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Biomedical subjects

A Martí

Publications and source records attributed to A Martí.

At least 19 recordsLinked to original sources
PubMed2003

Influence of pulsation rate on udder health and teat thickness changes in dairy ewes.

In this work on machine milking of ewes, pulsation rates of 120 and 180 cycles per min were compared, both with a pulsation ratio of 50:50 and a vacuum level of 36 kPa, comparing intramammary infection (IMI), somatic cell count (SCC) and teat end thickness changes. To this end, two groups of 20 Manchega ewes were used in a crossover experimental design with two experimental periods of 24 d for each. Bacterial exposure of all teats was increased by dipping them in a suspension of Staphylococcus simulans at four consecutive milkings of each period. Pulsation rate of 180 cycles per min, compared with 120 cycles per min, had no negative effect upon new IMI (11 and 16% of ewes infected, respectively) and SCC. No teat end lesions were observed in those animals milked with the two pulsations assayed. Also, teat thickness changes (-0.38 and -0.36 mm at 120 and 180 cycles per min, respectively) were not affected significantly. Finally, in absence of IMI, the two pulsation rates assayed did not affect the SCC.

Animals↗
PubMed2003

Effects of once versus twice daily milking throughout lactation on milk yield and milk composition in dairy goats.

The effects of once (1X) vs. twice (2X) daily milking throughout lactation on milk yield, milk composition, somatic cell count (SCC), and udder health were studied in 32 Murciano-Granadina dairy goats. Goats were assigned at wk 2 of lactation to two treatment groups; once daily milking at 0900 (1X, n = 17), or twice daily milking at 0900 and 1700 (2X, n = 15). Milk yield was recorded weekly until wk 28, and milk composition and SCC were evaluated for each individual udder half at each milking at wk 2 and 4 of lactation and then, monthly until the end of the experiment. Once daily milking resulted in an 18% reduction in the yield of 4% fat-corrected milk compared to twice daily milking (1.61 vs. 1.95 L/d, respectively). This reduction was more marked from wk 2 to 12 than in mid and late lactation. Response to milking frequency also varied according to parity number where goats of less than four parities suffered more milk yield losses during 1X than older goats. Milk of 1X goats contained higher percentages of total solids (13.6 vs. 12.9%), fat (5.10 vs. 4.62%) and casein (2.57 vs. 2.35%) than milk of 2X goats, but milk protein percentage did not differ between treatments (3.28 vs. 3.20%). Yields of total solids, fat, protein and casein tended to be higher for 2X than 1X. Milk SCC did not differ between treatments. We conclude that application of once daily milking in Murciano-Granadina dairy goats moderately reduced milk yield without negative effects on milk composition and udder health. Losses in milk yield would be reduced if 1X is practiced during mid- or late lactation and in older goats. An increase in labor productivity and a higher farmer's standard of living is also expected.

Animals↗
PubMed2002

A maximal effort trial in obese women carrying the beta2-adrenoceptor Gln27Glu polymorphism.

The aim of this study was to observe the relationship of the beta2-adrenoceptor (ADRB2) gene polymorphism Gln27Glu in a group of obese female subjects submitted to an acute physical activity test. Six Glu27/Glu27 obese women were compared with six Gln27/Gln27 obese women (coupled by age, BMI, waist circumference, percentage of fat mass and absence of smoking). These groups were selected after having genotyped 91 obese subjects (BMI>30 kg/m2, age 20-60). Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used to genotype the obese population. The physical activity challenge consisted of a Maximal effort trial (VO2 max) following the Bruce protocol on a walking treadmill. The frequency for the Glu allele was 0.42, which is similar to other Caucasian populations. In basal conditions, subjects with the Glu27/Glu27 genotype showed a higher value of plasma insulin, while circulating glucose levels were similar in both groups. Also, diastolic arterial pressure was higher in the Glu27/Glu27 obese women. After the VO2 max trial, the Glu27 group had a significantly higher respiratory exchange ratio than the Gln27 group suggesting a lower post- exercise fat oxidation. These results provide evidence about a possible impact of the Gln27Glu polymorphism in the ADRB2 gene concerning a better response to exercise in obese Gln27 female subjects, in which basal insulinemia may play a role.

Blood Glucose↗
PubMed2002

[Causes of obesity].

