PubMed Health⌕ Search

Biomedical subjects

A Misaki

Publications and source records attributed to A Misaki.

86 records · Page 5Linked to original sources

[Glucan of yeast].

Explore the source record for details and available documents.

Chemical Phenomena↗

Formation of isoamylase by Pseudomonas.

We have isolated a Pseudomonas sp. (strain SB15) which produces an isoamylase (EC 3.2.1.9). Highest yields of this enzyme were obtained when the bacterium was grown in shaken culture in a medium containing maltose, dextrin, starch, or isomaltose. Specific carbon and nitrogen sources were required for growth. The most satisfactory medium consisted of 2% maltose, 0.4% sodium glutamate, 0.3% diammonium hydrogen phosphate, and other inorganic salts. The optimal pH for enzyme production was 5 to 6. The enzyme is stable between pH 3 and 6 but is extremely labile above pH 7. It splits amylopectin completely by combined action with beta-amylase but does not attack pullulan.

Culture Media↗