Biomedical subjects

A Michalska

Publications and source records attributed to A Michalska.

10 recordsLinked to original sources

PubMed2001

Interference of sulfate(VI) with poly(pyrrole) electrochemistry.

The effect of the SO4(2-) anion on both potentiometry and voltammetry with poly(pyrrole) films doped with chloride or hexacyanoferrate(II/III) ions has been studied and compared with the responses obtained in KCl solutions. Strong interference was observed when the SO4(2-) to Cl- molar ratio was 1:1000, leading to a decrease in polymer film electroactivity at higher sulfate concentrations. The effect is attributed to incorporation of the divalent anion into the polymer film structure, a hypothesis supported by analysis of the composition of the elemental film.

Journal Article↗

PubMed2001

[Cloning - controversies].

Cloning of the human being is not only highly controversial; in the opinion of the authors it is impossible - we are not able to reproduce human behaviour and character traits. Reproduction through cloning is limited to personal genome resources. The more important is protection of genomic characteristics as private property and taking advantage of cloning for production of the human organs directly or through xenotransplants. In this paper we present the legislation related to cloning in Poland, in the European Union and other countries. We also indicate who and why is interested in cloning.

Bioethical Issues↗

PubMed1998

Novel method for demonstrating nuclear contribution in mouse nuclear transfer.

Confirmation of nuclear contribution is essential to all nuclear transfer experiments. Contribution is easily demonstrated in nuclear transfer progeny but more difficult to confirm in nuclear transfer embryos. The use of donor nuclei isolated from lacZ transgenic mice offers a clear and simple method to demonstrate contribution in nuclear transfer embryos and offspring. The unique line of transgenic mice (Zin40) used in this study displays nuclear localised lacZ expression in all cells, including embryonic blastomeres, and demonstrates distinctive blue nuclei when treated with X-gal substrate. This characteristic staining pattern provided an ideal marker for demonstrating nuclear contribution. Nuclear transfer embryos were generated following serial nuclear transfer of metaphase-arrested nuclei from transgenic and non-transgenic 4-cell embryos. Totipotency of nuclear transfer blastocysts was confirmed by the generation of live born offspring. Transgenic blastocysts and all tissue samples from fetuses and pups generated by nuclear transfer displayed distinctive blue nuclei when stained with X-gal. This staining pattern was characteristic of the transgenic mice from which the donor nuclei were isolated and clearly confirmed nuclear origin. The use of this marker will also allow the opportunity to investigate the developmental potential of nuclear transfer embryos by examining the contribution of nuclear transfer embryonic cells in chimaeric embryos.

Animals↗

PubMed1998

[Results of five year care of newborns born from diabetic mothers].

Diabetic fetopathy is a complex the pathophysiology of which is not all that clear and that is why all neonates whose mothers develop diabetes demand--regardless of their body weight--from the first hours of their live an intensive neonatal care. In the period from 1993 to 1997 in the Chair I of the Hospital of Obstetrics and Gynaecology in Bytom 191 babies were born in whom--in the first day of their live clinical and biochemical screening was carried out in order to determine degree of diabetic fetopathy progression. Summary results of five years care of newborn, born by diabetic mothers. The following parameter were measured: morphometric parameters, the course of adaptation processes, congenital defect occurrence, morbidity and motility in this group of newborn.

Apgar Score↗

PubMed1995

Endotoxin-induced inflammation does not cause hepatic zinc accumulation in mice lacking metallothionein gene expression.

The action of endotoxin lipopolysaccharide (LPS) on hepatic Zn uptake was examined in mice lacking expression of metallothionein (MT)-1 and MT-II genes. Hepatic Zn concentrations, which in normal control mice increased by a mean 29% (MT elevated 20-fold) 16 h post-LPS exposure, did not increase in MT-null mice. Plasma Zn fell by 68% in controls and 32% in MT-null mice. The time course of LPS action in normal mice was characterized by a rapid reduction (-74% at 4 h, -81% at 8 h) and partial recovery (-39% at 24 h) in plasma Zn, with a progressive increase over 24 h in hepatic concentrations of MT (by 36-fold) and Zn (by 40%). In contrast, the MT-null mice had a linear decrease in plasma Zn (-15% at 8 h, -41% at 24 h) and early loss of Zn from the liver. The Zn changes seen in MT-null mice were largely attributable to LPS-associated anorexia. Food deprivation (20 h) alone caused respective 14% and 30% decreases in hepatic and plasma Zn concentrations and a 27% reduction in total liver Zn reserves, whereas fasted normal mice conserved Zn with a 4-fold increase in hepatic MT. This study confirms that MT synthesis is essential for endotoxin-induced liver Zn accumulation.

Animals↗

PubMed1987