The aetiology and treatment of obesity requires a knowledge of the mechanisms that control the homeostasis of the fuel substrates and adiposity. The processes of regulation adjust the supply of macronutrients and energy demands with the aim of maintaining a stable body mass. In the light of the most recent research, the hypothesis can be advanced that the control of body weight and its composition depends on an axis integrated by three self-regulated components: appetite, stores thermogenesis and fat deposits. The most important factors involved in obesity seem to be dietary habits diet and physical activity, which are affected by genes, which in their turn affect energy expenditure, the metabolism of energy substrates and food consumption. However, the growing rates of obesity cannot be explained exclusively by genetic causes, since they are in some cases associated with the consumption of diets with a high energy density or rich in fat, and by a growing sedentary life style in society, both in developed and developing countries. The study of genetics and life style involved in the increase of body weight and obesity can facilitate the implementation of preventive actions.

English Abstract↗
PubMed2002

[Methodology for the study of the regulation of body weight and/or the aetiology of obesity].

Obesity, one of the most serious health problems of developed societies, is the result of a positive energy balance maintained over time. The regulation of body weight and its maintenance seem to be regulated by an axis of three interdependent components: dietary intake, energy expenditure and adipogenesis, although it is necessary a deeper understanding of the processes involved in the oxidation of nutrients and in the energy balance. The metabolic processes are numerous and inter-related, it is however convenient to study them separately in such a way that it is possible to obtain a better knowledge of the complete system. Indeed, this is the procedure employed in the design of numerous experiments, be they on laboratory animals, cellular cultures, or even on human beings when the molecular mechanisms involved in the regulation of body weight are examined. Both in vitro methods (cellular and subcellular models) and in vivo ones have made it possible to increase knowledge of the mechanisms that control body weight. It is worth underlining the new strategies that the molecular era and provides us with, although the techniques that consider the human organism as a whole might have a notable influence on the development of new drugs, together with therapeutic management and the prevention of obesity.

English Abstract↗
PubMed2001

Obesity and immune function relationships.

The immunological processes involved in the collaborative defence of organisms are affected by nutritional status. Thus, a positive chronic imbalance between energy intake and expenditure leads to situations of obesity, which may influence unspecific and specific immune responses mediated by humoral and cell mediated mechanisms. Furthermore, several lines of evidence have supported a link between adipose tissue and immunocompetent cells. This interaction is illustrated in obesity, where excess adiposity and impaired immune function have been described in both humans and genetically obese rodents. However, limited and often controversial information exist comparing immunity in obese and non-obese subjects as well as about the cellular and molecular mechanisms implicated. In general terms, clinical and epidemiological data support the evidence that the incidence and severity of specific types of infectious illnesses are higher in obese persons as compared to lean individuals together with the occurrence of poor antibody responses to antigens in overweight subjects. Leptin might play a key role in linking nutritional status with T-cell function. The complexities and heterogeneity of the host defences concerning the immune response in different nutritional circumstances affecting the energy balance require an integral study of the immunocompetent cells, their subsets and products as well as specific and unspecific inducer/regulator systems. In this context, more research is needed to clarify the clinical implications of the alterations induced by obesity on the immune function.

Adipose Tissue↗
PubMed2001

Association between prematurity and maternal anemia in Venezuelan pregnant women during third trimester at labor.

To determine the association and its magnitude between prematurity and anemia in women in their third trimester of pregnancy and at labor. An incident case-control study was conducted using 2 controls per case. Data was obtained in a tertiary hospital in Valencia, Venezuela. A total of 543 women who delivered between May and December 1996 entered into the study. Women having a preterm delivery, less than 37 weeks of gestation at delivery, were defined as cases (n = 181). Anemia was defined according to WHO as Hb less than 11 g/dL. Logistic regression was used to analyze the data and likelihood ratio test was done for model comparison. Maternal anemia was found to be significantly associated with prematurity (Odds Ratio: 1.70; 95% CI = 1.18 to 2.57 P = .001), after adjusting for Placental Abruption, PROM, Previous Premature Labor, Prenatal Care Visits, and Uterine Bleeding during more than one trimester. Maternal anemia at the end of the third trimester of pregnancy, at labor, was associated with an increased risk of prematurity.

Anemia↗
PubMed1999

Leptin: physiological actions.

Leptin, a peptide hormone (167 aa) mainly expressed in adipocytes, and its hypothalamic receptors are integral components of a complex physiological system evolved to regulate fuel stores and energy expenditure. Thus, leptin discovery has constituted a great breakthrough in the understanding of body weight regulation and in the role of the fat tissue as an endocrine organ. Increasing scientific evidences suggest that, leptin has overall effects on metabolism. Leptin mRNA and/or protein are produced by placenta, fetal tissues, gastric mucosa and hepatic stellate cells and can participate in many physiological functions such as fetal growth, gut-derived satiety, immune or proinflammatory responses, reproduction, nutrient intestinal absorption, angiogenesis and lipolysis. The leptin participation in body weight homeostasis and obesity as well as other peripheral actions are revisited.

