Biomedical subjects
Publications and source records attributed to A N Bondarenko.
Described in the paper is a micromethod for the determination of the activity of proteinases in blood serum by an ELISA-based reader. BAPNA was used as substrate for proteolytic enzymes. The method is simple, easily reproducible and cost effective--it fits for large-scale examinations.
Instructions on assembling a high-quality and inexpensive microscopy video system that can be made independently are described in the paper. Recommendations on tuning and using the video set are defined alongside with the methods of modernizing the system.
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The use of the serological method (the indirect fluorescent antibody test) for the examination of 255 persons coming in contact with patients having hemorrhagic fever with the renal syndrome (HFRS) in the foci of this infection where outbreaks of group infection had been registered made it possible to detect mild, unclear and asymptomatic forms of HFRS in 17.7-20.9% of cases. The simultaneous titration of 317 serum samples with two antigens revealed that HFRS virus of serotype 1, similar to strain Hantaan from Korea, may cause, besides the classical forms of the disease, the development of mild and asymptomatic forms of this infection.
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The employment of two serological tests (indirect fluorescence antibody technique and neutralization test) demonstrated the leading role of Hantaan virus serotype 1 strains isolated from field mice in the pattern of the incidence of hemorrhagic fever with renal syndrome (HFRS). Proofs of the etiological importance of strains of serotypes 3 and 5 occurring in brown rats and Cl. rufocanus were obtained. No data on any association of human HFRS cases with strains of serotype 4 isolated from reed voles could be demonstrated.