gy gamma-radiation.

Animals↗