Animals↗
PubMed1997

[Shortened version of the Barcelona test (II): global standard score].

The aim of this study was to determine a global scoring method for a shortened and version of the Barcelona Neuropsychological test (TB) of 55 items. 341 healthy volunteers, 178 male (52.19%), and 163 female (47.1%) were studied. Age mean: 54.80, SD = 17.44. Subjects were stratified in five groups considering age and education. A scale of cut-off points was selected in order to divide the scores of each subtest into three bands (categories): 0 (lowest), 1 (intermediate), 2 (highest). Raw scores (possible range 0-110) were obtained and then were converted to a mean of 100 and SD of 15 each normative group. Tables of normative data were obtained for each group. This study shows the cognitive profiles of the TB and a global standard score related to them. This global score will provide new possibilities in the neuropsychological assessment of neurological patients.

Aged↗
PubMed1997

[Shortened version of the Barcelona test (III): criterion validity with the ADAS-Cog].

The aim of this study was to assess the criterion validity of a global score of an abbreviated version of the Barcelona Test (BTA), normalized for age and education, as a clinical neuropsychometric test for the assessment of cognitive impairment. This study made a correlational analysis between test scores of the BTA and scores of the cognitive part of the Alzheimer's Disease Assessment Scale (ADAS-Cog) adjusted for age and education. It was made in a University Hospital (Dementia Unit). A group of 172 subjects (74 male, 98 female) was distributed according the Global Deterioration Stages (GDS) from 1 to 6. GDS-1 (normal controls), n = 60; GDS-2 (subjective complaints), n = 27; GDS-3, n = 21, GDS-4, n = 22; GDS-5, n = 16; GDS 6, n = 26. All dementia patients fulfilled the NINCDS/ADRDA criteria. BTA global scores correlated significantly with adjusted scores on the ADAS-Cog (r = 0.872, r2 = 0.761), p < 0.0001. The BTA has a criterion validity as a clinical neuropsychometric test for the study of cognitive impairment.

Adult↗
PubMed1995

Subtyping of Legionella pneumophila isolates by arbitrarily primed polymerase chain reaction.

Arbitrarily primed polymerase chain reaction (AP-PCR) was used to differentiate strains of Legionella pneumophila isolated from different water sources in a resort hotel in Benidorm, Alicante, Spain, where an outbreak of Legionnaires' disease occurred among a group of tourists between 65 and 80 years of age. All isolates were L. pneumophila serogroup 1, subtype Pontiac (Knoxville 1). Five different patterns (P1 to P5) were obtained by AP-PCR. The number of bands per pattern varied between 4 and 11. Patterns P1 and P2 represented 60 and 20% of L. pneumophila isolates, respectively. Since different subpopulations of L. pneumophila coexisted (up to three different AP-PCR patterns were identified in a single room), it was not possible to link an individual L. pneumophila strain to the occurrence of this outbreak.

Antibodies, Monoclonal↗
PubMed1994

Serum IgA anti-Klebsiella antibodies in ankylosing spondylitis patients from Catalonia.

IgA antibodies against Klebsiella pneumoniae were measured by immunofluorescence in 84 Catalan patients with ankylosing spondylitis (AS), 41 patients with non-inflammatory arthropathies (NIA) and 22 patients with rheumatoid arthritis (RA). Patients with AS showed higher levels of anti-klebsiella IgA antibodies (IgA-Kp) than NIA and RA patients (4.7 +/- 1.6 U vs 3.7 +/- 1.5 U and 3.1 +/- 1.4 U respectively, p = 0.001). In AS patients a significant correlation between IgA-Kp and levels of C-reactive protein was observed. Although no clear correlation was found between IgA anti-klebsiella and total serum IgA levels, a significant correlation between IgA anti-klebsiella and serum levels of secretory IgA was detected (r: 0.43, p = 0.003). In conclusion, some patients with AS disclosed raised levels of Klebsiella antibodies in sera and this is related to an increase of secretory IgA level. Analysis about the relationship between response to klebsiella and the presence of gut inflammation in AS patients could be of interest.

