PubMed Health⌕ Search

Biomedical subjects

A Merits

Publications and source records attributed to A Merits.

22 records · Page 2Linked to original sources
PubMed1994

Computer-assisted predictions of the secondary structure in the plant virus single-stranded DNA genome.

Coconut foliar decay virus (CFDV) contains the single-stranded circular DNA molecules of 1291 nucleotides which were found to replicate autonomously in the cells of the diseased palms. The special features of the CFDV DNA sequence, including putative secondary structure and the distribution of the inverted repeat motifs, are investigated with computer-assisted prediction methods. It is evident that the structural principle of the branched series of long and short double helixes interspersed by short non-helical regions is existed for CFDV virion DNA. The total degree of base pairing is near 62%. We have also predicted the presence of several sequence elements formed by inverted repeat motifs which are potentially capable of binding the eukaryotic transcriptional regulatory factors.

Base Sequence↗
PubMed1994

Genome characterization and taxonomy of Plantago asiatica mosaic potexvirus.

The complete nucleotide sequence of Plantago asiatica mosaic virus (P1AMV) genomic RNA has been determined. The 6128 nucleotide sequence contains five open reading frames (ORFs) coding for proteins of M(r) 156K (ORF1), 25K (ORF2), 12K (ORF3), 13K (ORF4) and 22K (ORF5). The sequences of these P1AMV proteins exhibit strong homology to the proteins of the other potexviruses. Phylogenetic trees based on the multiple sequence alignments of three conserved domains in ORF1 product and capsid protein reveal a close relationship of P1AMV to papaya mosaic virus and clover yellow mosaic virus. The P1AMV genomic RNA and a major subgenomic RNA (sgRNA) of 0.9 kb have been detected in infected leaves by Northern blot hybridization. The latter sgRNA is the messenger for virus capsid protein and its 5' terminus has been located 23 nucleotides upstream of the initiator codon of the coat protein gene. The P1AMV virion RNA and RNA transcript resembling the 0.9 kb sgRNA have been translated in vitro giving rise to a single major 170K product and a major 22K product, respectively.

Amino Acid Sequence↗
PubMed1994

Computer search of transcription control sequences in small plant virus DNA reveals a sequence highly homologous to the enhancer element of histone promoters.

The positions of nucleotide sequences which can act as binding sites for plant transcriptional trans-acting protein factors have been mapped in DNA of plant circovirus--coconut foliar decay virus (CFDV). It was found that CFDV promoter region contains sequence motif homologous to the regulatory type I element of plant histone genes. We have also found the presence of the element I motifs in DNAs of other small plant viruses. Taking into account the mechanism of regulation of histone gene expression it appears that these sequences may play a role in a cell cycle-dependent regulation of plant virus DNA transcription and replication.

Base Sequence↗