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Biomedical subjects

A Makino

Publications and source records attributed to A Makino.

10 recordsLinked to original sources
PubMed1992

The peripheral nerve allograft in the primate immunosuppressed with Cyclosporin A: I. Histologic and electrophysiologic assessment.

Nerve regeneration across peripheral nerve allografts and control autografts in primates immunosuppressed with Cyclosporin A was quantitatively evaluated by electrophysiologic and histologic methods. Twelve cynomolgus monkeys received 3-cm autografts and allografts in contralateral ulnar nerves. They were immunosuppressed with Cyclosporin A at 25 mg/kg per day or placebo vehicle. Morphometric analysis of nerve graft and distal nerve segments was assessed at 1 year after engraftment. Quantitative electrophysiologic studies were performed percutaneously at 6 and 12 months, and compound action potentials were measured directly across the nerve grafts at 1 year. Excellent regeneration was seen across autografts and allografts in Cyclosporin A-treated and placebo-treated recipients.

Action Potentials↗
PubMed1991

Coexistence of intracranial and spinal meningiomas--report of two cases.

The authors report two rare cases of multiple meningiomas in both the intracranial and spinal regions. A 64-year-old female presented with a right sphenoidal ridge meningioma and a cervical extramedullary meningioma. Tumor histology was transitional and vacuolated types, respectively. The tumors were removed successfully in two stages, craniotomy then laminectomy 3 months later. A 62-year-old female presented with a right sphenoidal ridge meningioma (meningotheliomatous type) which was totally removed. An extramedullary spinal meningioma became symptomatic 33 months later, which was also removed totally. The meningiomas in the first case had different subtypes, but immunohistochemical characteristics including microcyst formation were similar. The second case had meningiomas of the same subtype with similar characteristics, but different fibrous septum development. Multiple meningiomas, even in different compartments of the central nervous system, may have common characteristics.

Biomarkers, Tumor↗
PubMed1990

Analysis of the oligosaccharides in ovalbumin by high-performance capillary electrophoresis.

The oligosaccharides in ovalbumin as a glycoprotein model were released with anhydrous hydrazine, and reductively pyridylaminated after re-N-acetylation. The derivatives were analyzed by capillary zone electrophoresis (CZE) with on-column fluorometric detection. Direct CZE could separate the derivatives on the basis of the degree of polymerization, giving five peaks of hepta-, octa-, nona-, deca-, and undecasaccharides. Coelectrophoresis with the standard mixture of isomaltooligosaccharide derivatives was effective for peak assignment. CZE as borate complexes allowed separation on the basis of structural difference, especially in the peripheral monosaccharide residues. Peaks were tentatively assigned to the derivatives of reported oligosaccharides by comparing their relative mobilities with those of the chromatographic fractions obtained by using the ODS and Dowex 50W x 2 columns. These two modes gave excellent separation and were complementary to each other. Although the actual amount analyzed in the capillary tube was quite small (ca. 5 ng as carbohydrates), a larger amount (ca. 25 micrograms as carbohydrates) was required to make sample concentration sufficiently high to be detected by a modification of a commercial fluoromonitor for HPLC.

Animals↗
PubMed1989

Simultaneous determination of reducing monosaccharides by capillary zone electrophoresis as the borate complexes of N-2-pyridylglycamines.

Reducing monosaccharides were derivatized to N-2-pyridylglycamines and separated as their borate complexes by capillary zone electrophoresis, using a capillary tube (50 microns i.d., 65 cm) of fused silica containing 200 mM borate buffer (pH 10.5) as carrier. The derivatives of 12 saccharides were completely separated in ca. 25 min, with high resolution, at an applied potential of 15 kV. On-column uv monitoring allowed detection of these derivatives at the 10-pmol level, and quantification by the relative peak area method allowed reproducible determination of these saccharides at least in the concentration range of 10-100 mM in reaction solutions. This method was applied to the determination of the monosaccharide compositions of various carbohydrate materials to demonstrate its usefulness.

Aminopyridines↗
PubMed1988

Cortical blindness caused by acute general cerebral swelling.

A 7-year-old girl who suffered from acute general cerebral swelling as a result of a traffic accident showed cortical blindness. Computed tomography (CT) scan on admission revealed marked slitlike ventricles and narrowing of the perimesencephalic cisterns, which indicated general cerebral swelling. While hospitalized, the patient developed transtentorial herniation twice on day 3, and CT scans at herniation episodes showed disappearance of the perimesencephalic cisterns. After recovery of consciousness, the patient showed cortical blindness, and during gradual recovery she showed pure alexia without agraphia. The visual evoked potentials at 8 weeks, 16 weeks, and 3 years 4 months after trauma showed normalization of the pattern, but revealed left occipital inactivity.

Acute Disease↗
PubMed1988

[Normal skin cells in vitro].

Major cells in skin are epidermal cells in epidermis and fibroblasts in dermis. These cells can be isolated as a relatively pure population from the tissues using proteases and chelating agents. In this review we describe the way of culture where these two kinds of cells express normal function as they do in vivo. 1) It is important to consider the polarity of epidermal cell membranes in the transport of nutrients and metabolites when the cells are to be cultured in a healthy state in a long period. Epidermals cells expressed their normal polarities when cultured on a porous thin film of collagen and bathed on both sides (apical and basal) in culture media. 2) It is important to consider the interactions of fibroblasts with collagen when normal morphology and physiology are expected to be expressed in the cell in culture. Collagen affected the morphology of the cell and profoundly decreased the rate of DNA synthesis. We present a hypothesis which explains the fibronectin-independent interaction of fibroblasts with collagen. 3) It is important to consider the interactions between fibroblasts and epidermal cells when normal physiology of the skin as a whole is expected to be expressed in vitro, because exchange of information between them control their metabolic activities and functions. In this review, two examples for this exchange are presented: cell growth and collagenolysis.

Cell Communication↗
PubMed1987

Tissue-typing in human massive allografts of frozen bone.

The cases of twenty-six patients who received a massive allotransplant of frozen bone, with a known degree of histocompatibility between the donor and the recipient, were studied. Twenty-two patients were followed for more than two years (range, twenty-four to ninety-two months). Twenty-three biopsies were performed in sixteen patients from nine to seventy-eight months after transplantation. No clear relationship could be established between the degree of histocompatibility of the donor and the recipient and the incorporation of the graft, probably in part due to the number of variables involved and the polymorphism of the HLA system. However, no early massive resorption of the transplant was seen in this series, in which, by the design of the protocol, no recipients had pre-existing circulating antibodies to the antigens of the donor. Two allografts showed infiltration by round cells and vascular lesions in the absence of infection, which is suggestive of an immune response against antigens from the donor. Both matched poorly with the donor for HLA antigens. The individual who had the strongest reaction was the only recipient in the series who had a massive failure of the transplant.

Bone Transplantation↗
PubMedDate not supplied

Bilateral occlusions in the cervical portion of the internal carotid arteries in a child.

An 8-year-old boy with a history of recurrent tonsillitis and cervical lymphadenopathy developed acute hemiplegia. Cerebral angiography revealed bilateral occlusions of the cervical portion of the internal carotid arteries. Cerebral arterial occlusion in childhood has been attributed to many causes, e.g., trauma and infections of the neck. In the present case, though not clearly proved, inflammatory processes may have been related to the occlusion.

Arterial Occlusive Diseases↗