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Biomedical subjects

A Mrozikiewicz

Publications and source records attributed to A Mrozikiewicz.

At least 19 recordsLinked to original sources
PubMed2001

[Polymorphism of gene angiotensin converting enzyme in pregnancy induced hypertension].

In the recent years genetic background of pregnancy induced hypertension (PIH) are intensively investigated. Genetically determined differences in activity of renin-angiotensin system (RAS) are of importance to hypertension susceptibility. The insertion/deletion (I/D) polymorphism of angiotensin I converting enzyme (ACE) was suggested to play an important role in the aetiology of idiopathic hypertension. We have tested if this polymorphism could be associated with PIH. ACE polymorphism was investigated in 87 pregnant women with PIH and in 110 healthy pregnant women (control group). Investigation was performed by polymerase chain reaction (PCR). We have amplified genomic DNA excteracted by phenol-chloroform method from blood leucocytes. We have detected overrepresentation of the I allele in the PIH group (47.2% and 41.4% in PIH and controls, respectively). ACE genotype frequency in control group was in agreement with expected values, according to Hardy-Weinberg law, but in the PIH group the obtained values were different from expected. This observation confirmed the possible role of I allele in aetiology of PIH, and we believe that continuation of this investigation is necessary.

Adolescent↗
PubMed1999

Microvascular protective activity of flavonoid glucuronides fraction from Tulipa gesneriana.

A mixture of flavonoid glucuronides, consisting of 7-O-glucuronides of kaempferol and quercetin 3-O-rutinosides, 3-O-gentiobiosides and 3-O-glucosides, was isolated from the perianths of Tulipa gesneriana L. var. 'Paradae'. It showed protective activity against the increased (both chloroform and histamine) skin vascular permeability in rabbits. The protective effect, measured as the reduction in leakage of Evans blue, was 59.8% after peritoneal treatment at a dose of 25 mg/kg, while that of troxerutin was 45.5%.

Animals↗
PubMed1999

Intense acute phase response in ischemic patients.

The aim of this study was to estimate the qualitative and quantitative changes of acute phase proteins in patients suffering from coronary heart disease. The study was carried out on 74 patients and 12 healthy volunteers. The patients were divided into three groups as follows: patients with myocardial infarction (n=37), Group I--without heart failure, Group II--with heart failure (II-III NYHA), Group III--patients with unstable angina pectoris (n=35); controls-healthy volunteers (n=12). The immunological measurements were performed at the beginning of hospitalisation (point 0), after 4, 8, 12 and 72 h, and after 6, 9 and 12 days of hospitalisation. The concentrations of C-reactive protein (CRP), alpha1-acid glycoprotein (AGP) and alpha1-antichymotrypsin (ACT) were measured using rocket immunoelectrophoresis according to Laurell. Glycosylation profiles of AGP and ACT were determined using crossed affinity immunoelectrophoresis with Con A as ligand according to Bøg-Hansen. Between Groups I and II statistically significant differences were observed for all investigated parameters. Highest concentration values were observed for Groups II and III; for Group II they appeared earlier than for Group III. The maximal values for reactivity coefficients (AGP-RC and ACT-RC) were observed earlier than the respective maximal values of concentrations. Continuous activation occurring in unstable angina leads to a more rapid increase in the concentrations of acute phase proteins and more marked alterations in their glycosylation profiles. In a way these patients seem to be 'primed' with constant stimulation, so that they respond dramatically to the stimulus of ischemia.

Acute-Phase Proteins↗
PubMed1999

Mutations in the human paraoxonase 1 gene: frequencies, allelic linkages, and association with coronary artery disease.

Oxidative damage is a major cause of atherosclerosis. Since human paraoxonase has been postulated as a factor which plays a role in protection from low density lipoprotein oxidation, recent studies have dealt with the impact of hereditary PON1 gene polymorphisms as risk factors for coronary artery disease (CAD). The results from these studies are conflicting. In a case-control study, 1000 Caucasian patients with angiographically confirmed CAD were recruited and matched by age and gender to 1000 control individuals. PON1 mutations in codons 55 and 192 were evaluated by polymerase chain reaction-restriction fragment length polymorphism and allocated to defined haplotypes *1 (55L/192Q), *2 (55L/192R), and *3 (55M/192Q). Frequency of PON1 genotypes without any mutation (PON1*1/*1, wild-type) in CAD cases was 16.9% versus 17.1% in control individuals. PON1*2/*2 showed a frequency of 6.6% versus 7.3% (P = 0.68 compared to wild-type), and PON1*3/3 occurred in 11.8% in CAD cases versus 10.3% among control individuals (P = 0.40). There was also no difference in the distribution of carriers heterozygous for *2 or *3 among cases and control individuals. A haplotype containing both mutations 55M and 192R was not observed. None of the investigated genotypes demonstrated association with early manifestation, severity of disease, acute coronary syndromes, or myocardial infarction. Logistic regression analysis with adjustment for age, gender, diabetes, hypertension, hypercholesterolemia and smoking revealed no evidence of increased coronary risk associated with PON1 genotypes. These results suggest that PON1 polymorphisms are not major genetic determinants of CAD.

