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A Moussa

Publications and source records attributed to A Moussa.

29 records · Page 2Linked to original sources
PubMed1993

Identification of Moraxella-like bacteria isolated from caprine and ovine nasal flora.

Twenty four Moraxella related bacterias were isolated from healthy caprine and ovine nasal swabs and were investigated by classic biochemical tests and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins in comparison with 9 reference strains. Proteolytic and haemolytic strains were investigated by electron microscopy. The biochemical results clustered field isolates in four groups corresponding to Branhamella and Moraxella species. Proteolytic, haemolytic and fimbriated field isolates showed the same morphological structure and biochemical features as Moraxella bovis. SDS-PAGE results indicated that DICE coefficient between a field isolate and the corresponding reference strain can be as 62.5%; 41.7% and 36% respectively for the groups 1, 3 and 4. The group 2 showed a similarity percentage over 75% with the reference strain Moraxella nonliquefaciens. This results indicated that a non proteolytic but haemolytic bacteria, closely related to Moraxella nonliquefaciens was commonly isolated from small ruminants nasal flora. These animals can also be hosts of a subspecies of Moraxella bovis.

Animals↗
PubMed1992

Haemorrhagic disease of lagomorphs: evidence for a calicivirus.

Studies on the aetiological agents of rabbit haemorrhagic disease (RHD) and European brown hare syndrome show that the viruses responsible for these infections can be placed in the family Caliciviridae. Established members of this group are vesicular exanthema virus (prototype), San Miguel sea lion virus and feline calcivirus. The human hepatitis E virus and the Norwalk agent may soon be included. The RHD virus genome consists of a positive stranded RNA molecule composed of 7437 nucleotides. A major subgenomic RNA of 2.2 kb, colinear with the 3' end of the genomic RNA, can also be recovered from infected liver tissue, and both RNAs are enclosed within viral capsids formed by a single major protein of approximately 60 kDa. Electron microscopic examination of organ suspensions from diseased animals shows two types of particle; 35-40 nm complete virions have the regularly arranged cup-shaped depressions typical of calcivirus morphology, and 23-25 nm smooth particles resulting from degradation of the outer surface structures of the complete virions.

Animals↗
PubMed1992

[Distribution and importance of sites of Glossina fuscipes fuscipes Newstead 1910 in the agglomeration of Bangui (Central Africal Republic)].

The authors review urban sites of Glossina fuscipes fuscipes in the suburbs of Bangui and propose a classification of these sites. Among them, two isolated breeding sites are characterized by a high density of tsetse and a close man/fly contact. To prevent a possible reactivation of the historical sleeping sickness focus of Bangui-Bimbo, a tsetse control programme in these two sites is necessary.

Animals↗
PubMed1991

Detection of the bovine leukaemia virus by the polymerase chain reaction.

The polymerase-chain reaction was applied for detection of provirus DNA of the bovine leukaemia virus (BLV). A short fragment of 292 bp including region R and U5 LTR 5' of BLV was amplified, and the optimum parameters of amplification of this fragment were established. Electrophoresis revealed the presence of the 292 bp fragment from the leucocytes of four out of six cows showing a positive serological response to BLV antigens. Application of the polymerase-chain reaction in diagnosis of bovine leukaemia is suggested.

Animals↗
PubMed1990

Round table on infectious bovine rhinotracheitis/infectious pustular vulvovaginitis virus infection diagnosis and control.

The current situation of infectious bovine rhinotracheitis/infectious pustular vulvovaginitis infection in various European countries is reviewed. Whilst some have a high serological prevalence and use live virus vaccines to control the disease, others have a low prevalence and two countries (Denmark and Switzerland) have national eradication schemes which are almost complete. Serology remains important for diagnosis although other tests such as delayed cutaneous hypersensitivity may have a role to play. New tests such as polymerase chain reaction may find increasing application where high sensitivity is required, such as the detection of virus in semen.

Animals↗
PubMed1985

[Laboratory diagnosis of respiratory diseases of cattle].

The authors describe the procedure of laboratory diagnosis for bovine respiratory diseases: direct diagnosis by isolation and for identification of bacteria or viruses and indirect diagnosis by serological methods. They specify the restraints and limits of this diagnosis and the significance results which are obtained and connected with knowledge of anamnestic information.

Animals↗
PubMed1976

[Serological study of several strains of foot-and-mouth disease virus type "O" isolated in Europe between 1971 and 1975: application of the biomathematical system of classification].

Nine strains of foot-and-mouth disease virus type "O" received in our laboratories since 1971 have been studied serologically by Osler's quantitative method of complement fixation (50% hemolysis). The results, submitted to the biomathematical system of bidimensional classification, allow to conclude that at present in Europe there are two groups of foot-and-mouth disease strains of type "O"; one has reference to our vaccinal strain "O Lausanne 1965" and the other to "O Romania 1972" strain, which has certain points of similarity with subtype "O2". In addition, strain "O Hungary 1975" has been studied on bovines; the immunological tests confirm the serological test.

Aphthovirus↗
PubMed1976

[Classification of strains of foot-and-mouth disease virus according to the relationship-dominance model for a better understanding of the concept of serologic and immunologic subtype].

The homologous and heterologous serological titers of a specific serum can be integrated in a bidimensional system which characterizes the criteria of relationship and dominace. This bidimensional classification specifies more precisely the serological properties of each strain compared with the others (taxonomic purpose) and allows eventual guidance in the choice of vaccinal strains (epizootiologic and prophylactic purposes).

Aphthovirus↗
PubMedDate not supplied

Immortalized goat milk epithelial cell lines replicate CAEV at high level.

Primary milk epithelial cells were isolated from CAEV-uninfected goats and three cell lines designated TIGMEC-1, TIGMEC-2 and TIGMEC-3 were established. The three cell lines retained their morphological characteristics of epithelial cells and expressed specific epithelial cytokeratin marker as well as the immortalizing SV40 large T antigen. The kinetics of growth of TIGMEC1, TIGMEC2 and TIGMEC3 cell lines showed a doubling time of 24-48 hours while the parental cell lines became senescent after the passage 6 in cell culture. Like the parental primary cells, the three cell lines were found to be highly sensitive to CAEV-pBSCA, an infectious molecular clone of CAEV-CO strain, and to a French isolate CAEV-3112. TIGMEC cell lines infected with CAEV-pBSCA became chronically infected producing high virus titers in absence of cytopathic effects. These cell lines may be useful for study of the possible physiological alterations in mammary epithelial cells infected with small ruminant lentiviruses and their consequences on milk quality. On an other hand, these cell lines can be used to study their role in virus transmission and pathogenesis.

Animals↗
PubMedDate not supplied

[A scrotal tumor].

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Adult↗