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Biomedical subjects

A Mattheus

Publications and source records attributed to A Mattheus.

At least 19 recordsLinked to original sources
PubMed1997

Use of XTT-assay to assess the cytotoxicity of different surfactants and metal salts in human keratinocytes (HaCaT). A feasible method for in vitro testing of skin irritants.

Because of the increasing need of reliable skin irritation tests and in order to reduce the number of animal experiments, in vitro alternatives have to be developed. We studied four surfactants and five metal salts for their cytotoxic potency in HaCaT cells, a spontaneously immortalized human keratinocyte line. The endpoint used to assess cellular viability was metabolization of the tetrazolium salt XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide). The tested substances revealed a significant rank order of their cytotoxicity at an exposure time of 24 h. It was 1) benzalkonium chloride, 2) sodium lauryl sulphate, and 3) Tween 20 (polyoxyethylene sorbitanmonolaurate) and Tween 80 (polyoxyethylene sorbitanmonooleate), for the surfactants; and 1) potassium bichromate, 2) copper sulphate, 3) cobalt chloride and palladium chloride, and 4) nickel sulphate, for the metal salts. There is an excellent correlation to the rank order of their known irritative potency in vivo. Being practicable and effective, the presented XTT-assay on HaCaT cells would be well suitable for an initial orientating screening of substances, subsequently followed by irritation tests directly in humans.

Benzalkonium Compounds↗
PubMed1989

[Studies on the detection of antibodies to Neisseria gonorrheae using pilus antigen].

The formation of antibodies against pilus antigen of strain F-62 was studied by indirect hemagglutination in the sera of 100 patients. The criterion for the diagnosis of gonorrheal disease was a positive culture of Neisseria gonorrhoeae. A positive smear test (staining with methylene blue) was accepted only in a few cases if the chain of infection could be followed completely. Retrospective studies showed that 75% of our patients with gonorrheal disease had antibodies against pilus antigen. The specificity was 91.7%. Attention is drawn to problems involved in evaluating the test results.

Antibodies, Bacterial↗
PubMed1988

An investigation into alternative methods for the serodiagnosis of dourine.

The complement fixation test (CFT), indirect fluorescent antibody test (IFAT), card agglutination test for trypanosomiasis (CATT) and enzyme-linked immunosorbent assay (ELISA) were compared in their application to the serological diagnosis of Trypanosoma equiperdum infection in 43 horses. The CFT remains a reliable test for dourine, especially in countries where other members of the subgenus Trypanozoon do not occur. The IFAT is a good 'back-up' test, but, requiring skilled operators it has the disadvantage of making it labour intensive, and interpretation of results subjective. This makes it more suited to small numbers of samples. The ELISA is suitable for large numbers of samples and could readily be used in routine diagnostic procedures. The CATT could be of value in field situations, although it does not appear to be as sensitive as the CFT. Its possible application under these conditions should be further investigated.

Agglutination Tests↗
PubMed1981

[Environmental influences on fertility (author's transl)].

The different environmental influences which can lead to fertility disturbances are set down in the form of a survey. Using literature and the results of our own investigations, particular reference is made to the possible influence of plant-protecting agents and tobacco consumption. The necessity of clarification regarding health condition is stressed.

Environment↗
PubMed1981

[Use of no-protein blood extract for infertility and sterility (author's transl)].

In vitro studies into the effects of various concentrations of Solcoseryl (Solco, Basle, Switzerland) on selected asthenozoospermia patients have shown rise in motility in 26 per cent of the probands and decline in motility in 17 per cent. Spermatozoan motility was most strongly increased by a five per cent concentration of Solcoseryl.--Fifty-one patients, from whom different spermiographic findings had been earlier obtained, received ten injections of one ampoule of Solcoseryl daily, with weekend pauses inserted. Included were 46 oligozoospermia, 31 asthenozoospermia, and eight hypospermia. Spermiographic checks were undertaken on termination of therapy, and others followed two weeks, four weeks, three months, and six months after termination of therapy. They all gave improved findings, which were most unambiguous with regard to the number of spermia.--Findings were at least temporarily normal after treatment in 58 per cent of the patients with asthenozoospermia. The wives of eleven in 44 patients (25 per cent) with Solcoseryl treatment were pregnant after three months, as established in follow-up examinations. Artificial insemination was applied to 45 patients, among them 37 with maritogenic approach and eight cases with donogenic approach, using Solcoseryl. Eleven pregnancies were obtained from maritogenic insemination. Pregnancy was achieved in 38 per cent of the probands who received cryopreserved donor semen. These findings would not justify the claim of direct conception-producing action for Solcoseryl, in the context of insemination treatment.

Actihaemyl↗
PubMed1981

[Serum concentration of pregnancy-associated alpha 2-glycoprotein (alpha 2-PAG, protein of pregnancy zone, PZ) in patients with dermatological diseases (author's transl)].

Radial immunodiffusion, according to Mancini, was applied to 15 hospitalised patients, both during and after hospitalisation. They were of both sexes and all afflicted with dermatological diseases (psoriasis, eczema, mycosis). Determination of serum concentration of pregnancy-associated alpha 2-glycoprotein (alpha 2-PAG, PZ) was the purpose of the tests. While in the acute phase of the disease the PZ serum levels of the three groups of patients were higher than those recorded from a control group, conventional therapy failed to have any impact upon PZ serum concentration. Continuous decline in PZ serum concentration was recordable from psoriasis patients in response to photochemotherapy (PUVA).

Female↗