Adolescent↗
PubMed1994

Stimulation of brush border enzyme activity along the rat small intestine by misoprostol.

The effect of Misoprostol (0.3 mg/kg b.w., orally for four weeks) on the brush border membrane enzyme activity, is studied in growing rats. Misoprostol enhanced stomach and intestine relative weights as well as the mucosal weight of the duodenum and proximal jejunum. In treated rats, disaccharidases, alkaline phosphatase and aminopeptidase enzyme activity were measured in brush border purified fraction throughout the small intestine. Sucrase, maltase, aminopeptidase and alkaline phosphatase specific activities were significantly increased along the small intestine. In the proximal jejunum, sucrase (62%; p < 0.001) and maltase (42%; p < 0.01) activities were significantly greater. Sucrase activity was also significantly (p < 0.001) increased by about 103% in the distal jejunum. There was also a significant (p < 0.05) increment of 32% in the duodenal and ileal alkaline phosphatase activity after treatment. Similarly, aminopeptidase activity was significantly (p < 0.05) higher in duodenum (67%) and jejunum (24%). In conclusion, Misoprostol appreciably increased the ability of the small intestine to perform its digestive functions although further studies will be necessary to examine the cellular and molecular mechanism(s) which may be responsible for these effects.

Alkaline Phosphatase↗
PubMed1993

Controlled release of theophylline from inert matrices: effect of channeling material on release rate.

Sustained release theophylline tablets containing stearic acid wax and lactose fast flo as chanelling agent were prepared and evaluated. The fusion technique was used for dispersing the drug in the different levels of stearic acid. The release rate of theophylline from the prepared tablets increased with the increase of the level of the channeling material in the formula. The drug release from tablets containing high level of wax (30 and 60%) and low level of channeling material (59% and 29%) followed the diffusion controlled model for inert porous matrix. The drug release increased significantly with the increase of lactose fast flow level in the formula. After 2 hours of testing dissolution, the tablets start to erode and the mechanism of drug release deviate from the diffusion controlled model. The mechanism of drug release was dependent on the level of the channeling material in the matrix.

Chemistry, Pharmaceutical↗
PubMed1991

[Physician-patient communication and health education].

The aim of the present study was to analyze the content in health education of the patient-physician interview. The study was observational and descriptive. The target population were the patients attending the general practices of the Social Security clinics. With permission of patients and physicians, 53 visits of two general practitioners were recorded on tape. 48 interviews were analyzed with definition of activities (asking, answering, discussing, etc.) and contents (health problems, treatment, health education, etc.). Health education was 10% of the contents, and it was associated with the physician's activity of commenting/instructing and with the patient's activity of commenting/asking.

Interviews as Topic↗
PubMed1988

Immunohistochemical study of bone marrow sections in CLL.

T lymphocytes and dendritic reticulum cells (DRC) were studied in frozen-cut bone marrow sections of 35 patients with chronic lymphocytic leukemia (CLL) (infiltration patterns: interstitial 8, nodular 6, mixed 9, diffuse 12) and 13 cases of low grade non Hodgkin's lymphoma (NHL) (centroblastic/centrocytic 7, centrocytic 3, lymphoplasmacytoid 3) with bone marrow involvement. In contrast to the usual findings in normal bone marrow, in CLL and low grade NHL CD4 positive cells were more numerous than CD8 positive cells. Whereas in NHL CDR were large and occupied all the nodule, in CLL were small and located in the center of the nodule. These findings can be of interest in the study and differential diagnosis of lymphoproliferative disorders.

Antigens, Differentiation, T-Lymphocyte↗
PubMed1985

The influence of a new compound, PATP, on glucose transport in the isolated fat cell.

A newly synthesized compound, N-(4-pyridylcarbonyl amino) 1, 2, 3, 6 tetrahydropyridine (PATP) was earlier found to elevate the blood glucose level in rats. This suggested that the compound might be accomplishing this by blocking glucose transport into tissue cells. This hypothesis has now been examined in the isolated fat cell system using a modification of the method to improve the accuracy of measurements made. This study indicated that PATP is a competitive inhibitor of glucose transport and metabolism (KI = 0.89 mM) but could not unequivocally prove that the effect was on transporter action alone. That the compounds action was at this level, however was shown by its ability to inhibit the uptake of the transported but non-metabolized sugar, 3-0-methyl glucose (KI = 3 mM). PATP is a nonphenolic inhibitor of glucose transport unrelated in structure to the sugar or to another more potent inhibitor, phloretin.

Adipose Tissue↗