Alleles↗
PubMed1994

Blood levels of alloxan in children with insulin-dependent diabetes mellitus.

Alloxan is a well-known and universally used agent for evoking experimental diabetes through its toxic effect on the B cells of the Langerhans islets. In our study, blood levels of alloxan in children with insulin-dependent diabetes mellitus were investigated. The observations were made in 68 children aged 6-15 years and in a control group of 44 healthy children in the same age range. Alloxan levels were estimated spectrophotometrically. The mean level of alloxan in blood from children with insulin-dependent diabetes mellitus was 8.76 +/- 9.64 micrograms/ml and in blood from healthy children was 1.53 +/- 1.10 micrograms/ml. The difference was statistically significant (P < 0.05). The metabolism of alloxan leads to the production of free superoxide radicals which, as is well known, injure cells and cause conditions conducive to the occurrence of diseases from autoimmunity. The results obtained suggest therefore that higher levels of alloxan in diabetic children are of significance in the onset of insulin-dependent diabetes mellitus.

Adolescent↗
PubMed1989

Assessment of liver ability to biotransformation of antipyrine in uraemic patients on regular peritoneal dialysis treatment.

It was the aim of this work to establish whether biotransformation of drugs by the liver expressed by antipyrine kinetics is disturbed in peritoneally dialysed patients with end-stage renal failure. The investigations were carried out in 10 uraemic patients using the antipyrine test and comparing the parameters of antipyrine kinetics with those obtained in 13 healthy persons. Our results indicate that in uraemic patients on regular peritoneal dialysis treatment antipyrine kinetics are generally in the normal range, suggesting the microsomal content of cytochrome P-450 being not evidently reduced.

Antipyrine↗
PubMed1983

Synthesis and biological action of some pyrido-[1,3]-diazepine and pyrido-[2, 3-d]-pyrimidine derivatives.

The following compounds were synthesized and tested for their action on the circulatory system and smooth muscle preparations: 3H,4,9-dihydropyrido-[2,3-e] [1,3]-diazepino-4,9-dione 1a, 3,4-dihydropyrido-[2,3-d]-pyrimidinone-4 2a and their derivatives: N-methylmorpholine 1b and 2b, N-methylopiperidine 1c and 2c, N-beta-hydroxy-gamma-morpholinopropyl 1d and 2d, and N-beta-diethylaminoethyl 1e and 2e. Compounds 1b-e and 2c showed weak hypotensive action in rats when given in a dose of 50 mg/kg, compounds 2d and 2e: 30 mg/kg and up.

Animals↗
PubMed1975

Influence of the vaccine Polyvaccinum mite on the nasal mucosa.

The necessity of prolonged administration of the vaccine Polyvaccinum prompted this study on the effect of the preserving agent on the nasal mucosa. Buffered physiologic saline solution containing 0.1--0.4% phenol, and the vaccine containing 0.1--0.4% phenol or 0.01% merthiolate were applied for 12 weeks on the nasal mucosa of rabbits. The nasal mucosa, parenchymal organs and adrenals were studied macroscopically and histologically, and immunologic studies were made. On the basis of the results, preservation of Polyvaccinum mite with 0.4% phenol is suggested.

Administration, Intranasal↗
PubMed1975

Influence of some antidepressant drugs on the circulatory system. I. Imipramine.

Imipramine exerts various, dose-related, effects on arterial blood pressure. A distinct hypotensive effect occurs at dosses of at least 0-5 mg/kg body weight. At lower dosage, the action of the drug is biphasic. In experiments in situ with animals, imipramine diminished amplitude of the heart's contractions. Controlled respiration during experiments with imipramine reduced its toxicity. In decapitated animals the action of imipramine was the same as in animals with intact central nervous system. At all dosage levels its effect on blood pressure was biphasic. Blockade of the sympathetic, parasympathetic system and vegetative ganglia had no significant effect on the circulatory response to imipramine. Low doses of imipramine potentiated the hypertensive effect of noradrenaline, and high doses weakened it.

Animals↗
PubMed1975

Influence of imipramine on the circulatory system in the course of endogenous depressive syndromes. II. Influence of imipramine on vascular reflexes.

In patients with endogenous depression imipramine normalizes vascular responses in vegetative tests (orthostatic test, Schellong's test, cold pressor test). The highest percentages of normal vegetative tests during treatment with imipramine were observed in patients in remission of symptoms of depression. The orthostatic tests seem to have the highest diagnostic value. In tests based on measurement of surface temperature imipramine improved function of the vascular system.

Adult↗
PubMed1975

Influence of imipramine on the circulatory system in the course of endogenous depressive syndromes. I. Evaluation of the basic parameters of the circulatory system.

In patients with endogenous depression, imipramine exerts a regulative effect in both directions on blood pressure and pulse rate, dependent on the starting levels. In a majority of patients treated with imipramine, after 14 days blood pressure dropped and pulse rate was slowed. Remission of endogenous depression was statistically significantly more frequent in patients treated with imipramine in whom pressure and pulse rate underwent normalization. Early appearance of these effects of imipramine in patients with endogenous depression seems to be a good prognostic index.

Adult↗