Plasma levels of diftalone in eight animal species evaluated by a TLC-spectrophotometric assay method.
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Biomedical subjects
Publications and source records attributed to A Morrone.
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A method is described for the determination of diftalone in human plasma, based on gas chromatography of a purified diethyl ether extract. The procedure, which has been designed for use with an automatic sampler, allows the assay of diftalone at plasma levels as low as 0.5 mug/ml.
Twenty apparently healthy homosexuals from Italy and five patients with lymphadenopathic syndrome (LAS) were studied for specific cell mediated immunity (CMI) to Cytomegalovirus (CMV), by means of the leukocyte migration inhibition test (LMIT). Total circulating T cells and T cell subpopulations were also investigated by the use of specific monoclonal antibodies of the OK series. An inverted OKT4/T8 ratio was also found in about 30% of subjects. CMV was recovered from the urine of three homosexuals and high specific antibody titers were found in all the people studied. In addition we found a lack of specific CMI to CMV in 18 homosexuals and in 3 patients with LAS. We think that CMV can induce a state of immune tolerance in people at risk for AIDS which may contribute to promote the disease.
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