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Biomedical subjects

A M Warren

Publications and source records attributed to A M Warren.

10 recordsLinked to original sources
PubMed1997

Zebedee: a novel copia-Ty1 family of transposable elements in the genome of the medically important mosquito Aedes aegypti.

We have utilised PCR to directly identify a novel family of copia-Ty1 retrotransposable elements (RTPs) in the genome of the mosquito Aedes aegypti. Two members of the family have been sequenced in their entirety and their structural characteristics determined. ZebedeeI is 3505 bp long and appears to be flanked by 21bp direct repeat sequences. A single open reading frame (ORF) of 972 amino acids has the coding potential for a polyprotein with homology corresponding to the conserved amino acid motifs of Long Terminal Repeat (LTR) retrotransposon protease, integrase and reverse transcriptase. ZebedeeII likewise shares significant homology with these regions and also appears to be flanked by short direct terminal repeat sequences of 22 bp. Fifty copies of the 22 bp repeat sequence are present abutting the 5' end of ZebedeeII, with two (partial) representatives of this repeat sequence being present at the 3' end. The Zebedee family appears to have a low middle repetitive copy number in different strains of Ae. aegypti; and transcripts of the elements have been detected in cultured mosquito cells by RT-PCR. Despite the lack of a gag homologue or the LTR hallmarks of previously characterised copia-Ty1 RTPs, phylogenetic analyses place Zebedee within this group, showing considerable homology to copia from Drosophila melanogaster.

Aedes↗
PubMed1994

Mariner: its prospects as a DNA vector for the genetic manipulation of medically important insects.

Originally described in Drosophila mauritiana, the mariner transposable element has very recently been identified in 63 other insect species, representing ten insect orders, and including the malaria-vector mosquito, Anopheles gambiae. Ann Warren and Julian Crampton here discuss how transposable elements can be exploited as valuable research tools for the molecular characterization of genomes and as DNA vectors for genome manipulation and the 'creation' of transgenic organisms.

Journal Article↗
PubMed1994

Adolescents and colposcopy: the use of different procedures to reduce anxiety.

OBJECTIVE: Two consecutively occurring studies examined whether using a video colposcope to view this procedure or allowing the female adolescent to watch music videos would reduce anxiety and related body movements. STUDY DESIGN: Female adolescents who underwent colposcopy were randomly assigned to one of two groups, experimental and control. In study 1, 27 female adolescents were randomly assigned either to view the procedure on a television monitor or to be part of a control group (no visual distraction). In study 2, 30 female adolescents were randomly assigned either to a music video group or to a control group. Studies were completed in a consecutive manner and used the same measures, colposcopic equipment, and professional staff, including physician. Multiple measures of anxiety were used (heart rate, behavior observation, and paper and pencil) before, during, and after the procedure. During each colposcopy the subject's behavior across 10 dimensions was observed and coded. Data were analyzed by chi 2, analysis of variance, and Student t tests. RESULTS: Study 1 found no significant differences in body movements and anxiety ratings between the video colposcope group and controls. In study 2, subjects who were allowed to watch the music videos demonstrated significantly fewer body movements indicative of pain, required less physician reassurance, and received fewer procedural explanations (p < 0.05). CONCLUSION: Results suggest that allowing a female adolescent to watch music videos during a colposcopic examination appears to decrease body movements associated with discomfort.

Adolescent↗
PubMed1991

The Aedes aegypti genome: complexity and organization.

We describe the use of DNA reassociation kinetics to determine the total genome size and complexity together with the individual complexity and copy number of the single copy, middle repetitive and highly repeated DNA fractions of cell line and larval DNA from the mosquito, Aedes aegypti. The genome of Ae. aegypti is both large and complex, being one third the size of the human genome, and exhibits a short period interspersed repeat pattern. The implications of patterns of sequence arrangement and genome complexities for experiments aimed at isolating specific classes of DNA sequences, such as mobile genetic elements, are discussed.

Aedes↗
PubMedDate not supplied

Altered family processes related to an ill family member: a validation study.

The purpose of this retrospective descriptive survey was to validate the defining characteristics for altered family processes related to an ill family nursing diagnosis. Using Ferhing's (1987) Diagnostic Cantent Validation Model, data were collected from 58 registered nurses. Clinical nurses submitted 14 defining characteristics not identified by NANDA nor found in the literature. Many of these characteristics expressed the same concepts in different terms from those used by NANDA. The results of this study give credibility to NANDA's conceptually stated defining characteristics and suggest further clinical research.

Adaptation, Psychological↗
PubMedDate not supplied

Pinch grafts--applications in podiatric wound closure.

Pinch grafts were initially described in the late nineteenth century as a technique that could be utilized, under selected conditions, to promote wound healing. This procedure remains a viable component of the podiatric surgical armamentarium. There has been relatively little information on this subject in the literature: podiatric, orthopedic, or general medical, during the past several decades. A review of the historic development of the procedure, and case presentations, for examples of current applications, are presented.

Aged↗
PubMedDate not supplied

Verrucous squamous cell carcinoma.

Verrucous squamous cell carcinoma involving the plantar aspect of the foot is a relatively rare and generally slow growing tumor that is capable of great local destruction. Diagnosis is based on history, clinical appearance and, most importantly, deep biopsy. A case report is presented, along with a literature review of the history, treatment and prognosis of this rare entity.

Biopsy↗
PubMedDate not supplied

Current therapy for subungual melanoma.

Malignant subungual melanoma is an infrequently encountered but often misdiagnosed clinical entity. The podiatric surgeon plays a major role in the early diagnosis and surgical management. An illustrative case report of its clinical presentation and a thorough review of current therapeutic modalities are presented.

Aged↗
PubMedDate not supplied

Fibrolipomatous hamartoma of nerve.

Fibrolipomatous hamartomas of nerve are rare, benign, fibrofatty malformations of peripheral nerves, most commonly affecting the median nerve. Lower extremity cases are extremely rare. The authors present a very rare case of a fibrolipomatous hamartoma involving the superficial peroneal nerve, and review the literature regarding its clinical presentation and surgical management.

Adult↗