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Biomedical subjects

A M Poverennyĭ

Publications and source records attributed to A M Poverennyĭ.

At least 19 recordsLinked to original sources
PubMed2004

[Characteristics of a clinical course of immune cytopenias with a high titer of autoantibodies to the microsomal antigen of the thyroid gland].

AIM: To ascertain the role of high antibodies (Ab) titers to microsomal antigen (MA) of the thyroid in clinical manifestations of cytopenic syndromes. MATERIAL AND METHODS: Clinical data are presented on 144 patients with depressed hemopoiesis having different levels of thyroid Aab. RESULTS: When the titer was significantly elevated (41% patients with immune cytopenias), hematological malignancies run with recurrences, remission is rare. Treatment of the thyroid pathology improves hematological indices in some cases though does not reduce titers of Ab thyroid MA. CONCLUSION: Detection of autoimmune pathology of the thyroid is necessary not only for specification of the concurrent process diagnosis but also for initiation of adequate combined treatment.

Adolescent↗
PubMed2003

[Autoantibodies to the thyroid gland in hemoblastoses and cytopenias].

AIM: To study the level of thyroid antibodies (TAB) in blood diseases. MATERIAL AND METHODS: TAB levels dynamics was studied in 413 patients with hematological diseases. RESULTS: Increased incidence of cases with high Ab titer to thyroid microsomal antigen was found. High and moderate titers were revealed in 33% of all examinees, including 41%, 22%, 32% and 29% among patients with immune cytopenia, chronic lymphoid leukemia, generalized mature-cell lymphoma and myeloproliferative diseases, respectively. These high and moderate titers were rather stable. In 15% patients Ab were elevated insignificantly, this rise being unstable. CONCLUSION: Stable high titers of antibodies to thyroid antigens point to increased incidence rates of concomitant autoimmune hyroiditis in patients with blood diseases.

Autoantibodies↗
PubMed1996

[Changes in plasma levels of lipid peroxidation products and myoglobin in patients with malignant tumors in the early postoperative period].

Changes in the levels of free myoglobin and lipid peroxidation products (hydroperoxides and malonic dialdehyde) were studied in cancer patients. Their baseline levels were found to be higher in patients with cancer of the lung, large bowel, and rectum than those in controls, which indicates higher lipid peroxidation, which led to the destabilization of cellular membranes and seems to cause baseline hypermyoglobinemia. There was a drastic rise in the levels of free myoglobin in the first 24 postoperative hours. The duration and severity of hypermyoglobinemia were much higher in the complicated than uncomplicated postoperative period. There was a less rise in malonic dialdehyde concentrations, whose peak was observed on days 1-2 postsurgery. The content of hydroperoxides decreased in the first 24 postoperative hours and began gradually increased from day 2. The correlation between the concentrations of myoglobin and malonic dialdehyde suggests that there is an association between hypermyoglobinemia and the status of cellular membranes.

Adult↗
PubMed1995

[Detection of Chlamydia trachomatis and Ureaplasma urealyticum by hybridization analysis using DNA-Pt((dien)Cl)Cl-probes and PCR].

A simple method for detecting pathogenic microorganisms in clinical samples has been developed. This method is based on identification of specific nucleotide sequences by Pt[(dien)Cl] Cl-labelled DNA probes and simultaneous immunochemical control of total DNA content in each clinical sample. Such control is provided by a simple semiquantitative enzyme-linked immunoassay using antibodies to denatured DNA; its implementation requires the same procedures, materials and solutions used in the hybridization analysis. The information about the DNA content in the sample is necessary for adequate interpretation of results of hybridization analysis. Pt[(dien)Cl]Cl for the probe and affinity antibodies to DNA-Pt[(dien)Cl] Cl and rabbit immunoglobulin antibodies conjugated to phosphatase for DNA hybrid detection were used. The results of hybridization analysis show a good correlation with those of the PCR-test. The method is simple, relatively inexpensive and fit for population monitoring.

Chlamydia Infections↗
PubMed1994

[Use of diethylenetriaminochloroplatinum in hybridization analysis of specific nucleic acid sequences].

Monofunctional platinum compound [Pt(dien)Cl] Cl and high-affinity antibodies against DNA-[Pt(dien)Cl]Cl were suggested for non-radioactive hybridisation analysis of DNA. The simple labelling procedure was based on mixing the reagents followed by the incubation for 2 h at 60 degrees C. The sensitivity and specificity of the technique were sufficient to detect 10 fg DNA in dot-hybridisation without cross-reaction with 10-fold excess of a heterologous DNA. This technique permits genome libraries screening.

Antibody Affinity↗
PubMed1993

[The primary interaction with DNA of "latent high-avidity antibodies" from gamma-globulin preparations, probably, is determined by the presence of a cofactor].

Highly positive by Farr assay, native DNA-binding fraction was derived from commercial normal human gammaglobulin preparations by ion-exchange chromatography. Human serum immunoglobulins of any isotype were not involved into this binding. The existence of nonimmunoglobulin factor primarily binding native DNA and intermediating DNA-Ig interaction is assumed.

Antibody Affinity↗
PubMed1993

[The replacement of accessory T-lymphocytes by synthetic peptides during the formation of splenic hematopoietic colonies].

Whether accessory T cells can be replaced by the synthetic immunomodulators thymogen (Glu-Trp) and thymohexin (Arg-Lys-Asp-Val-Tyr-Arg) was studied. The latter immunomodulator was found to show a 3-fold increase in splenic colony formation after incubation of bone marrow cells with rabbit antimouse brain serum (RAMBS). The former preparation failed to show the same action. Its effect was close to that of thymocytes. When the recipients exposed to lethal irradiation were administered the RAMBS-treated bone marrow cells and one of the peptides, it was shown that in concomitant administration, thymohexin and thymocytes lost their ability to restore colony formation by RAMBS-treated bone marrow. Thymogen did not suppress the stimulating activity of thymocytes. It is suggested that the differences observed between the tested peptides in their ability to recover colony formation were determined by their structure.

Adjuvants, Immunologic↗
PubMed1991

[Monoclonal antibodies to DNA, modified with osmium tetraoxide in the presence of 2,2'-bipyridine].

A monoclonal antibody (IgG) has been produced that binds to DNA modified with osmium tetroxide and 2,2'-bipyridine and does not react with unmodified DNA and modified or unmodified RNA and proteins. The reaction specificity is due to the presence of the modified deoxythymidine residue within the epitope. Possible use of the antibody for studies of DNA structure and detection of cDNA probes is discussed.

2,2'-Dipyridyl↗
PubMed1991

["Masked" autoantibodies from the serum of healthy blood donors cross-reacting with DNA and bacterial lipopolysaccharides].

The "masked" autoantibodies with polyspecific activity were found in the serum of healthy donors. It is shown that these antibodies are able to react with antigens after the ion exchange chromatography with QAE-sephadex. It was found that these "masked" antibodies are able to cross-react with DNA and lipopolysaccharides of wide-spread species of bacteria. The possible role of these antibodies as in protection of organism from the external antigens as in the origin of autoimmune diseases is discussed.

Autoantibodies↗
PubMed1990

[Polyanions inhibit the binding of autoantibodies with some cellular proteins].

Effect of polyanions on reaction of the Ig from sera of patients with systemic lupus erythematosus and scleroderma with cellular proteins was studied by immunoblotting. It has been shown that dextran sulfate, heparin, denatured DNA and poly I inhibited the binding of autoantibodies with some polypeptides. The molecular weight of these antigens was determined. The molecular mechanism of immunological reactions studied and it's role in pathogenesis of autoimmune diseases is discussed.

Autoantibodies↗