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Biomedical subjects

A Masunaga

Publications and source records attributed to A Masunaga.

9 recordsLinked to original sources
PubMed1992

Primer-dependent amplification of mdr1 mRNA by polymerase chain reaction.

We have developed a sensitive reverse transcription (RT)-polymerase chain reaction (PCR) to evaluate multidrug resistance in various tissues, using seven mdr-specific primer sets. mdr1 mRNA expression was noted in 16/197 samples. However, the apparent degree of mdr1 mRNA expression depended on the sequence of the primers employed. These findings suggest that more than two sets of primers should be used for effective RT-PCR.

Base Sequence↗
PubMed1992

A case of intramural uterine stromal tumor with epithelial differentiation.

A case of intrauterine tumor in a 62-year-old Japanese woman is presented. It was thought initially that this was a case of uterine tumor resembling an ovarian sex cord tumor. To examine the cytological features of the tumor cells, electron microscopical and immunohistochemical studies were done, and a hormone assay of the tumor tissue was performed. The tumor cells were rich in rough endoplasmic reticulum (rER), mitochondria and microfilaments. Some tumor cells tended to form glandular patterns, but these epithelial elements were frequently scattered among fibrous stromal elements. Though many tumor cells with an epithelial appearance possessed a large quantity of cytokeratin and vimentin, they did not secrete estradiol, progesterone, testosterone or human chorionic gonadotropin. This case was finally diagnosed as an intramural uterine stromal tumor with epithelial differentiation after taking all the available data into consideration. This would be classified as an endometrial stromal tumor with epithelial elements, recently proposed and named by Clement and Scully.

Antigens, Neoplasm↗
PubMed1991

Reduced tenascin expression in colonic carcinoma with lymphogenous metastasis.

We have studied expression of tenascin (TN) in colonic carcinoma cells from 81 patients with colonic carcinoma without (20) and with (61) lymphogenous metastases, in order to assess whether TN plays a role in local invasiveness and metastasis of tumors. In metastatic colonic carcinoma tissues from 52 cases, moderate, partial expression of TN was observed in the primary foci but no TN expression was observed in tissues from 9 others. However, in every case of nonmetastatic colonic carcinoma, very strong TN expression was observed in the tissues. Furthermore, the presence of dense TN accumulation correlated well with the prognoses (1-year survival) of colonic cancer patients (p less than 0.01). Weak TN expression was observed in 24/61 of the metastatic lymph nodes examined. In 2 patients with metastatic colonic carcinoma, the colonic cancer cells produced TN. TN may, therefore, play a role in limiting or preventing local tumor invasion rather than preventing metastasis and is a useful marker for predicting the prognoses of patients with colonic cancer.

Adult↗
PubMed1990

Expression of T-cell receptor (TCR)alpha chain on normal human tonsils and T-cell lymphomas.

We analyzed immunohistologically the expression of T-cell receptor (TCR)alpha chain on human tonsils and on T-cell lymphoma (T-ML) tissues using the avidin-biotin-peroxidase method. A murine monoclonal antibody alpha F1, specific for the constant region of the TCR alpha chain, was employed. On normal tonsil, alpha F1-positive cells were observed mainly in T-zones and germinal centers. In T-zones, the staining intensities varied markedly, with heavy staining evident in less than one fourth. In germinal centers, a proportion of stained cells showed a histiocytic pattern with small cytoplasmic projections. All the T-ML tissues expressed TCR alpha, whereas the staining intensities varied among cases and among lymphoma cells. No correlations were observed in the expressions of TCR alpha chain and other T-cell markers including CD3, CD4 and CD8.

Antigens, Differentiation, T-Lymphocyte↗
PubMed1990

[Fifty two-week chronic oral toxicity study of mofezolac (N-22) in beagle dogs].

The chronic toxicity study of mofezolac (N-22), a new developed analgesic and anti-inflammatory agent, was carried out in beagle dogs by oral administration of the test compound at dose levels of 1, 3, 6 and 12 mg/kg once daily for 52 weeks. The recovery study was also carried out by withdrawal for 5 weeks. 1. No death occurred during the administration and recovery periods in all groups, there were no abnormal findings due to N-22 administration on behavior and appearance, body weight and food consumption. 2. There were no abnormal findings due to N-22 administration on fecal test, urinalyses, hematological and biochemical examinations, hepatic and renal function tests, ophthalmological examination, electrocardiography, blood pressure and organ weight. 3. By histopathological examination, loss of villi in small intestine was found in one female of 6 mg/kg group, erosion in small intestine in one male of 12 mg/kg group, and shortening of villi and ulcer in small intestine in each one female of 12 mg/kg 4. Accordingly, the non-effective dose levels of N-22 were estimated to be 6 mg/kg for male and 3 mg/kg for female in this study.

Administration, Oral↗