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Biomedical subjects

A M Ugolev

Publications and source records attributed to A M Ugolev.

At least 19 recordsLinked to original sources
PubMed1995

[Isolated enterocytes as an object of functional morphology].

Morphological examination of isolated enterocytes obtained-from the rat jejunum initial region by mechanical method after the previous treatment with ethylendiamintetracetic acid disodium salt included staining with hematoxylin, carmin and janus green. Histochemical reaction to alcaline phosphatase, succinate dehydrogenase, lactate dehydrogenase, ATP-ase and glycosaminoglycans were performed. Significant resemblance between the main cytological and cytochemical characteristics of the isolated enterocytes and those of the intestinal epithelium was demonstrated. Morphophysiological examination of isolated enterocytes, incubated in media, containing different substrates (glucose, maltose, glycine, trioleine) and under effect of oxygenated and not-oxygenated media demonstrated that mitochondria intracellular topography and succinate dehydrogenase activity significantly depend on their functional state.

Animals↗
PubMed1995

Structural and functional analysis of glucose absorption mechanisms in the rat small intestine in vivo.

The absorption of glucose (free, and released from membrane hydrolysis of maltose) and water in the isolated loop of the rat small intestine was studied in chronic experiments. Even at maximum glucose (75 mmol/l) or maltose (37.5 mmol/l) concentrations the rate of glucose transfer by solvent drag and by diffusion did not exceed 13% and 25%, respectively, of the total rate of glucose absorption. Electron microscopic and immunocytochemical analysis revealed a significant widening of intercellular gaps in the basal epithelium region and an increase of actin density in the vicinity of the tight junctions and between the apical root filaments in enterocytes after glucose or glycine load in chronic and acute experiments. However, very rarely (in 1% of all cases), and only in chronic experiments, structural changes in the tight junctions such as "blisters" and dilatations were also recognised. It is concluded that under normal physiological conditions the absorption of glucose (free, and released from maltose hydrolysis) mainly uses active transport across the apical membrane of the enterocytes.

Animals↗
PubMed1993

[Proteases of the large intestine].

The large intestine was found to reveal an obvious activity of various aminopeptidases and genuine dipeptidases in dogs and in humans. A dipeptidase was found specific by its independence of the C-terminal configuration of the peptide. This peptidase in an intrinsic membrane protein. Physiological significance of these enzymes is discussed.

Animals↗
PubMed1993

[An analysis of the structural characteristics of the tight junction of the enterocytes of the rat small intestine during nutrient absorption (immunoelectron microscopic research)].

To analyze structural changes of intercellular relationships of the enterocytes during glycine, glucose, and triolein absorption the structural and immunocytochemical methods of electron microscopy were used. The study was carried out on the proximal part of the rat small intestine in acute and chronic experiments. In the acute ones glucose or glycine solutions (both of 10 and 40 mM) or triolein emulsion (0.5%) were inserted into the isolated small intestinal segments for 20 min. In chronic experiments the isolated loop of the small intestine was perfused with glucose solution (40 mM). Then the corresponding pieces of the intestinal tissue were fixed for structural and immunocytochemical studies. Rarely (in 1% of all cases), and only in chronic experiments, structural changes in the tight junctions--"blisters" and dilatations--were found. At the same time the analysis of the spatial distribution of actin filaments showed that during glucose and glycine absorption the antiactin tracers were found not only within microvilli and on the "root" filaments but also in vicinity of the tight junction and between terminal filaments. The results obtained agree with the hypothesis about the possibility of paracellular transport of some nutrients induced by sodium-dependent transport of glucose and glycine.

Amino Acids↗
PubMed1992

[The effect of stress on the enzyme systems of the enterocyte microvilli in rat pups and the possible mechanism of the transmission of stress effects from dam to progeny].

In suckling rats whose mothers had had an immobilisation stress or been injected HC and T4 at the last day of their pregnancy, the sucrase induction decreased in the small intestine, if the HC was injected on the 10th day of life. The hormone injection to 1-day suckling rats did not affect the induction. These effects seem to be exerted not by the direct permeation of hormone from mother to litter but to be mediated rather by a special factor "stressin". The latter is transferred from mother to litter and decreases the sensitivity of intestinal enzymes to exogenous HC, thus exerting an antistressor effect.

Animals↗
PubMed1992

[The enzymatic barrier of the small intestine].

The small intestine's barrier functions are reviewed. The data on mechanical (passive) and active protective systems of the organism against various antigens, toxic substances and proteins, is presented. An important role of these protective systems as an enzyme apparatus of epithelial and postepithelial layers of the small intestine's mucose, is shown.

Animals↗
PubMed1992

[The detailed characteristics of the enzyme spectrum of the small intestine in rats in the early postnatal period].

The activity of the small intestine's peptide hydrolases is higher in 1-day old rats than in adult rats, whereas levels of activity of alkaline phosphatase and diglycyl glycine peptidase do not differ significantly in these two groups of the rats. Our own data on carbohydrases corroborate other authors' evidence and reveals that activities of lactase, sucrase and maltase are either absent or very low in the first days of life and sharply increase by the third week of postnatal development. Adaptive changes of regulatory properties of lactase and alkaline phosphatase are revealed.

Aging↗
PubMed1992

[The enzyme-transport characteristics of the small intestine of rats during aging].

Ageing was shown to be accompanied by changes of the activity levels of the small intestine's carbohydrases, dipeptidases, and alkaline phosphatases. The changes occurred both under normal conditions and after operations on the small intestine. In ageing, the small intestine's capacity for the homeomorphosis decreases irrespective of either increase or decrease in the functional loading. The age involution of the gastrointestinal tract is followed by disturbances not only in hydrolytic processes but in transport those as well: particularly deceleration of glucose accumulation in preparations of the rat intestine mucose. Regulatory properties of some enzymes were changed, too, in old rats.

Aging↗
PubMed1992

[Trans-sorption as an important mechanism of molecular transport in biological systems].

The mechanism of trans-sorption was shown to be based on mobile adsorption of various substances at specific and unspecific sites localised in spatially organised systems integrated within premembrane structures. Some specifics and possible physiological significance of trans-sorption are discussed using mathematical models of nutrients transfer across autonomous premembrane layer in the small intestine.

Animals↗
PubMed1992

[The transport system that pumps monosaccharides from enterocytes across the basolateral membrane].

Using a new, in vitro, experimental approach the transport of glucose, galactose, fructose and glycine in seroso-mucosal direction in the small intestine, was studied under different modes of oxygenation. In case of monosaccharides under the oxygenation from serosal surface, the substrate concentration is lower in the small intestine tissue than under anoxia. The effect of the oxygenation from serosal surface on the galactose transport was shown to be sensitive to absence of sodium ions, ouabain, phloretin, and not sensitive to phlorizin. The data obtained corroborate the hypothesis of the existence of a mechanism pumping off monosaccharides enterocytes through the basolateral membrane.

Animals↗
PubMed1992

[The characteristics of the absorption in the small intestine of 2 casein hydrolysates and of an equivalent amino acid mixture].

Using casein hydrolysates and the amino-acid mixture applied to mucosal or serosal surfaces, their effect on the transport of free glycine, glycine formed during glycyl glycine hydrolysis, and galactose, was studied in the small intestine. The data obtained suggest a mechanism of peptide transport in the enterocytes as well as a possibility of a regulatory effect of the peptides contained in casein hydrolysates on the transport of some basic nutrients.

Amino Acids↗
PubMed1992

[The digestive enzymes in the gastrointestinal tract, kidney, liver and spleen in different functional states].

A wide range of digestive enzymes with different cellular location (membrane, intracellular, Lysosomal) was determined in gastrointestinal organs (stomach, duodenum, jejunum, ileum, colon) as well as in undigestive organs (liver, kidney, spleen) in normal conditions and in altered functional states (fasting, refeeding). High levels of peptidase activity was noted in undigestive organs and the colon as compared to that in the small intestine. Adaptive responses were (revealed not only for a number of membrane enzymes but also for intracellular ones including those in undigestive organs.

Adaptation, Physiological↗
PubMed1992

[Theory of universal functional blocks and fundamental biomedical problems].

The paper presents the main aspects of the theory of elementary functional blocks. In accordance with the theory considered high specialization of the complex functions could be reached due to the recombination and transposition of a large though limited set of molecular machines realizing elementary biological operations. The authors proved that the theory of universal functional blocks became a fundamental aspect of the medical theory as it permitted one not only to explain and classify a certain part of clinical and clinicophysiological phenomenology but was of significant prognostic value.

Animals↗